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This Author published in this journals
All Journal Journal of Food and Pharmaceutical Science AGRIVITA, Journal of Agricultural Science Journal of Tropical Crop Science
Uma Rani Sinniah
Department of Crop Science, Universiti Putra Malaysia, 43400 UPM Serdang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Changes in Gibberellic Acid (GA3) Content in Oryza sativa Due to Paclobutrazol Treatment Syahputra, Bambang S.A.; Sinniah, Uma Rani; SR, Syed Omar; Ismail, Mohd. Razi
Journal of Food and Pharmaceutical Sciences Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.451 KB) | DOI: 10.14499/jfps

Abstract

The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.Keywords: analysis, gibberellic acid, paclobutrazol, HPLC
Improvement of Chlorophyll, Antioxidant Properties, and Biomass Yield in Sweet Basil (Ocimum basilicum L.) Using Chitosan at Various Growing Stages Qazizadah, Ahmad Zubair; Nakasha, Jaafar Juju; Sinniah, Uma Rani; Wahab, Puteri Edaroyati Megat
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.114-133

Abstract

Sweet basil (Ocimum basilicum L.) is known for its numerous health-promising antioxidant phytochemicals and is primarily used in nutritive, medicinal, and cosmetic products. Previous attempts to increase the antioxidant content of sweet basil were associated with disadvantages, including ecological problems, reduced biomass yield, and increased cost. Alternatively, the current study aimed to improve selected antioxidants and biomass yield by using chitosan as an organic and cost-effective growth promoter. In this experiment, a total of four different concentrations of chitosan were applied ( 0%, 0.2%, 0.4%, and 0.6% v/v) at two different growing stages: early growth stage (GS1: 45-day-old plant), late growth stage (GS2: 65-day-old plant), and at both growth stages (known as GS3: 45 & 65-day-old plants). Results showed that plants treated with 0.4% chitosan at GS1 reached the highest chlorophyll a (4.33 mg/cm2), chlorophyll b (2.50 mg/cm2), total chlorophyll (6.84 mg/cm2), total leaf area (2234.31 cm2), total dry biomass (22.72 g per plant), total flavonoid content (33.23 mg QUE/g DE) and DPPH inhibition (92.34%) compared to other treatments. Based on the yield and phytochemical content, it is recommended to apply chitosan at 4% on the 45-day-old plant.
Assessing the Impact of Elevated Temperature and Water Limitation on Yield Production, and Seed Longevity of Aerobic Rice cv. MRIA 1 Amirullah, Nurulnathasa; Abdul Rahman, Siti Maslizah; Aani, Siti Nur Anisah; Sinniah, Uma Rani
AGRIVITA Journal of Agricultural Science Vol 48, No 2 (2026): IN PRESS
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i0.4898

Abstract

This research was conducted at the Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA (UiTM) from February to May 2021. The study objectives were to determine the effects of environmental factors, water limitation (WL) and high temperature (HT), on yield production and seed quality development of aerobic rice cv. MRIA 1. The seed was cultivated in a glass house (25/45°C min/max) and subjected to 3 days of WL and HT (25/40ºC 12 photoperiod) at different seed development stages [8, 14, 21, and 28 days after anthesis (DAA)] with four replications. Plants treated from 21 to 28 DAA showed tolerance to WL and HT, while the detrimental effect occurred at 8 DAA and 14 DAA. Normal germination (>90%) was obtained from the control, and the plant had reached physiological maturity at 21 and 28 DAA. As plant treated to environmental stress, the amylose concentration obtained ranges from 9 to 14%. The lowest seed longevity (p50) obtained from plants treated at 14 DAA recorded only 3 days, and the longest was from control seed obtained for 26 days. In conclusion, exposing cv. MRIA to HT and WL at late maturation stage has less effect on yield production and seed quality development.
Co-Authors Aani, Siti Nur Anisah Abdul Rahman, Siti Maslizah Amirullah, Nurulnathasa Bambang Surya Adji Syahputra Mohd. Razi Ismail Nakasha, Jaafar Juju Qazizadah, Ahmad Zubair Syed Omar SR Wahab, Puteri Edaroyati Megat