Raka Selaksa Charisma Muchammad
Universitas Pembangunan Nasional “Veteran” Jawa Timur, Indonesia

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Effect of Fermentation Time and Lactobacillus plantarum Inoculum Concentration on Lactic Acid Production from Durian Peel Waste Nanda Yunita Pratiwi; Shintya Hotnauli Turnip; Sani Sani; Raka Selaksa Charisma Muchammad; Aisyah Alifatul Zahidah Rohmah; Suprihatin Suprihatin
G-Tech: Jurnal Teknologi Terapan Vol 10 No 2 (2026): G-Tech, Vol. 10 No. 2 April 2026
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v10i2.9425

Abstract

Lactic acid is an important chemical whose demand continues to increase every year. Therefore, alternative innovations are needed to meet this demand. One potential raw material that can be utilized is durian peel waste, which is high in cellulose and can be converted into reducing sugar through pretreatment and hydrolysis as a fermentation substrate to produce lactic acid. This study aims to determine the optimal conditions for fermentation time (5 hours; 6.5 hours; 8 hours; 9.5 hours and 11 hours) and Lactobacillus Plantarum inoculum concentration (5%, 10%, 15%, 20%, 25%) in producing high concentrations of lactic acid. The research was conducted through Alkali Hydrogen Peroxide (AHP) delignification, acid hydrolysis, and fermentation processes. The lactic acid content was analyzed using the acid-base titration method (Total Titratable Acidity). The results showed that the highest lactic acid content was obtained at a fermentation time of 11 hours with an inoculum concentration of 20%, which reached 0.135%. Thus, fermentation time and inoculum concentration affect the lactic acid content produced, and durian peel waste has the potential to be used as an alternative raw material in lactic acid production.
Alkali Hydrogen Peroxide (AHP) Pretreatment to Increase The Cellulose Content In Durian Peel Waste Nur Makhmudi; Tri Utami Setyawati; Sani Sani; Aisyah Alifatul Zahidah Rohmah; Raka Selaksa Charisma Muchammad
G-Tech: Jurnal Teknologi Terapan Vol 10 No 3 (2026): G-Tech, Vol. 10 No. 3 July 2026
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v10i3.9675

Abstract

Indonesia is one of the largest producers of durian, generating substantial quantities of durian peel waste. Due to its high cellulose content, durian peel has strong potential as a lignocellulosic feedstock for reducing sugar and biofuel production. However, the presence of lignin and hemicellulose limits cellulose accessibility and inhibits hydrolysis. This study investigated Alkali Hydrogen Peroxide (AHP) pretreatment to enhance cellulose purity in durian peel waste. Pretreatment was carried out using H₂O₂ concentrations of 0.5–8.5% (w/v) with NaOH adjustment to pH 11–12 at 70°C for 3 h. The optimum condition was obtained at 8.5% H₂O₂, which increased cellulose content from 63.02% to 75.05%, reduced hemicellulose by 24.4%, and removed 99.5% of lignin. Scanning Electron Microscopy (SEM) analysis confirmed the formation of a rougher and more porous surface after pretreatment, indicating disruption of the lignocellulosic structure. Statistical analysis using ANOVA showed that H₂O₂ concentration significantly affected cellulose, hemicellulose, and lignin responses (p < 0.05). These results demonstrate that AHP pretreatment is highly effective in improving cellulose accessibility and provides a promising approach for converting durian peel waste into reducing sugars and other bio-based products.