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All Journal Jurnal Purifikasi Bioscientist : Jurnal Ilmiah Biologi Open Community Service Journal Indonesian Journal of Community Services Cel Panthera : Jurnal Ilmiah Pendidikan Sains dan Terapan Jurnal Rekayasa Infrastruktur Hexagon
Diah Ayu Prawitasari
Jurusan Teknik Lingkungan, Universitas Islam Indonesia, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Social Sciences Other

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Analysis of the Abundance and Morphological Characteristics of Airborne Microorganisms in an Environmental Engineering Laboratory Diah Ayu Prawitasari; Rina Isnikarita; Annisa Nur Lathifa; Suphia Rahmawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.17342

Abstract

This study aimed to analyze the abundance and morphology of airborne microorganisms in the Environmental Engineering Laboratory and to relate their distribution to differences in room conditions and sampling time. Sampling was conducted in two rooms, Room A and Room B, during the morning and afternoon using a microbiological air sampler with Nutrient Agar (NA), Potato Dextrose Agar (PDA), and Plate Count Agar (PCA) media. Statistical analysis showed that room conditions strongly influenced the distribution of airborne microorganisms and were also affected by indoor airflow patterns. The findings revealed higher concentrations in Room B than in Room A (morning averages: 60.75 vs. 23.75 CFU/m³; afternoon averages: 44.5 vs. 24.25 CFU/m³), with a statistically significant difference (Mann–Whitney, p-value < 0.05). These results indicate that optimizing airflow direction, scheduling cleaning activities, and conducting routine bioaerosol monitoring are necessary as indicators of control performance. Morphological analysis identified Gram-positive bacteria such as Staphylococcus and Bacillus, as well as fungi such as Aspergillus and Trichoderma, all of which may affect health and indoor air quality. This study underscores the importance of laboratory indoor air quality management in reducing health risks associated with airborne microorganisms.
Akselerasi Kesiapan PROKLIM Lestari Melalui Pendampingan Partisipatif Berbasis Nilai Lokal di Kalurahan Banguntapan Ikrom Mustofa; Aiko Sarasvaty Prabowo; Diah Ayu Prawitasari; Dewi Wulandari; Ibnu Darmawan; Wahda Annisa Nuurussyahba; Ahmmad Azamuddin; Silvia Zalda Putri; Jihan Nanda Fitriyani
Open Community Service Journal Vol. 5 No. 1 (2026): Open Community Service Journal
Publisher : Research and Social Study Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33292/ocsj.v5i1.197

Abstract

Latar Belakang: Perubahan iklim menjadi tantangan global yang berdampak signifikan pada sistem sosial, ekonomi, dan lingkungan, khususnya di wilayah perkotaan dan peri-urban. Program Kampung Iklim (PROKLIM) merupakan inisiatif berbasis masyarakat yang dikembangkan oleh pemerintah Indonesia untuk meningkatkan kapasitas adaptasi dan mitigasi perubahan iklim di tingkat lokal. Namun demikian, implementasi PROKLIM di tingkat padukuhan masih menghadapi berbagai tantangan, terutama terkait kapasitas kelembagaan, dokumentasi kegiatan, serta integrasi program lintas sektor.Tujuan: Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan kesiapan padukuhan di Kalurahan Banguntapan, Kapanewon Banguntapan, Kabupaten Bantul, Daerah Istimewa Yogyakarta, Indonesia dalam kategori PROKLIM Lestari melalui pendekatan pendampingan partisipatif.Metode: Pelatihan peningkatan kapasitas, penilaian baseline pada 11 padukuhan, serta pendampingan implementasi aksi adaptasi dan mitigasi berbasis masyarakat.Hasil: Hasil penilaian baseline menunjukkan bahwa dari 11 padukuhan yang dinilai, 1 padukuhan berada pada kategori kesiapan tinggi (Padukuhan Plumbon), 6 padukuhan berada pada kategori kesiapan sedang, dan 4 padukuhan berada pada kategori kesiapan rendah, terutama pada aspek dokumentasi kegiatan dan kelembagaan PROKLIM. Intervensi berupa penguatan kelembagaan, penyusunan dokumentasi kegiatan, penerapan teknologi sederhana seperti komposter, lubang biopori, dan pemanenan air hujan terbukti meningkatkan kapasitas masyarakat dalam menghadapi perubahan iklim serta mempercepat kesiapan menuju PROKLIM Lestari. Kolaborasi akademisi, organisasi masyarakat sipil, pemerintah kalurahan, dan sektor swasta menjadi faktor kunci dalam keberhasilan program pendampingan. Kegiatan ini berkontribusi terhadap penguatan implementasi PROKLIM di tingkat lokal serta mendukung pencapaian Sustainable Development Goals (SDGs) dan Nationally Determined Contributions (NDC) Indonesia.
Implementasi Environmental Education Day: Water, Climate, and Sustainability di SDN Sardonoharjo 1 melalui Hands-On dan Game-Based Learning untuk Membangun Literasi Iklim Berkelanjutan Diah Ayu Prawitasari; Wahda Annisa Nuurussyahba; Ikrom Mustofa
Panthera : Jurnal Ilmiah Pendidikan Sains dan Terapan Vol. 5 No. 4 (2025): October
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/panthera.v5i4.757

