Article Per Year (5 Year)
p-Index From 2021 - 2026
1.285
P-Index
This Author published in this journals
All Journal Buletin Veteriner Udayana
Tri Komala Sari
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
ESCHERICHIA COLI AND SHIGELLA SP. INFECTIONS IN BROILER CHICKENS AT A CLOSED HOUSE FARM IN BATUNGSEL VILLAGE, TABANAN I Putu Gde Surya Nagara Ardiana; Hapsari Mahatmi; I Ketut Berata; Nyoman Adi Suratma; Tri Komala Sari
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2024 Vol. 16 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i06.p20

Abstract

Escherichia coli is a coliform bacterium naturally found in the intestines of mammals. However, pathogenic strains, such as Avian Pathogenic Escherichia coli (APEC), can cause systemic infections and bacteremia in poultry. Infections by Escherichia coli in broilers lead to economic losses due to decreased production and increased mortality. This case report was conducted under protocol number 1/N/24, using anamnesis, clinical signs, epidemiological data, anatomical pathology, and histopathology observations to diagnose the condition. A 28-day-old white broiler chicken was collected from a closed house farm in Batungsel Village, Pupuan District, Tabanan Regency. Observed signs included lethargy, reduced appetite, an enlarged reddish abdomen, and white diarrhea. After the chicken's death, a necropsy was performed, and organ samples were preserved in 10% Neutral Buffered Formalin (NBF). Samples of the brain, lungs, liver, heart, spleen, kidneys, intestines, bursa, and feces were analyzed in histopathology, bacteriology, and parasitology laboratories. Histopathological preparations were stained with hematoxylin and eosin for microscopic examination. Bacterial infection tests included culturing samples from the intestines, liver, lungs, and heart on general, selective-differential, and Blood Agar media, followed by primary and secondary tests. The presence of Escherichia coli and Shigella sp. was confirmed. Parasite examinations using the flotation method showed no worm eggs or coccidia. These findings confirmed that the chicken was infected with Escherichia coli and Shigella sp.To prevent such infections, maintaining clean and sanitized housing is essential. Strict biosecurity measures are crucial to prevent external bacterial contamination. With good management practices, broiler chicken health can be optimally maintained.
CASE OF COLISEPTICEMIA IN BROILER CHICKEN AT CLOSED HOUSE FARM IN JIMBARAN, BADUNG REGENCY, BALI Alfaine Nova Chumayroh; I Gusti Ketut Suarjana; I Ketut Berata; Tri Komala Sari; Ida Bagus Made Oka
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p01

Abstract

Avian Pathogenic Escherichia coli (APEC) is a pathogenic strain of E. coli that can cause systemic infections in poultry, leading to colisepticemia in chickens. This condition reduces productivity and increases financial losses for farmers. This case study aims to obtain a definitive diagnosis of the disease affecting 21-day-old broiler chickens in a farm located in Jimbaran, South Kuta District, Badung Regency, Bali. The methods used include clinical symptom observation, epidemiological analysis, anatomical pathology, histopathology, and laboratory examinations for bacteriology and parasitology. The observed symptoms included weakness, decreased appetite and water intake, dull and stiff feathers, diarrhea, and the presence of solidified yellowish-white feces around the cloaca. Epidemiological analysis revealed that the farm had approximately ±10,000 chickens, with around ±1,800 chickens exhibiting similar symptoms and approximately ±500 deaths. The morbidity rate was 18%, the mortality rate was 5%, and the case fatality rate (CFR) was 27.8%. Gross pathology examination showed the presence of fibrin in the abdominal and thoracic areas, covering the heart, liver, lungs, and kidneys. Histopathological examination revealed inflammation accompanied by heterophil inflammatory cell infiltration, necrosis, edema, hemorrhage, and congestion. Bacteriological examination was conducted by isolating bacteria using general and selective media, supported by Gram staining, catalase test, biochemical tests, and glucose fermentation tests, confirming the presence of Escherichia coli in the heart, liver, lungs, and intestines. Parasitological examination using native, sedimentation, and flotation methods on fecal samples showed no parasitic infection. The study concludes that the chickens were diagnosed with colisepticemia without any parasitic infection. Prevention measures should focus on maintaining biosecurity and farm sanitation to minimize infections caused by various pathogenic agents.
PROTECTIVE EFFECTS OF SEA GRAPE SIMPLICIA ON LUNG HISTOPATHOLOGY IN PARACETAMOL-INDUCED RATS Reyna Tasya Dhewanty; I Ketut Berata; Tri Komala Sari; Samsuri; I Made Merdana
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p10

