Yanni Kussuryani
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BUFFER REPLACEMENT AND SIMULTANEOUS SACCHARIFICATION AND FERMENTATION ON BIOBUTANOL PRODUCTION?FROM LIGNOCELLULOSIC BIOMASS Devitra Saka Rani; Yanni Kussuryani
Scientific Contributions Oil and Gas Vol 39 No 3 (2016)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.39.3.99

Abstract

Lignocellulosic biomass is excellent feedstock for biofuel such as biobutanol. Bagasse, rice straw, and empty fruit bunch (EFB) oil palm are untapped potential for biobutanol production as gasoline blending/ substitution. However, biobutanol production by fermentation from lignocellulosic biomass is a process that consumes time and energy which leads to high production costs. This research is intended to optimize biobutanol production that reduces production costs, an important factor on an industrial scale. Optimization is conducted by replacing the buffer solution in enzymatic hydrolysis with distilled water and by using Simultaneous Saccharification and Fermentation (SSF). The results showed that the buffer replacement with distilled water can reduce cost by approximately 41,726 IDR/liter hydrolysate. Biobutanol contents from all biomass of bagasse, rice straw, and EFB oil palm are higher using SSF compared to Separate Hydrolysis and Fermentation (SHF). The SSF system can cut production time by 3 days and save electricity of 32.4 kWh.?
Seleksi Mikroba dan Nutrisi yang Berpotensi Menghasilkan Biosurfaktan untuk MEOR Cut Nanda Sari; Yanni Kussuryani
Lembaran publikasi minyak dan gas bumi Vol 47 No 2 (2013)
Publisher : BBPMGB LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/LPMGB.47.2.222

Abstract

Biosurfaktan merupakan surfaktan yang dihasilkan oleh mikroba dari golongan bakteri hidrokarbonoklastik yang memiliki kemampuan menurunkan tegangan antar muka. Faktor keberhasilan dalam produksi biosurfaktan ditentukan dari jenis mikroba dan nutrisi yang digunakan. Kegiatan penelitian ini terdiri atas empat tahapan yaitu aktivasi dan kultivasi mikroba, seleksi mikroba penghasil biosurfaktan, kurva pertumbuhan mikroba, seleksi nutrisi. Aktivasi dan kultivasi mikroba dilakukan dalam tiga tahapan dengan masa inkubasi masing-masing tahapan yaitu 37oC selama 24 jam. Hasil seleksi mikroba penghasil biosurfaktan diperoleh tiga jenis mikroba dari tujuh mikroba yang diuji, berdasarkan indikasi luasnya zona lisis yang terbentuk pada media agar darah yaitu, BLCC B-3, BLCC B-4 dan BLCC B-5. Hasil uji lanjut terhadap ketiga mikroba tersebut pada media minyak dengan mengukur tegangan antar muka (IFT), menghasilkan dua mikroba dengan nilai IFT yang terendah yaitu BLCC B-3 dan BLCC B-5. Hasil screening nutrisi berdasarkan pengukuran Tegangan Antar Muka (IFT), Viskositas, Total Plate Count (TPC), dan pH, menunjukkan media BC-4 dan media PA-4 mendukung aktivitas mikroba dalam memproduksi biosurfaktan. Biosurfactant is a surfactant derived from hydrocarbonoclastic bacteria which are capable to reduce surface tension. The successful biosurfactant productions are determined by nutrition and microbial species. This research consists of 4 main steps: activation and cultivation of microbes, microbial growth curves, screening of surfactant producing bacteria, and screening of nutrition. Microbial activation and cultivation conducted in 3 sequential cultivation in 24 hours incubation time at 37oC. Screening of surfactant producing bacteria from 7 microbial isolates obtained 3 isolates which show positive result based on diameter of hemolytic area on blood agar. They are BLCC B-3, BLCC B-4 and BLCC B-5. The interfacial tension (IFT) examination result from these 3 isolates showed that BLCC B-3 and BLCC B-5 had the lowest IFT value. The result of nutrition screening based on IFT, viscosity, Total Plate Count, and pH show that BC-4 and PA-4 media are the best composition of media that support the microbes in producing surfactants.
Reduksi Gas CO2 oleh Mikroalga Scenedesmus sp. pada Fotobioreaktor Tertutup dengan Variasi Konsentrasi Gas CO2 Rino Nirwawan; Yanni Kussuryani; Dhiti Adiya Hanupurti
Lembaran publikasi minyak dan gas bumi Vol 48 No 1 (2014)
Publisher : BBPMGB LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/LPMGB.48.1.230

