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All Journal Buletin Veteriner Udayana
Hamong Suharsono
Laboratorium Biokimia Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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CHARACTERISATION OF ADHESIN AND RECEPTOR MOLECULES IN THE GASTRIC EPITHELIUM OF MICE: PATHOGENESIS STUDY AND DEVELOPMENT OF DIAGNOSTIC MATERIALS FOR HELICOBACTER PYLORI INFECTION Hamong Suharsono
Buletin Veteriner Udayana Bul. Vet. Udayana. October 2024 Vol. 16 No. 5
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i05.p16

Abstract

This research focuses on the characterization of adhesin and receptor molecules on the gastric epithelium of mice to understand the pathogenesis mechanisms of Helicobacter pylori infection and its potential in the development of diagnostic materials. Infection by H. pylori begins with the colonization process on the surface of the gastric mucosa, where the bacteria use various attachment factors, including pili and outer membrane proteins (OMPs), to adhere to host cells. This study involves the isolation of H. pylori pili using the pili cutter method and the isolation of hemagglutinin protein from OMPs through centrifugation and dialysis processes. These proteins were then analyzed through hemagglutination assays, SDS-PAGE, Western blotting, as well as adhesion and immunohistochemistry tests to evaluate the specific interactions between H. pylori adhesins and gastric epithelial cells. The results identified adhesins such as BabA and SabA, which play a crucial role in H. pylori adhesion to the gastric epithelium, particularly by binding to Lewis blood group antigens. Additionally, it is suspected that there are other adhesin proteins that may originate from the tip of H. pylori pili associated with the type IV secretion system (T4SS). Hemagglutination assays demonstrated significant hemagglutination activity in the adhesin proteins, while Western blotting results showed specific interactions between the adhesin proteins and antibodies. Furthermore, adhesion and immunohistochemistry tests confirmed the ability of H. pylori adhesins to adhere to mouse gastric epithelial cells. These findings enhance our understanding of the role of adhesins in the pathogenesis of H. pylori infection, particularly during the early stages of colonization, and open up opportunities for the development of diagnostic materials that can detect the presence of H. pylori based on adhesin-receptor interactions. This research provides a scientific foundation for the development of new strategies in the diagnosis and management of H. pylori infection.
THE LEVEL OF KNOWLEDGE AND APPLICATION OF ANIMAL WELFARE PRINCIPLES BY K-9 HANDLERS AT THE DIRECTORATE OF ANIMAL POLICE KORSABHARA BAHARKAM POLRI Gabriella Panyuwa; Hamong Suharsono; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p21

Abstract

K-9 handlers at the Directorate of Animal Police, Korsabhara Baharkam Polri, play a crucial role in ensuring the welfare and adequate knowledge related to the K-9s under their care. The importance of surveying the level of knowledge and the implementation of animal welfare principles by K-9 handlers becomes evident to ensure that these animals' lives adhere to ethical and welfare standards. The aims of this study is to assess the level of knowledge of K-9 handlers at the Directorate of Animal Police, Korsabhara Baharkam Polri, regarding animal welfare principles and to evaluate the extent to which these principles are applied. The method employed is a survey involving 40 respondents, consisting of 10 K-9 handlers from each of the four different units: general tracking, explosives, search and rescue (SAR), and narcotics. The collected data were analyzed qualitatively using a descriptive approach and presented as percentages in tables. The results indicate that the level of knowledge of K-9 handlers regarding animal welfare is classified as good, with an average score of 73.59%. Meanwhile, the implementation of animal welfare principles reaches an average of 90.05%, with the highest implementation in the aspect of freedom from fear and distress (96%) and the lowest in the aspect of freedom from discomfort (79.6%). The level of knowledge and application of animal welfare in the aspect of freedom from discomfort needs to be further improved.
ANTIBODY RESPONSE IN MICE IMMUNIZED WITH ASF_703 DNA VACCINE Fidella Luthfia Qotrunnada; I Gusti Ngurah Kade Mahardika; Hamong Suharsono
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p03

Abstract

African Swine Fever (ASF) is a highly contagious viral disease in pigs, causing significant economic losses due to high mortality rate and the lack of an effective vaccine. On going vaccine development efforts include research at the Biomedical Laboratory of the Faculty of Veterinary Medicine, Udayana University, which previously developed the ASF_701 DNA vaccine. The study aimed to evaluate the immune response in mice to a novel ASD DNA vaccine formulation containing four additional genes (EP153R, A238L, DP96R, and S276R) and a polymer adjuvant. In this experimental study, mice were divided into two groups: a control group and a vaccinated group. Each mouse in the vaccinated group received a subcutaneous injection of 25 µg plasmid DNA in polymer adjuvant. The immune response was assessed using ELISA to detect ASF-specific antibodies at weeks 0, 1, 2, 3, and 4, with synthetic peptides as coating antigens. Optical Density (OD) values were statistically analyxed using SPSS version 25. The results demonstrated a significant difference (p < 0.01) in antibody levels between vaccinated and control mice, with serum collection time also showing a significant effect (p = 0.004). The finfings suggest that the new vaccine formulation elicits a measurable immune response. However, further studies are needed to improve the stability and long-term efficacy of the vaccine.
PERFORMANCE OF BROILER CHICKEN WITH ADDITION OF MAGGOT FLOUR IN FEED Irindra Sekar Jalu; Hamong Suharsono; I Ketut Sumadi
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p15

Abstract

This study aimed to determine the effect of adding Black Soldier Fly (Hermetia illucens) maggot meal to commercial feed on the growth performance of broilers (Gallus domesticus), particularly body weight gain and feed conversion ratio (FCR). The research was conducted using a Completely Randomized Design (CRD) with four treatments: P0 (control), P1, P2, and P3, each with six replications. Broilers were raised from Day Old Chick (DOC) and fed BR I Crumbel feed from day 1 to 21 and BR II SP-Pellet from day 22 to 34. The maggot meal supplementation was given from day 14 to day 34. The results showed that the addition of Hermetia illucens maggot meal to commercial feed had no significant effect (P>0.05) on the broilers’ body weight gain or feed conversion ratio. Therefore, under the conditions of this study, the inclusion of maggot meal in the diet did not significantly improve the growth performance of broilers.
Co-Authors Fidella Luthfia Qotrunnada Gabriella Panyuwa I Gusti Ngurah Kade Mahardika I Ketut Sumadi Irindra Sekar Jalu Romy Muhammad Dary Mufa