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Yusfi Afidah
Universitas Jember
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Metode Direct PCR yang Cepat dan Hemat Biaya untuk DNA Barcoding Anggrek Obat Dendrobium crumenatum Sw. Yusfi Afidah; Mukhamad Su'udi
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v7i2.12893

Abstract

This study aims to develop a rapid and cost-effective Direct PCR method for amplifying the rbcL gene without requiring DNA isolation, particularly for Dendrobium crumenatum Sw., an orchid species known for its traditional medicinal uses. The method involved soaking fresh leaves of D. crumenatum in TE buffer, followed by incubation at 55ºC. The incubation product was Directly used as a PCR template. The results showed a clear DNA band of 600 bp, corresponding to the target rbcL gene. BLAST analysis confirmed that the DNA sequence shared 84-85% identity with other species in GenBank. In conclusion, the Direct PCR method proved to be effective for DNA barcoding applications, offering advantages in speed and cost-efficiency over conventional DNA isolation methods. Keywords: Medicinal Orchids, Dendrobium crumenatum Sw., Direct PCR, DNA Barcoding
Co-Authors Mukhamad Su'udi