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Tjie Jan Tan
Universitas Pelita Harapan
Computer Science & IT Environmental Science

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PENGOMPOSAN SAMPAH ORGANIK DAN ISOLASI BAKTERI TERMOFIL DARI KOMPOS [COMPOSTING ORGANIC WASTE AND ISOLATING THERMOPHILE BACTERIA FROM COMPOST] Tjie Jan Tan; Juan Daniel; Hans Victor; Marcelia Sugata
FaST : Jurnal Sains dan Teknologi Vol. 7 No. 2 (2023): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v7i2.7596

Abstract

Composting is one of the methods to sustainably minimize the accumulation of waste in the environment. Compost is made through the decomposition process of organic waste with the help of microorganisms. The process of making compost goes through several phases, one of the most important phases is the thermophilic phase. In this research, an evaluation was carried out on the effect of temperature, humidity, pH and aeration on the success of composting from leaves and twigs and braches of trembesi tree (Albizia saman). In addition, isolation and identification of thermophilic bacteria which play an important role in the composting process were carried out. Isolation of thermophilic bacteria is carried out when the compost making process is in the thermophilic phase. To support the growth of thermophilic bacteria, incubation during the isolation and purification process was carried out at a temperature of as high as 55-60 oC. Subsequently, thermophilic bacterial isolates were identified based on morphology and biochemical activity tests. The characterization results based on Bergey's Manual Systematic Bacteriology Second Edition Volume Three the Firmicutes indicated that the isolates obtained belonged to the genus Bacillus.Bahasa Indonesia Abstract:Pembuatan kompos merupakan salah satu cara yang dapat dilakukan untuk meminimalisasi penumpukan sampah di lingkungan secara berkelanjutan. Kompos dibuat melalui proses dekomposisi sampah organik dengan bantuan mikroorganisme. Proses pembuatan kompos melalui beberapa fase, salah satu fase terpenting adalah fase termofilik. Pada penelitian ini, dilakukan evaluasi pengaruh suhu, kelembapan, pH dan aerasi terhadap keberhasilan pembuatan kompos dari daun dan ranting serta dahan pohon trembesi (Albizia saman) Selain itu, dilakukan isolasi dan identifikasi bakteri termofilik yang berperan penting dalam proses pengomposan. Isolasi bakteri termofilik dilakukan saat proses pembuatan kompos berada pada fase termofilik. Untuk mendukung pertumbuhan bakteri termofil, inkubasi selama proses isolasi dan purifikasi dilakukan pada suhu 55-60 oC. Selanjutnya, isolat bakteri termofil diidentifikasi berdasarkan morfologi dan uji aktivitas biokimia. Hasil karakteriasasi yang dicocokan dengan Bergey’s Manual Systematic Bacteriology Second Edition Volume Three the Firmicutes mengindikasikan bahwa isolat yang diperoleh berasal dari genus Bacillus.
Evaluation of Plantaricin Gene Expression in Lactiplantibacillus plantarum Strain SU-KC1a: [Evaluasi Ekspresi Gen Plantarisin pada Lactiplantibacillus plantarum Strain SU-KC1a] Marcelia Sugata; Jessica Sunardi; Hans Victor; Tjie Jan Tan
FaST : Jurnal Sains dan Teknologi Vol. 9 No. 1 (2025): MAY
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v9i1.9595

Abstract

Lactobacillus species are normal gastrointestinal flora and are commonly used in the food industry for fermented milk products. Due to their probiotic properties, Lactobacillus strains are considered safe and effective for human consumption, with antimicrobial activity often attributed to bacteriocins. Lactobacillus plantarum (known as Lactiplantibacillus plantarum) produces plantaricin, a ribosomally synthesized bacteriocin with activity against a range of pathogens and potential as a bio-preservative. In this study, L. plantarum SU-KC1a, isolated from human breast milk, was assessed for its antimicrobial activity through the production of plantaricin. Since plantaricin is typically produced at low concentrations, reverse transcription polymerase chain reaction (RT-PCR) was employed. PCR amplification of the plnJ and plnK genes resulted in amplicons of approximately 168 bp and 173 bp, respectively. These findings suggest that L. plantarum SU-KC1a produces plantaricin JK. However, further sequence analysis is required to confirm the homology of the amplified genes with known plantaricin J and K genes in GenBank. Bahasa Indonesia Abstract: Spesies Lactobacillus adalah flora gastrointestinal normal dan sering digunakan dalam industri makanan untuk produk susu fermentasi. Karena sifat probiotiknya, strain Lactobacillus dianggap aman dan efektif untuk konsumsi manusia, dengan aktivitas antimikroba yang sering dikaitkan dengan bakteriosin. Lactobacillus plantarum (dikenal sebagai Lactiplantibacillus plantarum) menghasilkan plantarisin, sebuah bakteriosin yang disintesis ribosom dengan aktivitas melawan berbagai patogen dan berpotensi sebagai bio-preservatif. Dalam penelitian ini, L. plantarum SU-KC1a yang diisolasi dari ASI manusia dievaluasi aktivitas antimikrobanya melalui produksi plantarisin. Karena plantarisin biasanya diproduksi dalam konsentrasi rendah, dilakukan reverse transcription polymerase chain reaction (RT-PCR). Amplifikasi PCR dari gen plnJ dan plnK menghasilkan produk amplifikasi sekitar masing-masing 168 bp dan 173 bp. Hasil tersebut mengindikasikan bahwa L. plantarum SU-KC1a menghasilkan plantarisin JK. Namun, analisis urutan lebih lanjut diperlukan untuk mengonfirmasi kesamaan gen yang diamplifikasi dengan gen plantarisin J dan K yang diketahui di GenBank.
Co-Authors Jessica Sunardi Juan Daniel Sugata, Marcelia Victor, Hans