Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal G-Tech : Jurnal Teknologi Terapan
Destyano, Azel
Unknown Affiliation
Computer Science & IT Decision Sciences, Operations Research & Management Energy Engineering

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
The Effect of Substrate Concentration and Bacterial Loading on Biobutanol Production from Molasses Using Clostridium acetobutylicum Putri , Zahra Dzaki Roofia; Destyano, Azel; Sari, Ni Ketut; Triana, Nurul Widji; Santi, Sintha Soraya
G-Tech: Jurnal Teknologi Terapan Vol 10 No 2 (2026): G-Tech, Vol. 10 No. 2 April 2026
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v10i2.9288

Abstract

Biobutanol is a promising alternative fuel due to its high energy content and compatibility with existing fuel infrastructure. This study evaluates the effects of molasses substrate concentration and Clostridium acetobutylicum loading on biobutanol production via the Acetone–Butanol–Ethanol (ABE) fermentation process. Molasses concentration was varied at 3%, 4%, 5%, 6%, and 7%, while bacterial loading ranged at 1%, 2%, 3%, 4%, and 5%. Fermentation was performed under anaerobic conditions at 37 °C for 4 days with CaCO₃ as a buffering agent. Unlike previous studies that investigated these parameters individually, this work examines their combined influence using a statistical optimization approach. The highest experimental biobutanol concentration of 6.06% was obtained at a substrate concentration of 6% and a bacterial loading of 5%. Optimization using Response Surface Methodology (RSM) produced a statistically significant quadratic model (p < 0.0001) with good predictive performance (R² = 0.9045; predicted R² = 0.8153). The optimal conditions were predicted at a substrate concentration of 5.220% and a bacterial loading of 4.580%, yielding a biobutanol concentration of 6.087%. These results highlight the importance of simultaneous parameter optimization for improving molasses-based biobutanol production.
Co-Authors Ni Ketut Sari Putri , Zahra Dzaki Roofia Sintha Soraya Santi Trianna, Nurul Widji