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All Journal Buletin Veteriner Udayana
Gede Putra Sanjaya
Laboratorium Bakteriologi dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Sanglah, Bali, Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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VARIATIONS OF COLIFORM BACTERIA ISOLATED FROM BALI CATTLE CALVES FECES, MARKETED AT BERINGKIT ANIMAL MARKET Egrina Sonta Bako; Hapsari Mahatmi; Putu Henrywaesa Sudipa; Gede Putra Sanjaya
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p11

Abstract

Beringkit Animal Market is one of the largest animal markets in Bali. Which is a place for livestock transactions, especially bali cattle calves, which are usually used as breeds. Bali cattle calves need digestive tract microbes such as bacteria to carry out the digestives process. Coliform bacteria are one of the bacteria that play an important role in the digestive tract of bali cattle calves. Coliform bacteria are one of the bacteria that plays an important role in the digestive tract of bali cattle calves. Coliform bacteria which are included in the Enterobacteriaceae group consist of Escherichia coli, Enterobacter sp., Klebsiella sp., and Citrobacter sp. This research aims to determine what variations of Coliform bacteria are found in the feces of bali cattle calves marketed at the Beringkit Animal Market. This research was observational with the number of samples used were 50 fecal samples from 50 bali cattle calves. The samples were isolated on Eosin Methylene Blue Agar (EMBA) media and then tested and identified using the Indole Methyl Voges Proskauer Citrate (IMViC) principles and Blood agar test. The result showed that 82% of samples were positive Coliform. Based on the result, it can be concluded that in the feces of bali cattle calves marketed at the Pasar Hewan Beringkit contain Coliform bacteria consisting of Escherichia coli, Enterobacter sp., Klebsiella sp., and Citrobacter sp. with the highest percentage was Escherichia coli at 58%, followed by Citrobacter sp. at 12% and Enterobacter sp. and klebsiella sp. respectively 6%. It is necessary to count the total number of each bacteria to determine the pathogenicity of the bacteria.
CONTAMINATION SALMONELLA SPP. IN BROILER CHICKEN MEAT SOLD IN THE BADUNG MARKET DURING THE TRANSITIONAL SEASON PERIOD Nabila Dwifitria Azis; Hapsari Mahatmi; I Wayan Suardana; Gede Putra Sanjaya
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p10

Abstract

The presence of Salmonella spp. in animal food products is highly undesirable and strictly regulated in Indonesian law. One of the factors that causes contamination is the climate and conditions of the place of sale. This study aims to determine the presence of Salmonella spp. bacteria in broiler meat that sold at Badung Market during the transitional season period. The research sample was fresh broiler chicken meat, namely 15 samples taken randomly from 15 broiler chicken traders. The isolation stage begins with enrichment using Selenite broth media, followed by isolation of bacteria using selective media, namely Salmonella Shigella Agar (SSA), identification using the Gram staining method, and Biochemical Tests including the Triple Sugar Iron Agar Test, Methyl Red, Indole, and Citrate tests. The results of the research found that 5 of 15 (33,3%) samples were identified as contaminated with Salmonella spp. Based on the research results, it can be concluded that broiler chicken meat sold in the Badung market during the transitional season is of poor quality. It is hoped that the government will pay more attention and provide education regarding the procedures and procedures for selling chicken meat according to standards and implement the applicable regulations more strictly, because this can have a serious impact on public health.
ESCHERICHIA COLI CONTAMINATION ON IMPORTED FROZEN BUFFALO MEAT SOLD AT AIKMEL TRADITIONAL MARKET, AIKMEL DISTRICT, EAST LOMBOK DISTRICT, WEST NUSA TENGGARA PROVINCE Rahmat Ade Verdiasyah; Hapsari Mahatmi; I Made Sukada; Gede Putra Sanjaya
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p24

Abstract

The demand for beef in Indonesia continues to increase. The government is striving to achieve self-sufficiency in beef production, but the high price of beef is driving imports of buffalo meat from India. Buffalo meat has high nutritional value, similar to beef. Since 2015, frozen buffalo meat imports from India have begun to enter Indonesia, particularly in West Nusa Tenggara, as an effort to meet market demand in the modern shopping areas in Mataram City, Lombok. The relatively cheap price of frozen buffalo meat has led to expanded marketing in East Lombok, especially in the traditional market of Aikmel, which is approximately 72 km away from Mataram. The aim of this research is to determine the extent of Escherichia coli contamination and the amount of Escherichia coli contamination per gram of buffalo meat sold in this traditional market. The research samples consist of frozen buffalo meat from India sourced from 10 Indian buffalo meat vendors in the Aikmel market, each weighing 100 grams. The isolation and identification method follows Jawetz et al. (2008), starting with isolation on Eosin Methylene Blue Agar (EMBA), followed by identification with INMVIC (Indole, Methyl Red, Voges Proskauer, Citrate), reinforced by results from TSIA (Triple Sugar Iron Agar) and Urease tests. The research found that E. coli contamination exceeded the specified limit (SNI 7388:2009, 2009) in 9 out of 10 samples taken from 10 Indian buffalo meat vendors in the Aikmel market. The smallest and largest amounts of E. coli contamination in 90% of the contaminated samples were 80x101 and 7x103 colonies/gram, respectively. This condition is crucial for the government to pay attention to, in order to enhance education and supervision of the public regarding the procedures and regulations for the sale of frozen buffalo meat from India, in accordance with the marketing of animal origin products.
CONTAMINATION ESCHERICHIA COLI IN BROILER CHICKEN MEAT SOLD AT BADUNG MARKET, DENPASAR, BALI DURING THE TRANSITION SEASON Riki Jainudin; Hapsari Mahatmi; Romy Muhammad Dary Mufa; Gede Putra Sanjaya
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i3.p12

Abstract

Escherichia coli is reported as an intensive bacterial contaminant of chicken meat, including broiler meat. Factors that support the occurrence of contamination are the environment, equipment and sellers. This study aims to determine the presence of Escherichia coli bacteria in broiler meat sold in Badung market, Denpasar, Bali during the transitional season. The samples used were fresh broiler meat totaling 15 samples by taking randomly from 15 broiler meat traders. Samples will be taken to the laboratory for isolation and identification of Escherichia coli. The isolation stage uses selective media, namely Eosin Methylen Blue Agar (EMBA), identifying with the gram staining method, and biochemical tests consisting of Triple Sugar Iron Agar (TSIA), Citrate, Indol and Methyl Red tests. The results of the study found 3 (20%) of the 15 samples identified were contaminated by Escherichia coli bacteria. From the results of this study there is still contamination of Escherichia coli bacteria and needs to get more attention from the government in order to get education about the procedures for selling broiler meat according to standards and implement applicable regulations more strictly, because it affects public health.
Co-Authors Egrina Sonta Bako Hapsari Mahatmi I Made Sukada I Wayan Suardana Nabila Dwifitria Azis Putu Henrywaesa Sudipa Rahmat Ade Verdiasyah Riki Jainudin Romy Muhammad Dary Mufa