Article Per Year (5 Year)
p-Index From 2021 - 2026
0.408
P-Index
This Author published in this journals
All Journal DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Dewinta Nur Alvionita
Teknologi Laboratorium Medik/Fakultas Teknologi Kesehatan/Universitas Megarezky, Makassar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Isolation and Characterization of Cellulolytic Bacteria from Soil around Chicken Coop Latusi Anggriani; Dewinta Nur Alvionita; Saldi Yulistian; Angger Hidayat
DIVERSITAS HAYATI Vol. 1 No. 1 (2023): DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Publisher : UIN Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/11.23-34

Abstract

Bacteria that utilize cellulose as a substrate and convert it into simpler compounds with the help of cellulase enzymes are known as cellulolytic bacteria. Cellulase enzymes are capable of breaking down cellulose into glucose. Cellulolytic bacteria are bacteria that can produce cellulase enzymes and are typically found in areas containing compost, soil rich in leaf litter, as well as agricultural and livestock soils. The aim of this study is to obtain bacterial isolates from soil around a chicken coop. Bacterial isolates from the soil around the chicken coop were cultured on selective CMC media. The successfully isolated bacteria were then purified to obtain single colonies. The obtained single colonies were characterized to determine the type of cellulolytic bacteria that were successfully isolated. Subsequently, testing was conducted to measure the cellulase enzyme activity produced by the obtained isolates. Four isolates of bacteria successfully grew on the selective CMC media. These four isolates were identified as gram-negative bacteria with a rod-shaped cell morphology. Enzyme activity testing was only performed on isolate LD 2, as it exhibited a clear zone indicating enzyme activity. The highest cellulase enzyme activity value was 0.0062 U/mL, with a 30-minute incubation time of the cell supernatant at 37°C, and a specific enzyme activity of 0.1891 U/mg. Based on the conducted research, only one isolate from the soil around the chicken coop exhibited enzyme activity
Isolation of Cellulolytic Bacteria from the Soil Surrounding a Final Landfill Site Latusi Anggriani; Fily Larasari; Dewinta Nur Alvionita
DIVERSITAS HAYATI Vol. 1 No. 2 (2023): DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Publisher : UIN Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/12.18-27

Abstract

Cellulolytic bacteria are capable of producing cellulase enzymes, enabling them to break down cellulose into simpler compounds using cellulose as a growth substrate. These bacteria are commonly found in various environments containing cellulose, such as landfill sites where abundant organic materials are present. This research employed the cup-plate isolation method with the aim of obtaining cellulolytic bacterial isolates from the soil surrounding a final landfill site. Successful isolation of cellulolytic bacteria was achieved from soil samples taken in proximity to the disposal site and cultured using selective CMC media. Two purified isolates were obtained, and their cellulase enzyme activities were examined. Isolate LA2 exhibited higher cellulase enzyme activity than isolate LA1, forming a clear zone index of 0.7778. Further quantitative analysis of isolate LA2 revealed its highest enzyme activity at the fifth minute, measuring 0.098 u/ml with a specific activity of 0.29 u/mg protein. Based on physiological characteristics, Gram staining, and spore staining, isolate bacteria LA2 were identified as Pseudomonas sp.
Co-Authors Angger Hidayat Fily Larasari Latusi Anggriani Latusi Anggriani Saldi Yulistian