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All Journal Journal Orthopaedi and Traumatology Surabaya (JOINTS) Folia Medica Indonesiana
Mubarok, Muhammad Iqbal
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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Optimal Dose of Vitamin D For Covid-19 Treatment Utami, Dita Mega; Ash-Siddiq, Muhammad Abdurrahman Rasyid; Rahmahani, Desi Rianti; Mubarok, Muhammad Iqbal; , Muhammad Zulkifly Tasman; Sibarani, Jeremy Nicolas; Fauziyah, Habibah Teniya Ariq; Utomo, Budi; Fauziyah, Shifa
Folia Medica Indonesiana Vol. 58, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: • Vitamin D supplementations in different doses yield different outcomes. • Multi-day vitamin D administration of 1000-6000 IU in patients with COVID-19 has more positive impacts than a single high dose of vitamin D. • Patient morbidity, length of hospitalization, and patient mortality improved with multi-day vitamin D administration. Abstract: This meta-analysis aimed to determine whether there is any optimal dose of vitamin D for morbidity, length of hospitalization, and mortality in patients with COVID-19. We conducted a comprehensive search in three online databases for eligible studies until February 28, 2022. Odds ratio (OR) and standardized mean difference (SMD) were applied as summary statistics of primary outcomes. The study quality of the literatures collected was assesed using the Cochrane risk of bias tool version 2 (RoB 2). Eight randomized clinical trials (RCT) were included in the study. In our analysis, we found that there was no significant difference in morbidity when vitamin D was administered to COVID-19 patients [OR=0.50 (95% CI=0.13-1.96); SMD=-0.14 (95% CI=-0.55-0.28)]. Duration of hospitalization [SMD=-0.12 (95% CI=-0.39-0.15)] and mortality [OR 0.47 (95% CI=0.19-1.17)] of COVID-19 patients in five studies also showed no significant difference compared to patients who do not take vitamin D. However, when we analyzed two other studies, we found that in patients who did not take vitamin D, mortality was lower [SMD=0.43 (95% CI=0.29, 0.58)]. Compared to a single high dose of vitamin D, the multi-day vitamin D administration of 1000-6000 IU in patients with COVID-19 resulted in improved patient morbidity, length of hospitalization, and patient mortality.
Hypoxic Culture (1% O2) Preserves Stemness and Reduces Spontaneous Osteogenic Differentiation In Early and Late Passage Bone Marrow-Derived Mesenchymal Stem Cells Rahadiansyah, Erreza; Mubarok, Muhammad Iqbal; Karsari, Deya
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 15 No. 1 (2026): April 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v15i1.2026.1-9

Abstract

Background: Bone marrow–derived mesenchymal stem cells (BMMSCs) are widely investigated for regenerative medicine applications. During in vitro expansion, BMMSCs may undergo loss of stemness and spontaneous lineage commitment. Hypoxic culture conditions better mimic the physiological bone marrow niche; however, their effects across early and late passages remain incompletely understood. This study aimed to evaluate the effect of hypoxic culture (1% O₂) on stemness-associated features, BMMSC identity, and spontaneous osteogenic mineralization at early and late passages.Methods: BMMSCs were isolated from a New Zealand White rabbit and cultured under normoxic (21% O₂) or hypoxic (1% O₂) conditions. Cells at passages 4 and 8 were analyzed for OCT4, SOX2, and CD105 expression using immunofluorescence and immunocytochemistry. Osteogenic differentiation was assessed by Alizarin Red S staining. Statistical analysis used independent t-tests and Mann–Whitney U tests (p < 0.05).Results: SOX2 and CD105 expression significantly decreased at late passages compared to early passages under both conditions (p < 0.05), whereas OCT4 expression remained stable under hypoxia (p > 0.05). BMMSCs cultured under hypoxia exhibited significantly higher expression of OCT4, SOX2, and CD105 than normoxic cultures at both passages (p < 0.05). Spontaneous calcium deposition was significantly lower under hypoxia (p < 0.05), with no significant difference between early and late passages.Conclusion: Hypoxic culture conditions better preserve stemness-associated features and MSC identity in BMMSCs and suppress spontaneous osteogenic mineralization. Prolonged hypoxic culture supports the maintenance of stem-like characteristics during in vitro expansion, highlighting its relevance for optimizing BMMSC culture strategies.
Co-Authors , Muhammad Zulkifly Tasman Ash-Siddiq, Muhammad Abdurrahman Rasyid Budi Utomo Deya Karsari, Deya Fauziyah, Habibah Teniya Ariq Rahadiansyah, Erreza Rahmahani, Desi Rianti Shifa Fauziyah Sibarani, Jeremy Nicolas Utami, Dita Mega