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All Journal Indonesian Journal of Applied Research (IJAR)
Deden Sudrajat
Department of Animal Science, Universitas Djuanda, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT

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Effectiveness of Diets Incorporating Nano-Zeolite and Urea-Impregnated Nano-Zeolite on Fermentation Efficiency in Rumen Fluid In Vitro Dede Kardaya; Pia Nurmala Dewi; Deden Sudrajat; Dewi Wahyuni; Ikhsan Qodri Pramartaa; Ruslan Abdul Gopar; Windu Negara; Raden Siti Nurlaela
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 3 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i3.767

Abstract

Zeolite had been widely investigated in animal feed for its ability to improve feed efficiency and reduce ammonia emissions, and nano-zeolite offered greater adsorption capacity due to its expanded surface area. This study evaluated the effects of nano-zeolite supplementation on ammonia (NH₃), volatile fatty acids (VFA), the VFA/NH₃ ratio, and pH during in vitro rumen fermentation. A two-factor randomized block design (6 × 6) with four replicates was used. Incubation time (2, 4, 8, 12, 24, and 48 h) served as the first factor, and six dietary treatments served as the second factor: control, urea-supplemented concentrate, inactivated nano-zeolite, activated nano-zeolite, urea-impregnated inactivated nano-zeolite, and urea-impregnated activated nano-zeolite. Nano-zeolite, whether active or urea-impregnated, did not significantly improve rumen fermentation efficiency based on NH₃, VFA, or VFA/NH₃ ratio. All nano-zeolite diets performed similarly to the control and urea-only diets. Fermentation efficiency peaked at 4 and 8 hours of incubation, indicating that nano-zeolite combined with urea supported stable fermentation, particularly during early to mid-incubation.
Co-Authors Dede Kardaya Dewi Wahyuni Ikhsan Qodri Pramartaa Pia Nurmala Dewi Raden Siti Nurlaela Ruslan Abdul Gopar Windu Negara