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PENGARUH VARIASI PUPUK TERHADAP LAJU PERTUMBUHAN MIKROALGA DIATOM Chaetoceros calcitrans Azkiya, Azmi Binta; Indriyawati, Novi; Wulandari, Destin Retno; Amanda, Vevilia Tri; Supriyanto, Supriyanto
JURNAL AKUAKULTUR, TEKNOLOGI DAN MANAJEMEN PERIKANAN TANGKAP, ILMU KELAUTAN Vol 8 No 1 (2025): JOURNAL OF INDONESIAN TROPICAL FISHERIES
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Muslim Indonesia Makassar, Sulawesi Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/joint-fish.v8i1.598

Abstract

The use of Pro Analis fertilizer with uneconomical prices in microalgae culture has been a problem that has hampered the utilization of microalgae. The use of agricultural fertilizers as a more economical source of nutrients. This study was conducted to determine the density value of microalgae cultured using agricultural fertilizers to determine which agricultural fertilizers support the cultivation of Chaetoceros calcitrans. This study was conducted using an experimental method, the study was conducted on a laboratory scale with 4 treatments with a research design P1 using variations of Urea Silica fertilizer; P2 NPK and silica; and P3 Urea, NPK and Silica with a ratio of Urea; NPK; Silica fertilizer (0.1; 0.1; 0.015 gr / L). The results of data analysis show that the provision of fertilizer variation treatment has a significant difference (≤0.05) and has a positive correlation with a very strong correlation (r = 0.656) and R2 = 0.431 which shows that the provision of fertilizer variation affects the density value of the Chaetoceros calcitrans microalgae by 43.1% with the provision of NPK and silica fertilizer variation treatment (P2) resulting in the highest density of 291 x104 and the largest cell size with a size of 3.566 µm with these results indicating the ability of agricultural fertilizers as a substitute source of nutrients in microalgae culture.
Pengaruh Variasi Media Kultur Terhadap Komposisi Nutrisi Mikroalga Thalassiosira sp. Amanda, Vevilia Tri; Indriyawati, Novi; Azkiya, Azmi Binta; Wulandari, Destin Retno; Bachri, Abdul Rozak; Winata, Dimas Candra
Jurnal Kelautan Vol 19, No 1: April 2026
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v19i1.33676

Abstract

ABSTRAKMikroalga Thalassiosira sp. merupakan sumber bioaktif yang potensial karena kandungan protein, lipid, dan karbohidratnya tinggi. Penelitian ini bertujuan untuk mengevaluasi pengaruh perbedaan media kultur untuk peningkatan biomassa dan nutrisi. Rancangan Acak Kelompok (RAK) dengan tiga perlakuan dan tiga ulangan digunakan, dengan analisis proksimat meliputi kadar air, lemak, abu, protein, dan karbohidrat. Data dianalisis menggunakan One-Way ANOVA dan dilanjutkan dengan Uji Tukey HSD untuk mengetahui perbedaan antar perlakuan. Hasil analisis menunjukkan perbedaan signifikan antar media (p 0,05). Kandungan protein tertinggi terdapat pada media air laut steril (PA) sebesar 30,42%, lemak tertinggi pada media air laut tidak steril (PC) sebesar 24,33%, dan abu tertinggi pada media air buatan (PB) sebesar 2,44%. Temuan ini mengindikasikan bahwa media air laut steril (PA)memberikan kecenderungan paling efektif dalam peningkatan  kandungan nutrisi, khususnya protein, pada Thalassiosira sp. tanpa mengabaikan peran media lain dalam mempengaruhi kandungan nutrisi yang berbeda. Oleh karena itu, pemilihan media kultur perlu disesuaikan dengan tujuan produksi biomassa atau komponen  nutrisi yang diinginkan.Kata Kunci: Thalassiosira sp., nutrisi, media kulturABSTRACTThalassiosira sp. microalgae are a potential source of bioactive compounds due to their high protein, lipid, and carbohydrate content. This study aimed to evaluate the effect of different culture media on biomass production and nutrient composition. A Randomized Block Design (RBD) with three treatments and three replications was applied. Proximate analysis included moisture, lipid, ash, protein, and carbohydrate contents. Data were analyzed using One-Way ANOVA followed by Tukey’s HSD test to determine significant differences among treatments. The results showed significant differences among culture media (p 0.05). The highest protein content was found in sterile seawater medium (PA) at 30.42%, the highest lipid content in non-sterile seawater medium (PC) at 24.33%, and the highest ash content in artificial seawater medium (PB) at 2.44%. These findings indicate that sterile seawater medium (PA) tends to be the most effective in enhancing nutrient content, particularly protein, in Thalassiosira sp., while other media contribute differently to specific nutrient components. Therefore, the selection of culture media should be adjusted according to the intended biomass production or targeted nutritional components.Keywords: Thalassiosira sp., nutrition, culture media