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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Folia Medica Indonesiana
Prasasti, Adelia Gita
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Social Sciences

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Lymphocyte Count and SARS-CoV-2 Antibody Level in Healthy Donors' Blood at an Indonesian Blood Transfusion Center Prasasti, Adelia Gita; Woelansari, Evy Diah; , Suhariyadi; Anggraini, Anita Dwi
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. It is essential to conduct research on SARS-CoV-2 for the purpose of acquiring further understanding, especially concerning the production of antibodies examined using antibody titer blood tests. 2. Although the relationship between the examined variables is not significant, this study offers valuable information on blood test results after the COVID-19 vaccination, which can serve as scientific evidence for further research. Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that infects the respiratory system by attacking the mucous and epithelial cells. This infection commonly leads to an increase in lymphocyte count as an immune response to invading pathogens. Moreover, antibodies bind and inactivate foreign substances to destroy pathogens and inhibit their replication. These mechanisms prompt the objective of this study, which was to define the relationship between lymphocyte count and SARS-CoV-2 antibody level. This analytical observational study used a cross-sectional approach with quantitative analysis methods and purposive sampling. Healthy donors who had received coronavirus disease (COVID-19) vaccines provided the samples for this study. A total of 30 blood samples were collected from the Blood Transfusion Center of the Indonesian Red Cross Surabaya Area. This study was conducted in May 2022 at two distinct locations. The examination of lymphocytes was carried out using the flow cytometry method in the Hematology Laboratory, Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. In addition, the antibody titer test using the enzyme-linked immunosorbent assay (ELISA) method was performed in the Immunoserology Laboratory of the Surabaya Health Laboratory Center, Surabaya, Indonesia. The analysis revealed an average lymphocyte concentration of 2.2633 x 103/µl and an average antibody level of 0.2197 according to the optical density (OD) ratio. The data analysis was performed using Spearman's rank correlation statistical test (p<0.005), and the results indicated a lack of significance with p=0.262. In conclusion, there is no relationship between total lymphocyte count and SARS-CoV-2 antibody level.
Characterization of Aedes sp. larvae: Detection of dengue, Japanese encephalitis and West Nile virus with knockdown resistance mutations in Mulyorejo, Surabaya, Indonesia Prasasti, Adelia Gita; Sucipto, Teguh Hari; Kurniawan, Muhammad Ridho Hafid; Fauziyah, Shifa; Riris, Anak Agung Istri Dalem Cinthya; Djurumana, Yohana; Hapsari, Nafisah Nurul; Farihah, Neni Isna; Wijayanti, Alvia Rachma; Kusumawati, Nita; Hariyono, Hariyono; Wardhani, Puspa
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 1 (2026): Integration of Molecular Approaches in Addressing Drug Resistance and Changing Gl
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i1.6654

Abstract

Indonesia recorded the highest dengue mortality rate in Asia in 2022, with 45,387 cases and 432 deaths.  This study aimed to investigate the coexistence of arboviruses and insecticide resistance mechanisms in Aedes vectors mosquitos’ populations in Mulyorejo District, Surabaya, during the early monsoon season of 2021. A cross-sectional survey was conducted in January 2021, and Aedes larvae were collected and reared to adulthood under laboratory conditions. A total of 309 Aedes larvae (comprising 65% Aedes aegypti and 35% Aedes albopictus) were collected from 36 breeding sites, identified, and georeferenced using QGIS software. RNA extraction and RT-PCR assays were performed to detect Dengue virus (DENV), Japanese encephalitis virus (JEV), West Nile virus (WNV), and knockdown resistance (KDR) mutations. Results showed seven samples positive for DENV-2 and seven for JEV, with two samples (codes 9 and 12) exhibiting co-infection with both viruses, while no WNV was detected. The detection of JEV in Aedes larvae is a notable finding, suggesting a potential secondary vector role for these species in the study area, as Culex mosquitoes are the primary vectors of JEV. Mutations at codons 1016 (V1016G) and 1534 (F1534C), associated with pyrethroid and DDT resistance, were detected in eight and seven samples, respectively. These findings confirm the circulation of DENV-2 and JEV in local Aedes populations, highlighting the presence of KDR mutations that confer insecticide resistance. The results underscore the urgent need for integrated vector management in Surabaya, combining arbovirus surveillance, insecticide resistance monitoring, and community-based source reduction to mitigate arboviral disease transmission.
Co-Authors , Suhariyadi Anggraini, Anita Dwi Djurumana, Yohana Evy Diah Woelansari Farihah, Neni Isna Hapsari, Nafisah Nurul Hariyono Hariyono Kurniawan, Muhammad Ridho Hafid Nita Kusumawati Riris, Anak Agung Istri Dalem Cinthya Shifa Fauziyah Teguh Hari Sucipto, Teguh Hari Wardhani, Puspa Wijayanti, Alvia Rachma