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All Journal Journal of Food and Pharmaceutical Science Journal of Tropical Crop Science
Uma Rani Sinniah
Department of Crop Science, Universiti Putra Malaysia, 43400 UPM Serdang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Changes in Gibberellic Acid (GA3) Content in Oryza sativa Due to Paclobutrazol Treatment Syahputra, Bambang S.A.; Sinniah, Uma Rani; SR, Syed Omar; Ismail, Mohd. Razi
Journal of Food and Pharmaceutical Sciences Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.451 KB) | DOI: 10.14499/jfps

Abstract

The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.Keywords: analysis, gibberellic acid, paclobutrazol, HPLC
Improvement of Chlorophyll, Antioxidant Properties, and Biomass Yield in Sweet Basil (Ocimum basilicum L.) Using Chitosan at Various Growing Stages Qazizadah, Ahmad Zubair; Nakasha, Jaafar Juju; Sinniah, Uma Rani; Wahab, Puteri Edaroyati Megat
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.114-133

Abstract

Sweet basil (Ocimum basilicum L.) is known for its numerous health-promising antioxidant phytochemicals and is primarily used in nutritive, medicinal, and cosmetic products. Previous attempts to increase the antioxidant content of sweet basil were associated with disadvantages, including ecological problems, reduced biomass yield, and increased cost. Alternatively, the current study aimed to improve selected antioxidants and biomass yield by using chitosan as an organic and cost-effective growth promoter. In this experiment, a total of four different concentrations of chitosan were applied ( 0%, 0.2%, 0.4%, and 0.6% v/v) at two different growing stages: early growth stage (GS1: 45-day-old plant), late growth stage (GS2: 65-day-old plant), and at both growth stages (known as GS3: 45 & 65-day-old plants). Results showed that plants treated with 0.4% chitosan at GS1 reached the highest chlorophyll a (4.33 mg/cm2), chlorophyll b (2.50 mg/cm2), total chlorophyll (6.84 mg/cm2), total leaf area (2234.31 cm2), total dry biomass (22.72 g per plant), total flavonoid content (33.23 mg QUE/g DE) and DPPH inhibition (92.34%) compared to other treatments. Based on the yield and phytochemical content, it is recommended to apply chitosan at 4% on the 45-day-old plant.
Co-Authors Bambang Surya Adji Syahputra Mohd. Razi Ismail Nakasha, Jaafar Juju Qazizadah, Ahmad Zubair Syed Omar SR Wahab, Puteri Edaroyati Megat