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All Journal Bioscientist : Jurnal Ilmiah Biologi
Isnikarita, Rina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Analysis of the Abundance and Morphological Characteristics of Airborne Microorganisms in an Environmental Engineering Laboratory Prawitasari, Diah Ayu; Isnikarita, Rina; Lathifa, Annisa Nur; Rahmawati, Suphia
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.17342

Abstract

This study aimed to analyze the abundance and morphology of airborne microorganisms in the Environmental Engineering Laboratory and to relate their distribution to differences in room conditions and sampling time. Sampling was conducted in two rooms, Room A and Room B, during the morning and afternoon using a microbiological air sampler with Nutrient Agar (NA), Potato Dextrose Agar (PDA), and Plate Count Agar (PCA) media. Statistical analysis showed that room conditions strongly influenced the distribution of airborne microorganisms and were also affected by indoor airflow patterns. The findings revealed higher concentrations in Room B than in Room A (morning averages: 60.75 vs. 23.75 CFU/m³; afternoon averages: 44.5 vs. 24.25 CFU/m³), with a statistically significant difference (Mann–Whitney, p-value < 0.05). These results indicate that optimizing airflow direction, scheduling cleaning activities, and conducting routine bioaerosol monitoring are necessary as indicators of control performance. Morphological analysis identified Gram-positive bacteria such as Staphylococcus and Bacillus, as well as fungi such as Aspergillus and Trichoderma, all of which may affect health and indoor air quality. This study underscores the importance of laboratory indoor air quality management in reducing health risks associated with airborne microorganisms.
Co-Authors Lathifa, Annisa Nur Prawitasari, Diah Ayu Rahmawati, Suphia