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All Journal Buletin Veteriner Udayana
Ida Bagus Oka Winaya
Laboratorium Patologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Sanglah, Denpasar, Bali; Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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CASE STUDY OF NEWCASTLE DISEASE VELOGENIC STRAIN WITH MULTIORGAN LESSIONS IN VACCINATED BROILER IN SUSUT, BANGLI I Gde Andhika Putra Pratama; I Gusti Ngurah Kade Mahardika; Ida Bagus Oka Winaya; I Nengah Kerta Besung; Ida Bagus Made Oka
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p16

Abstract

Newcastle disease (ND) is a highly contagious viral disease of poultry with high mortality rates in velogenic strains. This paper present findings about velogenic strain of Newcastle disease in case study. The methods used in examination of animal case are observation, anatomical pathology examination, histopathology examination, TAB cultivation, serological tests, bacterial identification and parasite identification. In this case study, 28-day-old broiler chickens were obtained from a farmer in Demulih Village, Susut, Bangli. The clinical symptoms observed in the affected chickens included weakness, anorexia, difficulty breathing, greenish-white diarrhea, and torticollis. Pathological anatomical examination revealed congestion in the brain, hemorrhages in the trachea, lungs, heart, and intestines, as well as petechiae in the proventriculus. Histopathological examination showed inflammatory changes were dominated marked by mononuclear cell infiltration in the brain, trachea, lung, heart, proventriculus and intestine. Result of TAB cultivation continued with using HA test showed that virus can agglutination erythrocytes with titer 210 HA Unit. ND virus was confirmed by HI test. Based on this case study, it was concluded that the chickens were infected with ND.
EFFECTS OF CAULERPA LENTILLIFERA SIMPLISIA ADMINISTRATION ON GASTRIC AND SMALL INTESTINAL HISTOPATHOLOGY IN WHITE RATS POST PARACETAMOL INDUCTION Baiq Melisa Ulandari Utami; Ida Bagus Oka Winaya; Yudha Yaksa Crada Yoga Arum Raharjo; Samsuri; I Made Merdana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p04

Abstract

Caulerpa lentillifera is known as sea grapes macroalgae or sea grapes which have benefits as antioxidants. Paracetamol is a drug that is sold freely and many people abuse it. Abuse of paracetamol can cause negative impacts on various organs, one of which is the stomach and small intestine. The purpose of this study was to determine the effect of administering Caulerpa lentillifera on the histopathology of the gastric and small intestine of white mice given paracetamol in high doses. This study used a completely randomized design method. A sample of 25 male white mice was divided into 5 treatment groups, namely negative control (P0) or no treatment, positive control (P1) was only given paracetamol 250 mg/kgBB, P2 was given paracetamol 250 mg/kgBB and sea grapes 10 g/kgBB, P3 was given paracetamol 250 mg/kgBB and sea grapes 15 g/kgBB, and P4 was given paracetamol 250 mg/kgBB and sea grapes 20 g/kgBB. Treatment was given for 14 days, then necropsied. Samples of gastric and small intestine tissue were made into histopathology preparations using hematoxylin eosin (HE) staining. The variables examined in the gastric were gastric mucosal damage. While the variables examined in the small intestine included bleeding lesions and necrosis. Data were analyzed using the Kruskal Wallis Test followed by the Mann-Whitney test and described descriptively. The results showed that in gastric mucosal damage, small intestine necrosis, and small intestine bleeding showed that there was a difference (P<0.05) between groups. There was a process of improvement in the histopathology of the gastric and small intestine of white rats given Caulerpa lentillifera in gastric mucosal damage, small intestine necrosis, and small intestine bleeding. Based on the results of the study, it can be concluded that sea grapes with doses of 15 g/kgBB and 20 g/kgBB can minimize gastric mucosal damage. While a dose of 20 g/kgBB can minimize small intestine damage in necrosis and hemorrhage lesions
HISTOPATHOLOGICAL ANALYSIS OF THE PANCREAS IN WHITE RATS FOLLOWING ADMINISTRATION OF PURE MIMOSINE AND MIMOSINE IN LAMTORO LEAF POWDER I Ketut Berata; I Made Kardena; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p03

