Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Jurnal Perikanan Universitas Gadjah Mada
Sativa, Putriku Nadzira
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Evaluation of Genetic Variation in Hybrid Catfish (Sangkuriang × Thai) Employing PCR-RAPD Markers Rahmi, Kurnia Anggraini; Sativa, Putriku Nadzira; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri
Jurnal Perikanan UGM (Journal of Fisheries Sciences) Vol 28, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.113208

Abstract

Hybridization is the process of crossing individuals with different genotypes to produce offspring with superior traits. In this study, Sangkuriang catfish were crossed with introduced Thai catfish to obtain heterosis in hybrid seeds. The objective was to analyze the genetic diversity of these hybrid seeds using the PCR-RAPD method. Specifically, the primers OPA-13, OPB-08, OPC-19, and OPD-02 were used. Crossbreeding involved Sangkuriang female and Thai male broodstock, with four pairs of broodfish used via a semi-natural spawning method. After spawning, hybrid seeds from each pair were reared separately in containers under uniform conditions for 1.5 months. DNA samples were then collected from the fin tissue of each replicate of the crosses, and were grouped according to size categories: large (7-8 cm), medium (5-6 cm), small (3-4 cm), and broodstock, with 30 samples per size category for each replicate. The results revealed a polymorphism percentage of 72.95-93.88%, heterozygosity values of 0.167-0.232, and an average genetic distance among hybrid seeds from the three size categories and broodstock ranging from 0.001-0.018.
Co-Authors Dinar Tri Soelistyowati Rahmi, Kurnia Anggraini