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All Journal Techno Agriculturae Studium of Research
Ali, Fatima
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Agriculture, Biological Sciences & Forestry

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THE USE OF RECOMBINANT DNA TECHNOLOGY TO ENHANCE BETA-CAROTENE CONTENT IN CASSAVA (GOLDEN CASSAVA) Abakar, Sonia; Ali, Fatima; Saleh, Mahamat
Techno Agriculturae Studium of Research Vol. 2 No. 6 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/agriculturae.v2i6.2965

Abstract

Cassava (Manihot esculenta) is a staple crop widely grown in tropical regions, providing a major source of carbohydrates. However, its nutritional content is limited, particularly in essential micronutrients such as provitamin A. Beta-carotene, a precursor of vitamin A, plays a critical role in human health, particularly in preventing vitamin A deficiency, which is prevalent in many developing countries. Enhancing beta-carotene content in cassava could significantly improve its nutritional value and address public health concerns related to micronutrient malnutrition. The objective of this study is to use recombinant DNA technology to genetically engineer cassava varieties with enhanced beta-carotene content, creating what is commonly referred to as “Golden Cassava.”This research employed genetic transformation techniques, specifically Agrobacterium-mediated transformation, to introduce genes responsible for the biosynthesis of beta-carotene into cassava. Candidate genes, including those from the daffodil and maize, were selected to enhance the carotenoid biosynthesis pathway. Transgenic cassava plants were developed, and molecular analysis, including PCR and Southern blotting, was used to confirm the presence and integration of the introduced genes. Beta-carotene content in the transgenic plants was measured using high-performance liquid chromatography (HPLC). The results showed that the genetically modified cassava plants exhibited a significant increase in beta-carotene content compared to the wild-type varieties. The transgenic lines demonstrated enhanced nutritional quality without affecting other agronomic traits. In conclusion, recombinant DNA technology has proven to be an effective tool for biofortifying cassava with beta-carotene. This approach offers a promising strategy for addressing vitamin A deficiency and improving the nutritional value of cassava in regions where it is a major food source.
AGROECOLOGY IN ACTION: DESIGNING FARMING SYSTEMS THAT BALANCE ECOLOGICAL INTEGRITY AND AGRICULTURAL PRODUCTIVITY Ali, Fatima; Saleh, Mahamat; Assingar, Nadia
Techno Agriculturae Studium of Research Vol. 3 No. 1 (2026)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/agriculturae.v3i1.3612

Abstract

Agroecology has emerged as a transformative approach to address the growing challenges of environmental degradation, climate variability, and declining sustainability in conventional agricultural systems. Intensive farming practices have often prioritized short-term productivity at the expense of ecological integrity, leading to soil degradation, biodiversity loss, and reduced system resilience. This study aims to analyze how agroecological principles can be operationalized in the design of farming systems that simultaneously maintain ecological integrity and enhance agricultural productivity. The research employed a mixed-methods approach combining field-based comparative analysis of agroecological and conventional farming systems, ecological assessments, and secondary data review. Key indicators included crop yield, soil health parameters, biodiversity indices, input-use efficiency, and ecosystem service provision. The results indicate that agroecological farming systems achieved comparable or higher productivity levels than conventional systems while significantly improving soil quality, biodiversity, and resource-use efficiency. Enhanced ecological interactions, such as nutrient cycling and biological pest regulation, contributed to system stability and reduced dependency on external inputs. The study concludes that agroecology provides a viable framework for designing resilient farming systems capable of balancing productivity with ecological sustainability. Adoption of agroecological practices represents a strategic pathway toward sustainable food systems, ecosystem conservation, and long-term agricultural resilience.
Co-Authors Abakar, Sonia Assingar, Nadia Saleh, Mahamat