Article Per Year (5 Year)
p-Index From 2021 - 2026
0.562
P-Index
This Author published in this journals
All Journal African Journal of Biochemistry and Molecular Biology Research Open Access DRIVERset African Journal of Clinical Medicine and Pharmacy Research
Onwubiko G. N.
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Phytochemical and Anti-Sickling Properties of Aqueous Extract of Pennisetum purpureum Schumach (Elephant Grass) Shoots Onwubiko U. I.; Imo C.; Onwubiko G. N.; Boyi R.N; Dawoye Y.; Galadima A. O.; Sanusi A.; Solomon J. E.
African Journal of Biochemistry and Molecular Biology Research Vol 3 No 2 (2026): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v3i2.10474

Abstract

Sickle cell disease remains a major haematological disorder associated with haemoglobin S polymerization and erythrocyte sickling, highlighting the need to explore plant-derived compounds with potential anti-sickling activity. This study aimed to determine the anti-sickling potential of the aqueous extract of young shoots of Pennisetum purpureum using microscopy after 2% sodium metabisulphite-induced sickling of sickle red blood cells. The findings showed that Pennisetum purpureum extract significantly reversed many sickled cells to normal-shaped cells, with the percentage reversal depending on both concentration and exposure time. At 250 μg/mL, sickle cell reversal was 88.82% at 0 min, 86.09% at 30 min, and 85.19% at 60 min, whereas the control without extract showed no reversal. At 500 μg/mL, the reversal percentages were 80.14% at 0 min, 92.27% at 30 min, and 90.51% at 60 min. At 1000 μg/mL, reversal increased from 79.13% at 0 min to 94.82% at 30 min and 96.50% at 60 min. Phytochemical analysis indicated the presence of aromatic and alkylic compounds, including quercetin, epicatechin, resveratrol, vanillic acid, ellagic acid, and kaempferol. These compounds may contribute to anti-sickling activity through hydrophobic interactions that interfere with haemoglobin S polymerization and stabilize the hydrophobic erythrocyte lipid bilayer membrane. This study contributes to phytomedicine and sickle cell research by demonstrating the potential of Pennisetum purpureum young shoot extract as a source of bioactive compounds with anti-sickling properties, although further biochemical, toxicological, and clinical validation is required.
Phytochemical and Anti-Sickling Properties of Aqueous Extract of Pennisetum purpureum Schumach (Elephant Grass) Shoots Onwubiko U. I.; Imo C.; Onwubiko G. N.; Boyi R.N; Dawoye Y.; Galadima A. O.; Sanusi A.; Solomon J. E.
African Journal of Biochemistry and Molecular Biology Research Vol 3 No 2 (2026): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v3i2.10474

Abstract

Sickle cell disease remains a major haematological disorder associated with haemoglobin S polymerization and erythrocyte sickling, highlighting the need to explore plant-derived compounds with potential anti-sickling activity. This study aimed to determine the anti-sickling potential of the aqueous extract of young shoots of Pennisetum purpureum using microscopy after 2% sodium metabisulphite-induced sickling of sickle red blood cells. The findings showed that Pennisetum purpureum extract significantly reversed many sickled cells to normal-shaped cells, with the percentage reversal depending on both concentration and exposure time. At 250 μg/mL, sickle cell reversal was 88.82% at 0 min, 86.09% at 30 min, and 85.19% at 60 min, whereas the control without extract showed no reversal. At 500 μg/mL, the reversal percentages were 80.14% at 0 min, 92.27% at 30 min, and 90.51% at 60 min. At 1000 μg/mL, reversal increased from 79.13% at 0 min to 94.82% at 30 min and 96.50% at 60 min. Phytochemical analysis indicated the presence of aromatic and alkylic compounds, including quercetin, epicatechin, resveratrol, vanillic acid, ellagic acid, and kaempferol. These compounds may contribute to anti-sickling activity through hydrophobic interactions that interfere with haemoglobin S polymerization and stabilize the hydrophobic erythrocyte lipid bilayer membrane. This study contributes to phytomedicine and sickle cell research by demonstrating the potential of Pennisetum purpureum young shoot extract as a source of bioactive compounds with anti-sickling properties, although further biochemical, toxicological, and clinical validation is required.
The Anti-Sickling and Oxidative Properties of Ethanol Leaf Extract of Ocimum gratissimum L. (Scent Leaf) Onwubiko U. I.; Imo C.; Onwubiko G. N.; Dawoye Y.; Rayyanu S. S.; Solomon J. E.
African Journal of Clinical Medicine and Pharmacy Research Vol 3 No 2 (2026): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajcmpr.v3i2.9092

Abstract

This study evaluated the anti-sickling potential of the ethanol leaf extract of Ocimum gratissimum using microscopy-based assays. The extract markedly reversed 2% sodium metabisulfite–induced sickled red blood cells to normal biconcave forms, and microscopic observations indicated that the percentage reversal of sickled cells was both concentration- and time-dependent. At 250 μg/mL, the percentage reversal of sickled cells at 0, 20, and 30 minutes was 77.00%, 72.31%, and 71.77%, respectively, whereas the control (absence of extract) showed no reversal. At 500 μg/mL, the corresponding reversals were 76.19%, 69.60%, and 74.00%, while at 1000 μg/mL they were 71.49%, 75.92%, and 87.70%, respectively, demonstrating enhanced anti-sickling activity at higher extract concentrations over time. UV–Visible spectrophotometric analysis of oxyhaemoglobin revealed a gradual increase in the 630 nm band, consistent with the oxidation of ferrous (Fe²⁺) oxyhaemoglobin to ferric (Fe³⁺) methaemoglobin; the absorbance peak at 650 nm, attributable to chlorophyll, was clearly distinguishable from the haemoglobin oxidation band at 630 nm. In addition, the ethanol leaf extract of O. gratissimum exhibited a stabilizing effect on erythrocyte haemolysis. Phytochemical analysis showed the presence of aromatic and alkylic compounds, which may contribute to intracellular anti-sickling activity by interfering with haemoglobin S polymerization and, through their hydrophobic properties, may also modulate the erythrocyte lipid bilayer membrane.
Co-Authors Boyi R.N Dawoye Y. Galadima A. O. Imo C. Onwubiko U. I. Rayyanu S. S. Sanusi A. Solomon J. E.