Abstract

The Environmental Education Day: Water, Climate, and Sustainability activity at SDN Sardonoharjo 1 was implemented to foster sustainable climate literacy among elementary school students through an interactive and contextual learning approach. This activity employed a descriptive qualitative method with a single case study design, involving fourth, fifth, and sixth-grade students in a series of hands-on and game-based learning activities tailored to their developmental levels. The results show that this learning model significantly improved students’ understanding of water and climate concepts through concrete and enjoyable learning experiences. Students demonstrated enhanced ability to relate the hydrological cycle and climate change concepts to real-world phenomena and logically identify the causes and impacts of climate change. Furthermore, the activity strengthened 21st-century skills such as critical thinking, communication, collaboration, and creativity, as evidenced by active participation, problem-solving ability, and the quality of student-produced posters. Other findings indicate an increase in ecological awareness and environmentally friendly behavior, reflected in students’ reflective attitudes during games and classroom discussions. This program proved effective in instilling sustainability values from an early age and can serve as a replicable learning model for strengthening environmental education at the elementary school level.
Stakeholder Analysis of a Climate-Smart Agroforestry Community Service Program in the Dieng Highlands Ikrom Mustofa; Dhandhun Wacano; Diah Ayu Prawitasari; Akhmad Irfan; Niesa Hanum Mistoro
Indonesian Journal of Community Services Cel Vol. 5 No. 2 (2026): Indonesian Journal of Community Services Cel
Publisher : Research and Social Study Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70110/ijcsc.v5i2.149

