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All Journal International Journal of Quantitative Research and Modeling
Muttaqin, Asep Zainal
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Computer Science & IT Decision Sciences, Operations Research & Management Engineering Environmental Science Physics

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The Effect Of Addition Of Benzyl Amino Purine (BAP) and Bean Sprout Extract in Murashige and Skoog (MS) On The Growth Of Hybrid Cattleya Orchid Tissue Culture In Vitro Dewi, Elena Fitria; Budiono, Ruly; Muttaqin, Asep Zainal
International Journal of Quantitative Research and Modeling Vol. 7 No. 1 (2026): International Journal of Quantitative Research and Modeling
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijqrm.v7i1.1293

Abstract

Cattleya orchids are highly sought-after ornamental plants, leading to the development of hybrid Cattleya orchids. However, conventional propagation of Cattleya orchids is considered difficult. Therefore, in vitro tissue culture is necessary as an alternative. Important factors in the success of tissue culture are the growing medium and plant growth regulators (PGRs), such as cytokinins and auxins. This study aimed to examine the effect of the addition of synthetic PGRs (BAP) and natural PGRs (bean sprout extracts) combined on the growth of hybrid Cattleya orchid tissue cultures in vitro. This study was conducted using a Completely Randomized Design experimental method with 5 levels of BAP concentration (0 ppm, 0.5 ppm, 1 ppm, 2 ppm, 3 ppm), bean sprout extract (0%, 5%, 7.5%, 10%, 12.5%) and a combination of BAP and bean sprout extract, namely control, BAP 0.5 ppm + 5% bean sprout extract, BAP 1 ppm + 7.5% bean sprout extract, BAP 2 ppm + 10% bean sprout extract, BAP 3 ppm + 12.5% ​​bean sprout extract on MS media. The parameters observed included plantlet height, number of leaves, number of roots, root length, and fresh weight. Data were analyzed using analysis of variance (ANOVA) and continued with Duncan's test. The results showed that the administration of BAP, bean sprout extract and their combination had a significant effect on the growth of plantlet height, number of leaves, number of roots, root length and fresh weight. The combination treatment of 2 ppm BAP and 10% bean sprout extract was effective in supporting the growth of hybrid Cattleya orchids.
The Effect Of Tomato Extract (Solanum lycopersicum L.) and Coconut Water (Cocos nucifera L.) as Growth Regulators (PGR) in Murashige And Skoog (Ms) Media on the Growth of In Vitro Crossed Cattleya Orchid Tissue Culture Satiyanto, Hanny Putri; Budiono, Ruly; Muttaqin, Asep Zainal
International Journal of Quantitative Research and Modeling Vol. 7 No. 1 (2026): International Journal of Quantitative Research and Modeling
Publisher : Research Collaboration Community (RCC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijqrm.v7i1.1297

Abstract

Cattleya orchids are highly prized ornamental plants that can be significantly propagated using in vitro tissue culture techniques. PGRs significantly influences the success of tissue culture. Synthetic PGRs are commonly used, but are relatively expensive and not environmentally friendly, so natural materials need to be used as alternatives. The purpose of this study was to determine how tomato extract (Solanum lycopersicum L.), coconut water (Cocos nucifera L.), and their combination as natural PGRs affect the growth of hybrid Cattleya orchid plantlets on Murashige and Skoog (MS) media. A Completely Randomized Design (CRD) was used to treat tomato extract (0%; 1%; 1.5%; 2%; 2.5%) and coconut water (0%; 5%; 10%; 15%; 20%) and their combinations. This study analyzed data based on parameters of plantlet height, number of leaves, number of roots, root length, and wet weight using ANOVA analysis followed by Duncan's test at the 5% level. The results showed that several growth parameters were affected such as plantlet height, number of roots, root length, and fresh weight showed significant effects. The number of leaves did not have a significant effect. Natural PGRs are generally considered a more cost-effective and environmentally friendly alternative, their effectiveness has not completely replaced synthetic PGRs.
Co-Authors Dewi, Elena Fitria Ruly Budiono Satiyanto, Hanny Putri