Samsuri
Laboratorium Fisiologi, Farmakologi dan Farmasi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234

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HISTOPATHOLOGICAL PICTURE OF WHITE RATS HEART POST APPLICATION OF MIMOSIN FROM SIMPLISIA LAMTORO LEAF Ni Made Ayu Suastami; I Ketut Berata; Ni Luh Eka Setiasih; Samsuri; Luh Made Sudimartini; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p03

Abstract

Mimosine is a toxic compound that can be found in the leaves of the lamtoro plant (Leucaena leucocephala). Toxicity that occurs in livestock depends on the concentration of mimosine in the feed and the length of time it is administered. This study aims to determine the histopathological picture of the heart of white rats (Rattus norvegicus) given mimosine from lamtoro leaf simplicia at different doses. The research used 2 months old Wistar male white rats weighing 300-350 grams. The 20 experimental animals used were divided into 4 treatment groups, namely P0 (negative control) without mimosine, P1 (positive control) given standard mimosine 5 mg/head/day, P2 given lamtoro leaf simplicia at a dose of 50 mg/head/day, and P3 simplicia lamtoro leaves at a dose of 150 mg/head/day. Mimosine was given orally for 14 days using a probe. On the 15th day, a necropsy was performed, the heart organ was removed and placed in 10% NBF. Next, it is processed to make histopathological preparations with HE staining. Histopathological examination was carried out including three variables: congestion, bleeding and necrosis. The severity of the lesion was scored, namely 0, 1, 2, and 3 respectively for normal, mild, moderate, and severe lesions. The data was then analyzed using the non-parametric Kruskal-Wallis and Mann-Whitney tests. The results of the study showed that administration of mimosine from lamtoro leaf simplicia could cause histopathology in the form of congestion, bleeding and necrosis lesions compared to controls and there was no effect on the difference in the dose of mimosine from lamtoro leaf simplicia between doses of 50 mg/head/day and 150 mg/head/day.
DETERMINATION OF NON-SPECIFIC AND SPECIFIC QUALITY PARAMETERS OF MARIGOLD SIMPLICIA AS A RAW MATERIAL FOR MEDICINAL PREPARATIONS Martina; Samsuri; I Made Merdana; I Wayan Sudira; Putu Devi Jayanti
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p31

Abstract

Marigold flower (Tagetes erecta L.) is a type of ornamental plant from the Asteraceae family that has significant potential for cultivation due to its numerous uses. Besides being an ornamental flower, a natural dye, and an attractant for pest predators, marigold contains carotenoids, which can act as antioxidants, relieve mild fever and sore throat, serve as a natural moisturizer, and function as a natural mosquito repellent. Given its various beneficial components and properties, marigold has the potential to be used as a raw material for pharmaceutical preparations. This study aims to determine whether marigold flower simplicia meets the requirements as a raw material for pharmaceutical preparations based on non-specific and specific parameters according to established standards. The non-specific parameters include moisture content and ash content tests, while the specific parameters include organoleptic tests, water-soluble extract content, ethanol-soluble extract content, and yield percentage. This research is an observational non-experimental study with a descriptive-qualitative analysis design. Data collection was carried out by conducting non-specific and specific tests to obtain results for each parameter. The obtained results were as follows: moisture content 4.95%, ash content 4.8%, water-soluble extract content 37.34%, ethanol-soluble extract content 28%, and yield 13.48%. Based on these results, it can be concluded that the marigold flower simplicia is suitable and meets the requirements to be used as raw material for medicinal preparations, in accordance with the previously established parameters, namely the Materia Medica Indonesia Volume VI of 1995, the Ministry of Health of the Republic of Indonesia in 2017, and the Regulation of the Head of the Indonesian Food and Drug Authority (BPOM) Number 32 of 2019 concerning the quality requirements for traditional medicines. Further quality standardization tests should be conducted, such as microbial contamination, total aflatoxin, heavy metal contamination, weight uniformity, and alcohol content determination in marigold flower simplicia to establish additional quality standards across different parameters.