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All Journal Jurnal Riset Ilmu Kesehatan Umum dan Farmasi DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Hazli Nurdin
Universitas Perintis Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Public Health

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DETERMINATION OF TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT MATOA LEAVES (Pometia pinnata) BY SOXHLET EXTRACTION Tisa Mandala Sari; Hazli Nurdin; Febti Nengsih; Miftahur Rahmi
DIVERSITAS HAYATI Vol. 3 No. 1 (2025): DIVERSITAS HAYATI: Journal of Conservation and Biological Resources
Publisher : UIN Sulthan Thaha Saifuddin Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/31.12-16

Abstract

The matoa plant (Pometia pinnata) is one of the medicinal plants frequently used for traditional medicine by the Indonesian community. This research aims to determine the total phenolic content and antioxidant activity of matoa leaf extract obtained through the soxhlet extraction method using 70% ethanol as a solvent. The total phenolic content of the matoa leaf ethanol extract was analyzed using the Folin-Ciocalteu method, and the antioxidant activity was tested using the DPPH method with gallic acid as a comparison compound, measured using a UV-Vis Spectrophotometer. The total phenolic content of the ethanol extract from the soxhlet-extracted matoa leaves at the maximum absorption wavelength of 762 nm was found to be 13.98% b/b. The antioxidant activity test of gallic acid yielded an IC50 of 7.922 µg/mL, and the antioxidant activity of the ethanol extract from the soxhlet-extracted matoa leaves at the maximum absorption wavelength of 521 nm yielded an IC50 of 112.854 µg/mL. Based on the IC50 values, it can be concluded that the antioxidant activity of the ethanol extract from the soxhlet-extracted matoa leaves falls into the moderate category, specifically in the range of 100-150 µg/mL. The equivalence of the antioxidant activity of the matoa leaf ethanol extract to gallic acid is 1:14.2456 mg, meaning that 1 mg of gallic acid is equivalent to 14.2456 mg of matoa leaf ethanol extract.
Penetapan Kadar Beta Karoten pada Daun Sirih Hijau (Piper Betle L.) Berdasarkan Ketinggian Tempat Tumbuh Dedi Nofiandi; Hazli Nurdin; Sulha Ananda Putri
Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF) Vol. 4 No. 1 (2026): Januari : Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF)
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/jrikuf.v4i1.953

Abstract

Research has been conducted on determining beta carotene levels in green betel leaves (Piper betle L.) based on the altitude at which they grow. The purpose of this study was to determine the levels and differences in beta carotene levels in green betel leaves based on the altitude where it grows. This research consists of qualitative analysis and quantitative analysis. Qualitative analysis with thin layer chromatography and phytochemical screening tests on the extract, while quantitative analysis with UV-Visible spectrophotometry. The results of qualitative analysis with thin layer chromatography showed that green betel leaf extract contains beta carotene compounds obtained Rf value 0.48 and pure beta carotene Rf value 0.48. Other qualitative analysis with phytochemical screening tests on the three sample acetone extracts originating from the Alahan Panjang, Solok and Pasir Jambak areas contained terpenoid, phenolic, flavonoid compounds and did not contain saponin and alkaloid compounds. The results of quantitative analysis obtained the maximum absorption wavelength of beta carotene is 481 nm with the average beta carotene content in green betel leaves of Alahan Panjang, Paninggahan, Solok Regency, and Pasir Jambak respectively, namely 113.845 mg/100 g, 129.98 mg/100 g, and 7.530 mg/100 g. Data analysis using the One Way Anova test (SPSS 31.0) showed a significant effect of the height of the growing area on the beta carotene content of green betel leaves (P < 0.05).
Co-Authors Dedi Nofiandi Febti Nengsih Miftahur Rahmi Sulha Ananda Putri Tisa Mandala Sari