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All Journal Buletin Veteriner Udayana
Ni Luh Eka Setiasih
Laboratorium Histologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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HISTOLOGIC FEATURES OF GRANULOCYTE WHITE BLOOD CELLS AND PLATELET DISTRIBUTION WIDTH VALUES IN DOGS WITH DERMATITIS Patricia Amelia Debianti Wagut; Ni Ketut Suwiti; Sri Kayati Widyastuti; Nyoman Sadra Dharmawan; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p35

Abstract

Dermatitis in dogs is an inflammation of the skin caused by parasites, bacteria, fungi and metabolic disorders, with severity varying from mild to severe. This condition triggers inflammation that affects granulocyte leukocytes, namely neutrophils, eosinophils and basophils, which can undergo necrosis. Necrosis is characterized by changes in nuclear morphology, such as pycnosis, karyorexis, and karyolysis due to irreversible cell injury. In addition, Platelet Distribution Width (PDW) values reflect variations in platelet size and are often associated with inflammatory activity. This study analyzed the histological features and differences in necrotizing leukocyte counts and PDW values in dogs with mild and severe dermatitis. The results showed that necrotizing neutrophils in mild dermatitis (4.9 ± 5.2) were lower than those in severe dermatitis (5.4 ± 3.3), but the results of the independent t-test showed that the difference was not significant (P > 0.05). The opposite situation in eosinophils and basophils, where eosinophils that experienced necrosis in mild dermatitis (2.5 ± 11) were higher than those in severe dermatitis (0 ± 0), while basophils that experienced necrosis were higher in mild dermatitis (9.6 ± 17) than in severe dermatitis (0 ± 0), but the results of the independent t-test showed that the difference was significant (P < 0.05). The PDW value in mild dermatitis (15 ± 2.4) was greater than that in severe dermatitis (14.5 ± 2), but not significantly different (P > 0.05), it can be concluded that the severity of dermatitis does not affect platelet size. Further research needs to be done on health status by looking at other indicators such as the presence of lymphocytes and monocytes in dogs with mild derrmatitis and severe dermatitis.
ISTOPATHOLOGY OF THE BRAIN OF WHITE RATS UPON ADMINISTRATION OF MIMOSIN FROM SIMPLISIA OF LEUCAENA LEAVES Yuyun Setia Ningsih; I Ketut Berata; Ni Luh Eka Setiasih; I Made Kardena; Samsuri; I Made Merdana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p46

Abstract

Lamtoro (Leucaena leucocephala) is a widely used cattle feed in Indonesia due to its high protein, flavonoid, and tannin content. However, it contains mimosine, a compound whose effects on the liver remain poorly understood. This study aimed to investigate the histopathological effects of mimosine on the liver of experimental animals. Twenty male Wistar rats (Rattus norvegicus), aged 2 months (150–200 g), were divided into four groups: a negative control (P0), a positive control given pure mimosine (5 mg/head/day, P1), and two groups administered mimosine from lamtoro leaf simplisia at 50 mg/head/day (P2) and 150 mg/head/day (P3). Treatments were administered orally for 14 days. On day 15, liver samples were collected, fixed in 10% neutral buffered formalin (NBF), and processed for histopathological analysis using hematoxylin-eosin (H&E) staining. Lesions (congestion, inflammation, and necrosis) were scored on a scale of 0 (normal) to 3 (severe). Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results showed that pure mimosine (5 mg) and lamtoro simplisia (50 mg and 150 mg) induced significant hepatic congestion compared to controls, while inflammation was not statistically significant. Necrosis was not observed in any group. No significant difference was found between the two doses of lamtoro simplisia. These findings suggest that mimosine, whether pure or derived from lamtoro leaves, induces mild to moderate liver damage, primarily congestion, but further research is needed to clarify its long-term effects.
Co-Authors I Ketut Berata I Made Kardena I Made Merdana Ni Ketut Suwiti Nyoman Sadra Dharmawan Patricia Amelia Debianti Wagut Samsuri Sri Kayati Widyastuti Yuyun Setia Ningsih