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All Journal Buletin Veteriner Udayana
Ni Luh Eka Setiasih
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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HISTOMORPHOMETRY OF SMALL INTESTINE OF BALI DUCKS AT DIFFERENT AGES Maria Nindi Dewi Sugiarto; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; I Ketut Suatha; Ni Nyoman Werdi Susari; I Gede Soma
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.34

Abstract

Bali ducks are one of the local poultry breeds that have high potential as producers of meat and eggs. The existence of this Balinese duck is spread evenly in rural areas in Bali. This study aims to histomorphometry of the small intestine of Bali ducks at different ages. In this study, 18 male Bali ducks were used as small intestine samples at 1 month, 3 months and 5 months. Bali ducks were obtained from a duck farm in Mengwi District, Badung Regency, Bali Province. Sampling of the small intestine was carried out in three parts, namely the duodenum, jejunum and ileum, then the organs were fixed with NBF 10% which was then made into histological preparations with HE staining. The research results are presented in a quantitative descriptive form. The results showed that the histological structure of the small intestine, namely the duodenum, jejunum and ileum, is composed of four layers, namely the tunica mucosa, tunica submucosa, tunica muscularis and the outermost is tunica serosa. The histomorphometric results, namely the total thickness of the layers that make up the small intestine of Bali ducks aged 1, 3, and 5 months respectively were 180.39 ± 29.59µm; 170.35±32.91µm; 302.09±78.94µm. It can be concluded that the histomorphometry of the small intestine of Bali ducks in ducks aged 1, 3, and 5 months has differences of thickness.
HISTOLOGIC FEATURES AND GRANULOCYTE WHITE BLOOD CELL COUNTS OF YOUNG AND ADULT SUMBAWA HORSES REARED UNDER THE LAR SYSTEM Angelica Ramadya Putri Candrianisa; Ni Ketut Suwiti; Tjokorda Sari Nindhia; Ni Luh Eka Setiasih; Anak Agung Sagung Kendran; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p21

Abstract

Leukocytes, are a crucial component of the immune system, protecting the body from infections such as bacteria, viruses, fungi, or parasites. This study aims to determine the histological structure of granulocyte white blood cells—basophils, eosinophils, and neutrophils—in young and adult Sumbawa horses. Blood samples were taken from 10 young and 10 adult Sumbawa horses, and histology preparations were made using eosin and methylene blue staining (MDT IndoReagen®). The histological examination and cell counts were conducted using an Olympus CX33 microscope, and data were analyzed with the T-test. The results showed that the histological structure of granulocyte leukocyte in both young and adult Sumbawa horses consisted of round cells with visible cytoplasm and nucleus. Neutrophils have 3-5 lobes with highly variable nucleus shapes and pale pink cytoplasm. The mean number of neutrophils in young Sumbawa horses was 34.20 ± 5.613%, while in adults it was 54.00 ± 6.324%. Eosinophils have a nucleus with two segmented lobes and very clear purplish-red granules in the cytoplasm. The number of eosinophils in young Sumbawa horses was 10.90 ± 2.726%, higher than in adults at 4.10 ± 1.595%. Basophils have very clear purplish-blue granules, with one nucleus filling almost the entire cytoplasm. The mean number of basophils in young Sumbawa horses was 0.20 ± 0.421%, and in adults, it was 0.10 ± 0.316%. In conclusion, the histological images of granulocyte leukocyte in Sumbawa horses show very clear granules, with the number of neutrophils young horses being lower than in adults and eosinophils being higher in young horses than in adults (P<0.05). However, there was no significant difference in the number of basophils (P>0.05). Further research is needed to compare the histology and agranulocyte white blood cell counts in Sumbawa horses to obtain more comprehensive information.
Co-Authors Anak Agung Sagung Kendran Angelica Ramadya Putri Candrianisa I Gede Soma I Ketut Suatha I Ketut Suatha Luh Gde Sri Surya Heryani Maria Nindi Dewi Sugiarto Ni Ketut Suwiti Ni Nyoman Werdi Susari Tjokorda Sari Nindhia