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All Journal Grouper: Jurnal Ilmiah Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Mohammad Fadjar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Veterinary

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Effect of Extracellular Crude Protein (ECP) on expression IL-1, IL-6, IFN- in nile tilapia (Oreochromis niloticus) Cucun Herlina; Mohammad Fadjar; Maftuch Maftuch; Uun Yanuhar
Depik Vol 14, No 3 (2025): SEPTEMBER 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.3.43338

Abstract

Modern strategies incorporate preventive measures such as extracellular products, as soluble proteins with antigenic properties, as well as generating a broad immune response. The aim of research is to determine and analyze the effect of the extracellular product of E. tarda on enhancing the immunity of Nile tilapia (O. niloticus). The research design with treatment durations of d0, 3rd, 6th, 9th day, and control (A, B, C, D, K). ECP vaccination was injected intraperitoneally at a dose of 0.1 ml/fish. The parameters analyzed are the expression of IL-1, IL-6, and IFN-, phagocytic activity, leukocyte differential, total white blood cells, and relative percent survival. The results of phagocytosis activity were highest in treatment C at 84.18% 1.81. The highest leukocyte differential in treatment C was lymphocytes at 56% 2.00, monocytes at 28% 2.00, and 16% 0.00, with a total leukocyte count of 29 x 10^3 cells 2.64, and RPS values ranging from 83.33% to 100% over 9 days of post-vaccination maintenance. Then, post-vaccination, there was an increase in the expression levels of the cytokine genes IL-1 and IL-6, but there was no increase in the expression levels of the IFN- gene compared to the control in Nile tilapia. (O. niloticus).Keywords:ECPIL-1IL-6IFN-Immune Response
Effects of Feed Type and Culture System on Growth and Survival of Panulirus homarus Puerulus in Situbondo, East Java Hendra, Hendra; Abdul Rahem Faqih; Mohammad Fadjar; Lailatus Silfiyah
Grouper Vol. 17 No. 1 (2026): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v17i1.397

Abstract

Lobster (Panulirus homarus) aquaculture in Indonesia remains limited due to suboptimal feed management and culture systems, resulting in low growth and survival rates. This study aimed to evaluate the effects of different feed types and culture systems on growth performance, survival rate, feed efficiency, amino acid content, and carbonic anhydrase enzyme activity in lobster puerulus. The study was conducted using a factorial randomized block design with two factors: culture media (bottom cage, floating cage, and fiber tank) and feed types (kijing clams, trash fish, and shrimp head waste). Each treatment was replicated three times at a stocking density of 30 individuals per square meter. The results showed that the highest survival rate (90%) and best feed conversion ratio (6.6) were obtained in fiber tanks with kijing clam feed. The highest growth performance (5.11 g) and enzyme activity were recorded in fiber tanks with shrimp head waste feed. Amino acid content, particularly leucine, was also highest under the same treatment. Statistical analysis indicated that both feed type and culture system significantly influenced lobster performance, with strong relationships observed between feed composition and physiological responses. The conclusion is that the combination of appropriate feed and a controlled culture system is essential for improving lobster aquaculture performance. Fiber-based systems with high-quality feed provide optimal conditions for growth, survival, and metabolic activity, supporting more efficient and sustainable lobster farming.
Co-Authors Abdul Rahem Faqih Cucun Herlina Hendra Hendra Lailatus Silfiyah Maftuch Maftuch Yanuhar, Uun