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All Journal Buletin Veteriner Udayana
I Ketut Suatha
Laboratorium Anatomi dan Embriologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, 80234, Indonesia
Immunology & microbiology Public Health Veterinary

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HISTOMORPHOMETRIC FEATURES DAN PERCENTAGE OF NEUTROPHILS AND BASOPHILS IN BALI CATTLE BASED ON CONVENTIONAL SYSTEM Angela Ivanka Benedicta; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; Ni Luh Eka Setiasih; I Ketut Suatha; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p24

Abstract

The Bali cattle rearing system in Bali is generally carried out using a conventional system. Conventional rearing is characterized by raising livestock on an area of land with food sources originating from the environment, so that the health of Bali cattle receives less attention. This maintenance method affects the body's resistance, one of the indicators is white blood cells. This study aims to determine the histology, morphometry and white blood cell count of neutrophils and basophils in Bali cattle based on conventional system. This research used samples in the form of blood from 30 Bali cattles, located in Bulian Village, Kubutambahan District, Buleleng Regency. Blood smears were fixed and stained using MDT IndoReagen®. Morphometric measurements of neutrophil and basophil white blood cells using the EP View application with 1000x magnification. Counting the number of neutrophils and basophils is done by counting from the edge of the field to the next edge, up to 100 leukocyte cells. The results showed that neutrophils have light pink, thin granular cytoplasm and have a segmented nucleus of 3-6 dark purple lobes with an average diameter of 5.79±0.57 µm. Basophils have purple granular cytoplasm and have a dark purple nucleus that is multi-segmented (polymorphonuclear) with an average diameter of 6.53±0.78 µm. The histological structure of neutrophils and basophils in Bali cattle based on conventional system is similar to neutrophils in other ruminants. The total number of neutrophil white blood cells was 14% and basophils was 8%. Further research needs to be done regarding health status by looking at other indicators such as the presence of white blood cells, eosinophils, lymphocytes and monocytes in Bali cattle based on conventional systems.
PRESERVATION OF CANINE FRONT AND HIND LIMB SPECIMENS USING PLASTINATION TECHNIQUE: IMPREGNATION PHASE IN A PASSIVE VACUUM CHAMBER Luh Gde Sri Surya Heryani; I Nengah Wandia; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p08

Abstract

The plastination technique is a preservation method that involves the infusion of polymer materials into biological specimens to maintain their structural integrity, prevent decay, and ensure long-term durability. This technique is widely regarded as an effective approach for preserving organs and is particularly valuable for anatomical education. The objective of this study is to evaluate the qualitative characteristics—specifically texture, color, and odor—of plastinated canine front and hind limb specimens during the impregnation phase, conducted within a passive vacuum chamber. Additionally, this research aims to propose recommendations for refining plastination techniques to enhance the quality of preserved organ specimens. The study employs a custom-designed apparatus and readily available generic chemicals to perform the plastination process. The plastination procedure consists of four key stages, with the impregnation phase being carried out in a vacuum chamber utilizing a passive vacuum system. The resulting plastinated specimens were assessed for flexibility, color, and odor. The findings revealed that the plastinated organs exhibited a rigid texture, a pale coloration, and a mild odor. The anticipated outcome of this research is to provide actionable recommendations for improving plastination methods, thereby enhancing the quality of plastinated organ products for use in veterinary anatomy education.
Co-Authors Anak Agung Sagung Kendran Angela Ivanka Benedicta I Nengah Wandia Luh Gde Sri Surya Heryani Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Nyoman Werdi Susari