Abstract

Caulerpa lentillifera, commonly known as sea grapes, is a green alga found in shallow sandy areas and around coral reefs. It contains chlorophyll A and B, polyphenols, flavonoids, tannins, and vitamins A, B1, B2, B6, B12, and C—all of which possess antioxidant properties. Antioxidants play a critical role in preventing and repairing cellular damage. This study aimed to evaluate the protective effects of C. lentillifera simplicia on the lung histopathology of white rats (Rattus norvegicus) induced with paracetamol. Twenty-five male rats were randomly assigned to five groups and treated over a 14-day period. Lung tissues were collected on day 15 for histopathological analysis, focusing on indicators such as hemorrhage, inflammation, congestion, and necrosis. Data were analyzed using the Kruskal–Wallis and Mann–Whitney tests. Paracetamol administration caused significant histopathological damage to lung tissues. However, rats treated with C. lentillifera simplicia exhibited notable improvements, with reduced severity of hemorrhage, inflammation, congestion, and necrosis compared to the paracetamol-only group. These findings indicate that C. lentillifera simplicia has a protective effect against paracetamol-induced lung injury, supporting its potential use as a natural antioxidant for lung tissue protection.
CO-INFECTION OF AVIAN INFLUENZA H5N1 AND COCCIDIOSIS IN A 25-DAY-OLD BROILER: A CASE REPORT Indira Laksmi Nandita; Tri Komala Sari; Ida Bagus Oka Winaya; Yeocelin Meida Utami; Ni Luh Putu Diah Septianingsih
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p14

Abstract

Avian Influenza (AI) is a highly pathogenic contagious disease in poultry caused by influenza A virus, particularly the H5N1 subtype. This disease significantly affects poultry health and causes considerable economic losses in poultry farming. This case report describes a 25-day-old broiler chicken from Demulih Village, Bangli, Bali, which showed symptoms of anorexia, weakness, greenish diarrhea, and rapid death. Epidemiological analysis revealed a morbidity rate of 33.3%, mortality rate of 22.7%, and case fatality rate of 68.3%. Anatomical pathology examination showed multiorgan hemorrhages, while histopathology revealed non-suppurative inflammation with lymphocytic infiltration in the brain, lungs, heart, pancreas, and intestine. Virus isolation using embryonated chicken eggs, along with hemagglutination (HA) and hemagglutination inhibition (HI) tests, confirmed Avian Influenza H5N1 infection. In addition, fecal examination identified Eimeria spp. oocysts, indicating coccidiosis. These findings highlight that H5N1 infection can be aggravated by parasitic co-infections, leading to increased mortality. Preventive measures should include routine vaccination, strict biosecurity, and good husbandry management to reduce the risk of disease spread in the future.
Co-Authors Alfaine Nova Chumayroh Hapsari Mahatmi I Gusti Ketut Suarjana I Ketut Berata I Made Merdana I Putu Gde Surya Nagara Ardiana Ida Bagus Made Oka Ida Bagus Oka Winaya Indira Laksmi Nandita Ni Luh Putu Diah Septianingsih Nyoman Adi Suratma Reyna Tasya Dhewanty Samsuri Yeocelin Meida Utami