Utami, Putra Rahmadea
Department Of Medical Laboratory Technology, Faculty Of Health Science, Universitas Perintis Indonesia, Padang, Indonesia

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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

COMBINATION TEST OF CHINESE LEAF EXTRACT (Leucaena leucocephala folium) AND ALOE VERA INHIBITING GROWTH Escherichia coli Putra Rahmadea Utami; Chairani; Hendra Yudha
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1577

Abstract

Chinese petai (Leucaena leucocephala folium) and aloe vera (Aloe vera L.) have medicinal properties among the plants.  The objective of this study was to determine the number of inhibitory zones produced by the ethanol extract of Chinese petai and aloe vera on the growth of Escherichia coli. The research method was In-vitro Experimental Laboratory research design with the Kirby Bauer method. The samples used was Chinese petai and aloe vera with pure strains of E. coli. One Way ANOVA was used to compare the differences in inhibition of Chinese petai and aloe vera on the growth of E. coli. The results of a combination of Chinese petai and aloe vera extract test showed that there were significant differences in the concentration of 25 g, 50 g, 75 g, and 100 g (p <0.05). The ethanol extract of Chinese petai and aloe vera can inhibit the growth of E. coli. From the results of this study found that there was an interaction on the combination of ethanol extract of Chinese petai and aloe vera inhibiting the growth of E. coli with the most effective concentration being 100 g/mL. This study can find out the benefits of petai cina and aloe vera also the public will know the benefits and efficacy of Chinese petai and aloe vera leaves in medicine.
Ability of Ethanol Extract from Ajwa and Sukkari Dates (Phoenix dactylifera L.) in Inhibiting the Growth of Methicillin-Resistant Staphylococcus aureus (MRSA) Putra Rahmadea Utami; Sri Indrayati; Nur Hayatang
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1848

Abstract

Staphylococcus aureus is a pathogenic bacterium that spread throughout the world and still a problem that continues to increase both in hospitals and the community. Infections due to S. aureus usually treated with antibiotics, but in some cases, several strains of S. aureus found to be resistant to antibiotics, such as Methicillin-Resistant Staphylococcus aureus (MRSA). Based on the previous research, the ethanol extract from Ajwa and Sukkari dates formed an inhibitory zone against the MRSA bacteria growth. This study aims to determine the inhibition of the ethanol extract from Ajwa and Sukkari variety of dates (Phoenix dactylifera L.) on the S. aureus growth. The ethanol extract from Ajwa and Sukkari dates with a concentration of 5 mg/mL, 10 mg/mL, 15 mg/mL, and 20 mg/mL resulted in the same inhibition zone with a diameter of ≤ 6 mm which categorized as weak (resistant), whereas the positive control ciprofloxacin had a resistance zone with a diameter of 9 mm. This study results concluded that the ethanol extract of Ajwa and Sukkari dates only has a maximum concentration of 20 mg/mL, which is still classified as a low concentration and has not been able to inhibit MRSA bacteria growth.