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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pusat Inovasi Masyarakat
Maida, Marciano Oscar
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Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering Social Sciences

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Pendugaan Peptida Bioaktif Cadangan Protein Koro Pedang Hasil Hidrolisis Protease Tubuh Melalui Pendekatan In Silico Maida, Marciano Oscar; Sitanggang, Azis Boing; Budijanto, Slamet
Jurnal Teknologi dan Industri Pangan Vol. 36 No. 2 (2025): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2025.36.2.288

Abstract

The jack bean plant (Canavalia ensiformis L.) contains storage proteins composed of essential amino acids, and the biological activity of these components occurs after a hydrolysis process. The hydrolysis of peptide bonds that appear in the digestive tract can be identified using an in silico approach that utilises a bioinformatics web page. This study aims to predict bioactive peptides resulting from the hydrolysis of storage proteins in the jack beans using a web-based in silico method. Based on literature studies, the jack bean’s dominant storage proteins include canavalin, concanavalin A, and concanavalin B. The amino acid sequences of each type of protein were obtained from the Universal Protein Resource (UniProt) page. Simulations of hydrolysis or cleavage of peptide bonds in the body using Gastrointestinal System (GIS) enzymes, in the form of pepsin, trypsin, and chymotrypsin or a combination thereof, were carried out in the Expert Protein Analysis System (ExPASy) portal on the Peptide Cutter page. The bioactive peptides resulting from the cleavage were identified for their suitability based on the literature using the BIOPEP-UWM database. The simulation results indicate that all jack bean storage proteins possess biological properties, including antihypertensives, antidiabetics, and antioxidant effects. Concanavalin A produced the highest bioactive peptide yield of 82.89%, followed by concanavalin B at 64.23%, and canavalin at 48.95%. The estimation of bioactive peptides from jack bean storage proteins by hydrolysis of body proteases using an in silico approach proved helpful. It can be further applied to other protein sources using different enzyme combinations.
Edukasi Pengelolaan Sampah dan Budidaya Maggot Black Soldier Fly (BSF) di Desa Cihideung Ilir, Kecamatan Ciampea, Bogor Hidayatullah, Raihan Muhammad Iqbal; Faishal, Muhammad Ariq; Graviola, Cantika; Aji, Dhikma Yogi Senasta; Mubarrak, Ramadhita Adji; Sakinah, Linda; Ahadan, Alfan; Finaldin, Muhammad Alhas; Farmayanti, Narni; Maida, Marciano Oscar
Jurnal Pusat Inovasi Masyarakat Vol. 4 No. 2 (2022): Oktober 2022
Publisher : Direktorat Pengembangan Masyarakat Agromaritim, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpim.4.2.40-50

Abstract

Indonesia dikenal sebagai salah satu negara yang menghasilkan sampah dengan jumlah terbanyak di dunia. Kementerian Lingkungan Hidup dan Kehutanan melaporkan pada tahun 2021 terdapat 21,88 juta ton sampah dihasilkan dengan 35,68% diantaranya sampah tidak terkelola. Berdasarkan Sistem Informasi Pengelolaan Sampah Nasional, komposisi sampah yang dihasilkan berupa 41% jenis sisa makanan dan 40,9% bersumber dari rumah tangga. Besarnya jumlah limbah pangan menjadi masalah yang harus diselesaikan. Pengetahuan masyarakat Desa Cihideung Ilir tentang sampah dan pengelolaannya masih minim. Fasilitas pendukung yang kurang pun turut memperburuk kondisi tersebut. Oleh karena itu, diperlukan edukasi dan sosialisasi akan hal tersebut baik kepada orang dewasa maupun anak-anak agar dapat diterapkan sejak dini. Salah satu pengolahannya dapat dilakukan dengan memanfaatkan media budidaya maggot BSF (Black Soldier Fly). Limbah pangan dapat dimanfaatkan sebagai pakan maggot, karena maggot memiliki kemampuan mendegradasi bahan organik. Maggot yang telah berkembang sempurna dapat dimanfaatkan oleh warga sebagai pengembangan ekonomi kreatif bagi generasi muda. Cara pengelolaan sampah organik disampaikan melalui kegiatan penyuluhan, kerja bakti, serta pelatihan langsung kepada warga Desa Cihideung Ilir. Kegiatan tersebut diharapkan dapat membantu menyelesaikan permasalahan sampah organik di lingkungan warga.
Co-Authors Ahadan, Alfan Aji, Dhikma Yogi Senasta Azis Boing Sitanggang Faishal, Muhammad Ariq Finaldin, Muhammad Alhas Graviola, Cantika Hidayatullah, Raihan Muhammad Iqbal Mubarrak, Ramadhita Adji Narni Farmayanti Sakinah, Linda Slamet Budijanto