This Author published in this journals
All Journal Jurnal Riset Farmasi
P.H, Dian Pramana
Unknown Affiliation

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

Aktivitas Antibakteri Ekstrak Etanol Daun Telang Biru (Clitoria ternatea L.) terhadap Escherichia coli P.H, Dian Pramana; Nur, Amran; Rajih, Muhammad Fakhrur; Fiskia, Ermalyanti
Jurnal Riset Farmasi Volume 5, No. 2, Desember 2025, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v5i2.8530

Abstract

Abstract. Infectious diseases are still the main cause of death in several developing countries, one of which is Indonesia. One of the bacteria that causes infections in humans is Escherichia coli . Butterfly pea flowers (Clitoria ternatea L.) contain various secondary metabolites including alkaloids, flavonoids, saponins, tannins, steroids, and terpenoids. Flavonoids are secondary metabolites that have the potential as antibacterials. This study aims to determine the antibacterial power and optimal concentration of ethanol extract of blue butterfly pea leaves that can inhibit the growth of Escherichia coli  bacteria. The extraction method used was soxhletation with 70% ethanol solvent and antibacterial testing used the disc diffusion method with several variations in the concentration of blue butterfly pea leaf extract, namely 10%, 20%, 30%, 40%, and 50%.. Amoxicillin was used as a positive control while DMSO was used for the negative control. Antibacterial activity is indicated by the diameter of the inhibition zone which is then measured and grouped according to the categories very strong, strong, moderate, and weak. Research data on 70% ethanol extract of blue butterfly pea leaves has antibacterial activity against Escherichia coli  bacteria which is included in the weak category. The optimum concentration of ethanol extract of blue butterfly pea leaves that can inhibit Escherichia coli  bacteria is at a concentration of 50% with an inhibition zone diameter of 3.33 mm. Abstrak. Penyakit infeksi masih menjadi penyebab utama kematian di sejumlah negara berkembang, salah satunya adalah Indonesia. Salah satu bakteri yang menyebabkan infeksi pada manusia adalah Escherichia coli. Bunga telang (Clitoria ternatea) mengandung berbagai metabolit sekunder termasuk alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Flavonoid merupakan metabolit sekunder yang memiliki potensi sebagai antibakteri. Penelitian ini bertujuan mengetahui daya antibakteri serta konsentrasi optimal dari ekstrak etanol daun telang biru yang dapat menghambat pertumbuhan bakteri Escherichia coli.. Metode ekstraksi yang digunakan secara soxhletasi dengan pelaur etanol 70% serta pengujian antibakteri menggunakan metode difusi cakram dengan beberapa variasi konsentrasi ekstrak daun telang biru yaitu 10%, 20%, 30%, 40%, dan 50%. Sebagai kontrol positif digunakan Amoxicillin sedangan untuk kontrol negatif digunakan DMSO. Aktivitas antibakteri ditunjukkan dengan diameter zona hambat kemudian diukur dan dikelompokkan berdasarkan kategori sangat kuat, kuat, sedang, dan lemah. Data penelitian ekstrak etanol 70% daun telang biru memiliki aktivitas antibakteri terhadap bakteri Escherichia coli yang termasuk dalam kategori lemah. Konsentrasi optimum ekstrak etanol daun telang biru yang mampu menghambat bakteri Escherichia coli yaitu pada konsentrasi 50% dengan diameter zona hambat sebesar 3,33 mm.