Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal JBIO: jurnal biosains (the journal of biosciences)
Thebez, Vanessa Angela
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Efectiveness Of Carica papaya Seeds Ethanol Extract With Metronidazole Against Porphyromonas gingivalis In Antibacterial And Antibiofilm Activity Thebez, Vanessa Angela; Soesanto, Sheila; Natassya, Priska
JURNAL BIOSAINS Vol. 11 No. 3 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i3.68420

Abstract

Introduction: Porphyromonas gingivalis promotes the growth of periodontal biofilm. Metronidazole is one of the adjunctive therapies for periodontitis. The combination of metronidazole with natural products such as Carica papaya seeds ethanolic extract, which has proven to have antibacterial effects is expected to reduce the side effects caused by metronidazole. Objective: To determine the antibacterial and antibiofilm effects of C. papaya seeds ethanolic extract combined with metronidazole against P. gingivalis. Methods: In vitro laboratory experiment using post-test only control group design. The sample used were 100% C. papaya seeds ethanolic extract, combination of 1000 µg/mL metronidazole with extract 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%, 1000 µg/mL and 2000 µg/mL metronidazole as positive control, and distilled water as negative control. Antibacterial test was performed using plate count method and antibiofilm using microtiter plate biofilm assay method. Results: Carica papaya seeds ethanolic extract alone showed effective antibacterial and antibiofilm effects against P. gingivalis. The combination of 1000 µg/mL metronidazole with extract 6.25% to 100% showed an antibacterial effect equivalent to 2000 µg/mL metronidazole. The combination of 1000 µg/mL metronidazole with 100% extract showed antibiofilm effects in 1, 3, and 24 hours time incubation. The combination of extract and 1000 µg/mL metronidazole demonstrated the best antibiofilm effect during the biofilm maturation phase. Conclusion: The combination of 1000 µg/mL metronidazole with ethanol extract of C. papaya seeds exhibited antibacterial and antibiofilm effects against P. gingivalis.
Co-Authors Natassya, Priska Soesanto, Sheila