Abstract

Salah satu metode potensial yang dapat digunakan untuk reduksi CO2 adalah memanfaatkan aktivitas mikroalga melalui proses fotosintesis. Mikroalga adalah bioagen yang mampu menangkap CO2 dan mengubahnya menjadi karbohidrat untuk menambah pertumbuhan populasinya. Banyaknya CO2 yang digunakan dapat mencapai hampir dua kali lipat dari berat kering biomassa yang dihasilkan. Tujuan kegiatan ini adalah mengkaji kemampuan mikroalga Scenedesmus sp dalam mereduksi gas CO2 pada suatu fotobioreaktor skala pilot dengan memvariasikan konsentrasi gas CO2 yang diinjeksikan ke dalam sistem. Penelitian dilakukan di Lapangan Gas Subang selama tujuh hari. Komposisi gas CO2 yang digunakan adalah ±98%. Sistem operasi adalah sistem batch dan media pertumbuhan yang digunakan adalah media “Sederhana 2”. Pada penelitian ini digunakan empat rangkaian fotobioreaktor dengan volume operasi masing-masing adalah 60 Liter. Masing-masing fotobioreaktor divariasikan perbandingan jumlah gas CO2 dan udara yang diinjeksikan, yaitu 0:100% (fotobioreaktor 1) yang berfungsi sebagai kontrol, 10:90% (fotobioreaktor 2), 30:70% (fotobioreaktor 3) dan 50:50% (fotobioreaktor 4). Kepadatan sel, optical density (OD), pH, dan berat kering digunakan sebagai parameter pengujian. Hasil penelitian menunjukkan bahwa reduksi gas CO2 tertinggi terdapat pada fotobioreaktor 2 yang terjadi pada hari ke-3 operasi, yaitu sebesar 8,09x10-5 gram dengan nilai kepadatan sel 23,87 x 106 sel/mL. Dari hasil tersebut dapat disimpulkan bahwa penambahan 10% gas CO2 ke dalam fotobioreaktor dapat meningkatkan pertumbuhan mikroalga Scenedesmus sp. One potential method that can be used for the reduction of CO2 is utilizing the microalgae activity through the process of photosynthesis. Microalgae is a bioagen that is able to capture the CO2 and convert it into carbohydrates for the growth of the population. The number of CO2 used can achieve almost double of the dry weight biomass produced. The purpose of this study is to assess the ability of Scenedesmus sp. microalgae in the reduction of CO2 gas at a pilot scale photobioreactor by varying the concentration of CO2 to be injected into the system. The research was done in Subang gas eld for seven days. The composition of the CO2 gas was ±98%. The operating system was a batch system and used ”Sederhana 2”as a growth media. In this study we were using four sets of photobioreactor with their respective operating volume of 60 liters. Each photobioreactor has a different ratio of CO2 gas and air to be injected, that is 0: 100% (1st photobioreactor) that serves as the control, 10:90% (2nd photobioreactor), 30:70% (3rd photobioreactor) and 50:50% (4th photobioreactor). Cell density, optical density (OD), pH, and dry weight were used as test parameters. The result showed that the reduction of the highest CO2 gas contained on 2nd photobioreactor which occurs on the 3rd day of the operation, i.e. by 8.09x10-5 gram with cell density of 23.87 x 106 cell/ mL . From these results it can be concluded that the addition of CO2 into the photobioreactor can increase the growth of microalgae Scenedesmus sp.