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All Journal Jurnal Medik Veteriner Asian Journal of Agriculture
NHAN, PHAN
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Dietary lotus seed powder enhances semen quality and storage stability in Mia roosters NHAN, PHAN; TRI, NGUYEN MINH
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090226

Abstract

Abstract. Nhan P, Tri NM. 2025. Dietary lotus seed powder enhances semen quality and storage stability in Mia roosters. Asian J Agric 9: 598-606. Semen quality, a key determinant of reproductive efficiency in poultry, plays a crucial role in breeding programs and in the conservation of indigenous genetic resources. Natural dietary supplements with antioxidant activity may improve sperm function and preservation. Lotus seed powder (Nelumbinis semen) is rich in bioactive compounds with antioxidant and membrane-stabilizing properties, but its effects on avian reproduction remain unexplored. This study represents the first report in avian species evaluating the impact of dietary lotus seed powder supplementation on semen quality and storage stability in Mia roosters, a Vietnamese indigenous breed. Thirty healthy roosters (10-11 months) were fed either a control diet or diets supplemented with 2% or 4% lotus seed powder for 60 days. Semen was collected every three days and assessed for volume, concentration, motility parameters, morphology, viability, membrane integrity, and chilled storage stability (0-24 h at 4°C). Compared with the control (C), T2 showed higher semen volume (0.48±0.05 mL vs 0.42±0.05 mL), sperm concentration (3.47±0.27×109/mL vs 3.21±0.26×109/mL), VSL (46.25±3.15 µm/s vs 42.35±3.12 µm/s), and viability (91.03±2.68% vs 87.46±2.85%). Abnormal morphology decreased from 9.84±1.21% in C to 8.15±1.12% in T2. Motility after 24 h storage remained higher in T2 (63.20±4.15%) compared with C (51.81±4.91%). Dietary lotus seed powder, particularly at 4% inclusion, significantly improves semen quality by enhancing sperm motility, viability, and membrane integrity, while also prolonging chilled storage stability in Mia roosters. These outcomes underscore its potential as a natural reproductive enhancer with applications in Artificial Insemination (AI) and the conservation of indigenous poultry genetic resources.
Antimicrobial resistance profiles of Staphylococcus aureus isolated from dogs and cats in veterinary clinics in Vinh Long Province, Vietnam Vinh, Truong Phuc; Tri, Nguyen Minh; Nhan, Phan
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.161-171

Abstract

Staphylococcus aureus is a common cause of dermatological, wound, and reproductive tract infections in companion animals, and its increasing antimicrobial resistance poses challenges for effective treatment. Data on resistance patterns in small animals in the Mekong Delta remain limited. This study aimed to determine the prevalence of S. aureus in clinical samples from dogs and cats and to characterize the antimicrobial susceptibility patterns of the isolates. Clinical samples were collected from dogs and cats showing signs of infection at veterinary clinics in Vinh Long Province, Vietnam. S. aureus was isolated using Mannitol Salt Agar and identified by standard biochemical tests. Antimicrobial susceptibility was determined using the Kirby–Bauer disk diffusion method according to CLSI guidelines. Of the 32 clinical specimens examined, 62.5% were positive for S. aureus. The prevalence was higher in dogs than in cats and higher in females than in males. Skin exudates accounted for the largest proportion of positive samples. Among the 60 isolates tested, resistance to ampicillin was highest at 90%. Increased resistance was also observed for erythromycin and trimethoprim–sulfamethoxazole. Susceptibility remained moderate to high for chloramphenicol, tetracycline, and levofloxacin. Multidrug resistance was common, with 22% of isolates resistant to ≥6 antibiotics. S. aureus is a frequent pathogen in small-animal clinical cases in Vinh Long Province and shows considerable resistance to commonly used antibiotics. The high rate of multidrug resistance highlights the need for routine culture and susceptibility testing and supports the implementation of antimicrobial stewardship in veterinary practice.
Co-Authors Tri, Nguyen Minh Vinh, Truong Phuc