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All Journal Jurnal Biogenerasi
Yusuf Nasution, Mhd
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Education Languange, Linguistic, Communication & Media Other

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Kode Etik Pengelolaan Limbah Bekas Dan Bahan Terkontaminasi Pada Laboratorium Kultur Jaringan Tanaman Athifah, Najwa Athifah; Sani, Ribi Zuhriah Sani; Yusuf Nasution, Mhd; Lubis, Khairiza; Situmorang, Nurbaity
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/rabwff67

Abstract

This study aims to describe the management of used culture media waste and contaminated materials, and to assess the understanding of laboratory staff and interns regarding ethical principles in plant tissue culture laboratories. A descriptive qualitative method was applied using observation, questionnaires, and literature review. The results indicate that most respondents have a good understanding, reflected in their agreement on the importance of sterilization, waste segregation, use of personal protective equipment, and implementation of standard operating procedures (SOP). Respondents are also aware of the risks of laboratory waste to health and the environment. Ethical practices are recognized as part of professional responsibility, but require improvement through training, supervision, and consistent SOP implementation.  
Penerapan Prinsip Bioetik dalam Pengujian Escherichia coli pada Air dan Salmonella pada Ikan Nila (Oreochromis niloticus) Menggunakan Media Compact Dry Berdasarkan Standar ISO/IEC 17025 di Laboratorium BPPMHKP Medan I Andina Ayu Syahputri; Dara Arifia, Dini; Situmorang, Nurbaity; Yusuf Nasution, Mhd; Arwita, Widya
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/xk28gj62

Abstract

Food safety and environmental quality are critical aspects in maintaining public health, particularly in fishery products and water sources that are vulnerable to contamination by pathogenic microorganisms. This study aims to identify the presence of Escherichia coli in water samples and Salmonella in tilapia (Oreochromis niloticus), as well as to analyze the use of Compact Dry media based on ISO/IEC 17025 and the implementation of bioethical principles in microbiological testing. This research employed a descriptive laboratory approach conducted at the BPPMHKP Medan I Laboratory using Compact Dry media based on AOAC methods. The samples consisted of lake water and tilapia, which were tested through enrichment, inoculation, incubation, and colony observation stages. The results showed no growth of Escherichia coli colonies on Compact Dry EC media and no Salmonella colonies on Compact Dry SL media, indicating that all samples were negative for the tested bacteria. The use of Compact Dry media in this study complied with ISO/IEC 17025 standards, as indicated by systematic procedures, the use of validated media, and laboratory quality control. Furthermore, bioethical principles, including scientific integrity, responsibility, biosafety, and accountability, were applied throughout all stages of testing. Therefore, this study demonstrates that the use of validated media and the application of bioethical principles play an important role in ensuring valid, reliable, and accountable laboratory results.
Co-Authors Andina Ayu Syahputri Athifah, Najwa Athifah Dara Arifia, Dini Lubis, Khairiza Sani, Ribi Zuhriah Sani Situmorang, Nurbaity Widya Arwita