Abstract

Background: Highland farming in the Dieng landscape faces land degradation and climate uncertainty. Technical recommendations may not endure when authority, work, and benefits are not negotiated among local actors.Aims: This community-service evaluation mapped stakeholder interests, influence, participation, and relationships in a climate-smart agroforestry program in Serang Village, Central Java.Methods: Activities were held on 4–5 October 2025, with 18 attendance records on the first day and 19 on the second. Evidence came from documents, field observation, group dialogue, semi-structured conversations, and UII–UNSIQ team validation.Result: Stakeholders were assessed through a power–interest matrix, salience attributes, and the IAP2 participation spectrum. Farmer groups, UII, and UNSIQ emerged as key players; the village government as a context setter; and KWT/PKK and students as interested actors with limited decision power. Participation remained at involve–collaborate rather than empowerment. Priorities are a joint demonstration plot, stronger KWT/PKK decision roles, village coordination, shared monitoring, and selective engagement with extension and government. The findings also show that combining power–interest, stakeholder salience, and participation analysis can reveal differences in influence that may not be visible from attendance or participation records alone.
Hubungan Indeks Kualitas Air Tanah dengan Jumlah dan Kepadatan Penduduk di Yogyakarta Ihsan Fauzi Shadiqi; Any Juliani; Diah Ayu Prawitasari
Jurnal Rekayasa Infrastruktur HEXAGON Vol 10 No 1 (2025): Edisi Juni
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Air tanah adalah sumber daya air dengan kuantitas dan kualitas terjamin dimana sekitar 30,1% air dari sumber daya air tawar yang ada di bumi merupakan air tanah. Tingginya kualitas air tanah didukung oleh formasi geologi yang secara alami menyaring dan memperkaya kandungan mineral dalam air tanah. Seiring berjalan waktu, kuantitas dan kualitas ini dapat dipengaruhi oleh peningkatan pertumbuhan penduduk yang bermuara pada peningkatan akitivtas penduduk dan permintaan akan air bersih, terutama di daerah perkotaan. Kota Yogyakarta mengalami pertumbuhan penduduk yang pesat dengan kepadatan penduduk berjumlah 212.282 jiwa/km2 pada tahun 2019 di luasan areanya sebesar 32,5 km2. Peningkatan jumlah dan kepadatan penduduk pada tahun 2019 hingga tahun 2022 mengakibatkan Water Quality Index (WQI) meningkat atau kualitas air menjadi menurun. Water Quality Index (WQI) adalah metode penilaian kualitas air berdasarkan parameter fisika dan kimia. Hubungan antara peningkatan jumlah penduduk dan WQI dianalisis melalui metode uji korelasi. Didapatkan bahwa hasil uji korelasi di tahun 2019 sebesar -0,430; -0,202 di tahun 2020; -0,205 di tahun 2021; -0,207 di tahun 2022; sementara pada tahun 2023 terdapat peningkatan penduduk namun terjadi penurunan WQI dengan nilai korelasi sebesar 0,102. Perbedaan ini menunjukkan kemungkinan peningkatan proses pegelolaan air yang lebih efektif pada tahun ini. Secara keseluruhan, nilai-nilai hasil uji korelasi mengindikasikan adanya kemerosotan kualitas air tanah akibat meningkatnya kepadatan penduduk. Hal ini kemudian menggarisbawahi kebutuhan mendesak akan strategi pengelolaan air tanah berkelanjutan untuk memastikan ketersediaan dan kontinuitas air yang aman bagi masyarakat.
EXTRACTION OF LACCASE ENZYME PHANEROCHAETE CHRYSOSPORIUM IN RICE HUSK MEDIA FOR TOTAL PETROLEUM HYDROCARBON REDUCTION Diah Ayu Prawitasari; Annisa Muthmainah
Purifikasi Vol 25 No 2 (2026): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Planning, and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/purifikasi.v25i2.536

Abstract

Total Petroleum Hydrocarbon (TPH) is a persistent soil contaminant that requires effective and sustainable remediation strategies. This study investigates the production and application of crude laccase enzyme from Phanerochaete chrysosporium cultivated using rice husk as a low-cost lignocellulosic substrate for TPH reduction in contaminated soil. Fungal cultures were initially propagated on PDA medium and subsequently transferred to PDB supplemented with rice husk under controlled conditions of 30°C and pH 7. Crude laccase was extracted using phosphate buffer, and its enzymatic activity was measured spectrophotometrically using ABTS as a substrate. After 14 days of incubation, laccase activity reached 24.49 U/L, with a maximum crude enzyme yield of 37.42%. The application of 15 mL of crude laccase extract to 5 g of contaminated soil reduced TPH concentration from 10% to 4.67%, achieving a removal efficiency of 53.3%. Laccase facilitates hydrocarbon degradation through oxidation reactions, particularly targeting aromatic and phenolic compounds. These findings demonstrate the potential of rice husk as an economical substrate for fungal laccase production and support the application of crude laccase as a promising approach for sustainable soil bioremediation. Further optimization of enzyme production and treatment conditions is recommended to enhance remediation efficiency.
Co-Authors Ahmmad Azamuddin Aiko Sarasvaty Prabowo Akhmad Irfan Annisa Muthmainah Annisa Nur Lathifa Any Juliani, Any Dewi Wulandari Dhandhun Wacano Ibnu Darmawan Ihsan Fauzi Shadiqi Ikrom Mustofa Jihan Nanda Fitriyani Niesa Hanum Mistoro Rahmawati, Suphia Rina Isnikarita Silvia Zalda Putri Wahda Annisa Nuurussyahba Wahda Annisa Nuurussyahba