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All Journal Buletin Veteriner Udayana
Ida Bagus Komang Ardana
Laboratorium Patologi Klinik Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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THE EFFECT OF NATURAL GUARD IN DRINKING WATER ON NEWCASTLE DISEASE ANTIBODY TITER IN LAYING HENS Misfah Maulidiyah; Ida Bagus Komang Ardana; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p17

Abstract

Natural Guard is a combination of pine oil, lavender oil and eucalyptus oil which can be used as an immunomodulator. The aim of this study was to determine the effect of Natural Guard administration through drinking water on the antibody titer against Newcastle Disease (ND) virus. This study used 24 Hy-line Brown laying hens randomly assigned to four treatment groups. Natural Guard was mixed into the drinking water at different concentrations: 0 ml/L (control), 0.1 ml/L (P1), 0.2 ml/L (P2), and 0.3 ml/L (P3), and administered for 30 days. ND antibody titers were measured using the Hemagglutination Inhibition (HI) test. The data obtained were analyzed using analysis of variance. The results showed that administration of Natural Guard at doses of 0.1 ml/L, 0.2 ml/L, and 0.3 ml/L did not significantly (P > 0.05) increase ND virus antibody titers in laying hens.
THE EFFECT OF NATURAL GUARD SUPPLEMENT IN BROILER DRINKING WATER ON THE HEALTH AND SAFETY OF THE LIVER, SPLEEN, AND KIDNEYS Millaniladzim Baadilla; Ida Bagus Komang Ardana; I Ketut Eli Supartika; I Made Merdana Merdana; Luh Made Sudimartini
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p18

Abstract

The broiler industry in Indonesia has grown rapidly since the 1970s and has become a primary source of animal protein. However, the health of vital organs such as the liver, spleen, and kidneys plays a crucial role in broiler productivity. Natural Guard (NG) is a feed supplement claimed to benefit these organs, but research on its safety remains limited. This study aimed to evaluate the effect of NG supplementation through drinking water on the health of the liver, spleen, and kidneys in broilers. A completely randomized design (CRD) was used, with four treatment groups: control without NG (P0) and NG supplementation at 1 g/L (P1), 2 g/L (P2), and 4 g/L (P3). Histopathological analysis was performed using Hematoxylin-Eosin (HE) staining, and data were analyzed using the Kruskal-Wallis test. The results showed no necrotic lesions in any group, while congestion and inflammatory cell infiltration were observed at mild to moderate levels without significant differences (P>0.05). This indicates that NG supplementation up to 4 g/L does not cause harmful pathological changes in the liver, spleen, and kidneys of broilers. In conclusion, NG is safe to use as a feed supplement for broilers. Further research is needed to assess its long-term effects and impact on broiler productivity.
Co-Authors I Ketut Eli Supartika I Made Merdana I Made Merdana Merdana Ida Bagus Kade Suardana Luh Made Sudimartini Millaniladzim Baadilla Misfah Maulidiyah