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All Journal AGRIVITA, Journal of Agricultural Science Journal of Tropical Crop Science
Qazizadah, Ahmad Zubair
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Agriculture, Biological Sciences & Forestry

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Morpho-Physiological Responses of Basil (Ocimum basilicum L.) Microgreens to Chitosan Application and Planting Density Wei, Frederick Tung Chun; Nakasha, Juju Jaafar; Qazizadah, Ahmad Zubair
AGRIVITA Journal of Agricultural Science Vol 48, No 1 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.4806

Abstract

Microgreens are popular culinary ingredients due to their higher nutrient and antioxidant content compared to their mature counterparts. This study was conducted to enhance the seed germination, growth, and yield of basil as a microgreen. The study was divided into two experiments: the first involved basil seeds treated with six chitosan concentrations, including 0, 0.2, 0.4, 0.6, 0.8, and 1% v/v. In the second experiment, the seeds were planted at three densities: 110, 220, and 330 g/m². Based on the results, increasing in concentration of chitosan to an extent led to an increase in seed germination and microgreen growth performances, where 0.6% was the best out of all the treatments as it recorded the highest germination percentage (65%), germination speed (6.918 seeds per day), seed vigour index (4807), seedling height (7.40 cm), root length (3.60 cm), stem diameter (0.56 mm) and leaf area (0.62 cm²). In addition, 220 g/m² was found to be the optimal planting density among the three treatments, due to increased yield and a marketable appearance. Future research is needed to evaluate the nutritional and phytochemical contents.
Improvement of Chlorophyll, Antioxidant Properties, and Biomass Yield in Sweet Basil (Ocimum basilicum L.) Using Chitosan at Various Growing Stages Qazizadah, Ahmad Zubair; Nakasha, Jaafar Juju; Sinniah, Uma Rani; Wahab, Puteri Edaroyati Megat
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.114-133

Abstract

Sweet basil (Ocimum basilicum L.) is known for its numerous health-promising antioxidant phytochemicals and is primarily used in nutritive, medicinal, and cosmetic products. Previous attempts to increase the antioxidant content of sweet basil were associated with disadvantages, including ecological problems, reduced biomass yield, and increased cost. Alternatively, the current study aimed to improve selected antioxidants and biomass yield by using chitosan as an organic and cost-effective growth promoter. In this experiment, a total of four different concentrations of chitosan were applied ( 0%, 0.2%, 0.4%, and 0.6% v/v) at two different growing stages: early growth stage (GS1: 45-day-old plant), late growth stage (GS2: 65-day-old plant), and at both growth stages (known as GS3: 45 & 65-day-old plants). Results showed that plants treated with 0.4% chitosan at GS1 reached the highest chlorophyll a (4.33 mg/cm2), chlorophyll b (2.50 mg/cm2), total chlorophyll (6.84 mg/cm2), total leaf area (2234.31 cm2), total dry biomass (22.72 g per plant), total flavonoid content (33.23 mg QUE/g DE) and DPPH inhibition (92.34%) compared to other treatments. Based on the yield and phytochemical content, it is recommended to apply chitosan at 4% on the 45-day-old plant.
Co-Authors Nakasha, Jaafar Juju Nakasha, Juju Jaafar Uma Rani Sinniah Wahab, Puteri Edaroyati Megat Wei, Frederick Tung Chun