Abstract

Mimosine in the lamtoro plant (Leucaena leucochepala) is a toxic or anti-nutritional ingredient which is reported to mainly cause hair loss in cattle. In addition, there is the possibility of many impacts on body tissue as a result of consuming feed ingredients containing mimosine. This study aims to determine the impact of mimosine in lamtoro leaf powder on histopathological changes in the pancreas of white rats (Rattus norvegicus). A total of 18 male Wistar strain white rats, 2 months old with a body weight of 250-300 g, were used in this study. Rats were randomly divided into 3 treatment groups, namely group P0 as a negative control (placebo), group P1 as a positive control given pure mimosine produced by Sigma at a dose of 5 mg/day, group P2 given mimosine from lamtoro leaf powder as a positive control at a dose of 50 mg/day Treatment was carried out every day for 14 days with a sonde. On day 15, all mice were euthanized and sacrificed for their pancreatic tissue. Next, the pancreas is processed to make histopathological preparations using the hematoxylin eosin (HE) staining method. Histopathological changes examined included lesions of congestion, bleeding and necrosis. Examination data is scored according to the distribution of lesions, namely normal criteria (score 0) if there are no lesions, mild criteria (score 1) if the lesion is focal, moderate criteria (score 2) if the lesion is multifocal and severe criteria (score 3) if the lesion is diffuse. The results of the examination showed that moderate levels of congestion lesions were found when mimosine was administered in lamtoro leaf powder. Mild necrotic lesions were found when mimosine was administered in lamtoro leaf powder, while moderate levels of necrotic lesions were found when standard mimosine was administered. It can be concluded that mimosine in lamtoro leaf powder can cause milder necrosis in the pancreas of white rats than standard mimosine administration. The flavonoid, tannin and protein components in lamtoro leaf powder can reduce the toxicity of mimosine. To reduce the effects of mimosine poisoning, it is best to limit the feeding of lamtoro leaves to livestock or before giving them to livestock, they should be given treatment to reduce the toxic effects.
FATAL CANINE PARVOVIRUS INFECTION WITH SECONDARY BACTERIAL AND PARASITIC COMPLICATIONS IN A PUPPY: A CASE REPORT Egrina Sonta Bako; I Putu Cahyadi Putra; Ida Bagus Oka Winaya; I Gusti Ngurah Kade Mahardika; I Nengah Kerta Besung
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p13

Abstract

Canine parvovirus (CPV) is a highly infectious viral disease that poses a significant threat to puppies, often resulting in fatality if not diagnosed and treated promptly. This report investigates the cause of death of a nearly two-month-old puppy in Denpasar City. Diagnosis was confirmed through a comprehensive approach, including a thorough history, clinical examination, hematology, anatomical pathology, histopathology with hematoxylin and eosin (H&E) staining, polymerase chain reaction (PCR) for CPV, bacteriology, and parasitology examinations. Anamnesis revealed that the two puppies succumbed after exhibiting symptoms such as anorexia, vomiting, bloody diarrhea, dehydration, and cachexia, which were also observed in this puppy. Necropsy findings indicated multiorgan lesions, including hemorrhage, congestion, hyperemia, uneven organ discoloration, swelling of the heart and spleen, and worm infestation in the stomach. Histopathological analysis revealed multi-organ inflammation characterized by hemorrhage, necrosis, edema, and inflammatory cell infiltration in all organs, with lesions typical of CPV, including lymphoid follicular necrosis and intestinal Lieberkühn crypts in the intestine. PCR confirmed CPV infection, as evidenced by a 900 bp DNA band. Bacteriological examination revealed the presence of Escherichia coli and Streptococcus spp. in the intestine and Staphylococcus spp. in the spleen. Parasitological examination revealed the presence of adult worms, larvae, and eggs of Toxocara canis in the fecal sample. Based on these findings, the death of the puppy was attributed to CPV infection compounded by secondary bacterial and parasitic infections. Further testing is recommended to ascertain potential co-infection with Canine Distemper Virus.
COINFECTION OF NEWCASTLE DISEASE AND CAPILLARIA SPP. IN LOCAL CHICKENS IN KERTA VILLAGE, PAYANGAN DISTRICT, GIANYAR REGENCY, BALI Ketut Nanda Maharanthi; Ida Bagus Kade Suardana; I Putu Cahyadi Putra; Ni Wayan Helpina Widyasanti; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p28

