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Nuramaliah Desliani Putri
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Protective Effects of L-Carnitine on Growth and Cardiac Function in High-Glucose-Exposed Zebrafish Embryos Editya Fukata; Anditri Weningtyas; Lintang Widya Sishartami; Nuramaliah Desliani Putri; Putri Kusuma C. G. R. Asfandi
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.879-885

Abstract

Diabetes mellitus is a chronic metabolic disorder, and maternal hyperglycaemia during pregnancy increases the risk of fetal developmental complications. This study aimed to evaluate the protective role of L-carnitine against glucose-induced embryotoxicity in zebrafish (Danio rerio). Fertilized embryos were exposed to 5% glucose with or without L-carnitine supplementation (50 or 100 uM). Developmental outcomes including survival, hatching, malformations, body length, and heart rate were assessed using microscopy and analyzed by ANOVA and Kaplan–Meier survival curves. High glucose significantly reduced survival (hazard ratio = 6.86; p < 0.0001), delayed hatching (median hatching time 84 vs. 60 hpf; HR = 0.17, 95% CI = 0.08–0.39; p < 0.0001), and induced growth retardation and bradycardia (p < 0.0001). L-carnitine did not rescue survival or hatching but partially ameliorated growth impairment (p < 0.05) and restored heart rate in a dose-dependent manner, with 100 uM supplementation approaching control values (p < 0.001). Morphological abnormalities such as yolk sac edema and spinal curvature remained present but less severe with co-treatment. In conclusion, L-carnitine confers partial protective effects on growth and cardiac function under hyperglycaemic stress, although it does not prevent early lethality.
Peningkatan Kapasitas Kader Kesehatan dalam Penanganan Kegawatdaruratan melalui Edukasi Pertolongan Pertama Berbasis Usia Karina Nilasari; Putri Kusuma Cahyaning Gusti Rohmatillah Asfandi; Nuramaliah Desliani Putri; Syarifa Nadia
PaKMas: Jurnal Pengabdian Kepada Masyarakat Vol 6 No 1 (2026): Mei 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/pakmas.v6i1.5770

Abstract

Health cadres serve as vital front-liners in primary healthcare, especially as first responders when medical emergencies occur within the community. Yet, many of them still lack the necessary knowledge and confidence to deliver appropriate first aid, which can lead to delayed or incorrect actions. This community engagement program was designed to strengthen their capacity through age-specific first aid education tailored for infants, children, adolescents, adults, and the elderly. The training took place at Bumiaji Public Health Center, Batu City, and involved 80 selected health cadres. Conducted over one day, the program combined interactive lectures, demonstrations, group discussions, and pre- and post-training evaluations. The results showed a significant improvement in knowledge, with the mean error score decreasing from 5.66 to 1.94 (p < 0.001). Participants also expressed increased confidence in recognizing emergency signs and providing timely interventions. These findings highlight that even short, well-structured, and contextually relevant educational activities can substantially enhance community health workers’ readiness to act during emergencies. Replicating such programs in other regions could help strengthen community-based emergency preparedness and contribute to broader efforts in building resilient and responsive primary healthcare systems in Indonesia.
Co-Authors Anditri Weningtyas Editya Fukata Karina Nilasari Lintang Widya Sishartami Putri Kusuma C. G. R. Asfandi Putri Kusuma Cahyaning Gusti Rohmatillah Asfandi Syarifa Nadia