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All Journal Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran International Journal of Health Science
Fredericus Pramonodjati
Universitas Duta Bangsa Surakarta
Dentistry Health Professions Public Health Social Sciences

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Activity of Enzyme in Escherichia Coli Through Molecular Techniques Liss Dyah Dewi Arini; Fredericus Pramonodjati
International Journal Of Health Science Vol. 5 No. 2 (2025): International Journal of Health
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/ijhs.v5i2.4991

Abstract

Enzymes are protein biomolecules that act as catalysts in various biochemical reactions, both in biological systems and industrial applications. The uniqueness of enzymes lies in their ability to accelerate the rate of reactions with a high degree of specificity towards certain substrates without undergoing permanent changes in their chemical structure. Enzyme activity is strongly influenced by various environmental factors, such as temperature, pH, substrate concentration, and the presence of inhibitors or activators. Therefore, quantitative testing of enzyme activity is an important step in understanding the characteristics of enzymes and their applications in various fields. Escherichia coli produces Extended-Spectrum Β-Lactamase Enzyme (ESBL) and plays a role in damaging the structure of beta lactam antibiotics so that the antibiotics cannot kill bacteria. Bacteria that produce ESBLs need to be watched out for because ESBLs are produced by genes located on plasmids, which can easily be transferred to other bacteria, and often also carry resistance genes to other antibiotics. Objective: to accurately measure the activity or concentration of enzymes in samples of Escherichia coli bacteria and understand the influence of variables such as substrate concentration on the reaction rate. Method: spectrophotometry through enzyme extraction, making a standard curve and testing enzyme activity against variations in substrate concentration. Results: samples with concentrations of 0.1 and 0.3 showed good and appropriate absorbance results. However, in the sample 0.5 ; 0.7 ; and 1.0 indicates an absorbance number that is slightly higher than it should be. Conclusion: the enzyme in Escherichia coli bacteria has good activity at sample concentrations of 0.1 and 0.3.
Implikasi Patogen Bawaan Makanan yang Resisten terhadap Antibiotik bagi Keamanan Makanan Jalanan di Surakarta, Jawa Tengah, Indonesia Liss Dyah Dewi Arini; Fredericus Pramonodjati; Annisa Rahmawati; Sovia Meldalina; Putri Aulia Ramadhani
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 4 No. 3 (2026): Juli : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v4i3.6719

Abstract

The safety of street-vended food is a critical public health issue, as such foods can serve as vehicles for the transmission of pathogenic bacteria that cause foodborne illnesses. This threat is exacerbated by the emergence of antibiotic-resistant bacteria, which complicates the treatment of human infections. This study aimed to assess the impact of drug-resistant pathogenic bacteria on the safety of street food in Surakarta City, Central Java Province, Indonesia. A descriptive laboratory-based method was employed to analyze 20 street food samples collected from various locations in Surakarta. Isolated bacteria were tested for susceptibility using the disk diffusion method (Kirby–Bauer) against four antibiotics: chloramphenicol, levofloxacin, amikacin, and ampicillin. The results revealed varying inhibition zone sizes across the antibiotics, indicating differing levels of bacterial sensitivity. On average, chloramphenicol demonstrated the highest antibacterial activity, followed by ampicillin, amikacin, and levofloxacin. Conversely, certain isolates from specific food samples showed no inhibition zones against any of the tested antibiotics, suggesting the presence of multidrug-resistant (MDR) bacteria. These findings indicate that street food can act as a source for the spread of antibiotic-resistant bacteria, posing potential health risks to the public. Therefore, improvements in food hygiene and sanitation, routine food quality monitoring, and surveillance of antibiotic resistance in read to-sell foods are essential aligned with the "One Health" approach—to mitigate the spread of antimicrobial resistance. The study's results are expected to serve as a scientific basis for refining street food safety management policies and preventing the rise of antibiotic resistance in Indonesia.
Co-Authors Annisa Rahmawati Putri Aulia Ramadhani Sovia Meldalina