Abstract

Newcastle Disease (ND) is a viral infection that significantly affects poultry and leads to substantial economic losses in the poultry industry. This case study aimed to achieve a definitive diagnosis of the disease in a seven-month-old free-range chicken owned by a resident of Kerta Village, Payangan Subdistrict, Gianyar Regency, Bali. The methodologies employed encompassed the collection of clinical and epidemiological data through history taking, anatomical pathology examination, histopathology, bacteriology, parasitology, and virus isolation via culture of embryonated chicken eggs, which were subsequently confirmed using hemagglutination (HA) and hemagglutination inhibition (HI) tests. Tracing and history-taking revealed that the chicken rearing system was semi-intensive. The chicken population comprised 15 chickens, of which 12 exhibited illness and 10 succumbed within eight days. The clinical signs observed included lethargy, pale bluish drooping wattle and cockscomb, periorbital edema, respiratory distress, reduced appetite and water intake, greenish diarrhea, torticollis, and limp drooping wings. Epidemiological data indicate a morbidity rate of 80%, a mortality rate of 67%, and a case fatality rate of 83%. Anatomical pathological examination through necropsy revealed multiorgan hemorrhage, pulmonary hemorrhage, petechiae in the proventriculus, and intestinal hemorrhage. Organ samples were collected for histopathological analysis with hematoxylin-eosin (HE) staining, which revealed inflammation in various organs, predominantly characterized by lymphocyte infiltration. The HA test demonstrated a high viral titer (29), and the HI test confirmed a positive ND virus infection. Bacteriological examination revealed Escherichia coli in the small intestine and cecum as part of the normal flora. Fecal examination revealed 100 eggs per gram of Capillaria spp. Based on these results, the chickens were diagnosed with a coinfection of Newcastle Disease virus and Capillaria spp. The implementation of routine vaccination programs, improved management practices, and control of poultry movement is recommended as disease prevention measures.
CO-INFECTION OF AVIAN INFLUENZA H5N1 AND COCCIDIOSIS IN A 25-DAY-OLD BROILER: A CASE REPORT Indira Laksmi Nandita; Tri Komala Sari; Ida Bagus Oka Winaya; Yeocelin Meida Utami; Ni Luh Putu Diah Septianingsih
Buletin Veteriner Udayana Bul. Vet. Udayana. December 2025 Vol. 17 No. 6
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i06.p14

Abstract

Avian Influenza (AI) is a highly pathogenic contagious disease in poultry caused by influenza A virus, particularly the H5N1 subtype. This disease significantly affects poultry health and causes considerable economic losses in poultry farming. This case report describes a 25-day-old broiler chicken from Demulih Village, Bangli, Bali, which showed symptoms of anorexia, weakness, greenish diarrhea, and rapid death. Epidemiological analysis revealed a morbidity rate of 33.3%, mortality rate of 22.7%, and case fatality rate of 68.3%. Anatomical pathology examination showed multiorgan hemorrhages, while histopathology revealed non-suppurative inflammation with lymphocytic infiltration in the brain, lungs, heart, pancreas, and intestine. Virus isolation using embryonated chicken eggs, along with hemagglutination (HA) and hemagglutination inhibition (HI) tests, confirmed Avian Influenza H5N1 infection. In addition, fecal examination identified Eimeria spp. oocysts, indicating coccidiosis. These findings highlight that H5N1 infection can be aggravated by parasitic co-infections, leading to increased mortality. Preventive measures should include routine vaccination, strict biosecurity, and good husbandry management to reduce the risk of disease spread in the future.
Co-Authors Baiq Melisa Ulandari Utami Egrina Sonta Bako I Gde Andhika Putra Pratama I Gusti Ngurah Kade Mahardika I Ketut Berata I Made Kardena I Made Merdana I Nengah Kerta Besung I Nengah Kerta Besung I Putu Cahyadi Putra I Putu Cahyadi Putra, I Putu Cahyadi Ida Bagus Kade Suardana Ida Bagus Made Oka Indira Laksmi Nandita Ketut Nanda Maharanthi Ni Luh Putu Diah Septianingsih Ni Wayan Helpina Widyasanti Samsuri Tri Komala Sari Yeocelin Meida Utami Yudha Yaksa Crada Yoga Arum Raharjo