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Antibacterial Activity of Moringa (Moringa oleifera L.) Leaf Extract Against Salmonella typhi: A Study for Monograph Development in Biology Education Efriyandika, Toni; Putra, Resna Hegi; Pantiwati, Yuni
ISEJ : Indonesian Science Education Journal Vol. 6 No. 2 (2025): May
Publisher : Yayasan Darussalam Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62159/isej.v6i2.2068

Abstract

Salmonella typhi, the causative agent of typhoid fever, has increasingly developed resistance to conventional antibiotics, necessitating research into alternative antimicrobial agents. Moringa oleifera L. is a medicinal plant rich in phytochemical compounds including flavonoids, saponins, tannins, and terpenoids with documented antibacterial properties. This study examined the antibacterial activity of ethanol extract of moringa leaves against S. typhi growth, and evaluated the validity and practicality of a monograph developed from these experimental findings for undergraduate microbiology education. Using a True Experimental Research design with a Completely Randomized Design (CRD), the antibacterial activity was assessed through the Kirby-Bauer disc diffusion method at concentrations of 12.5%, 25%, 50%, and 100%, with Aztreonam as positive control. The monograph development followed the 4-D model (Define, Design, Develop, Disseminate). Results demonstrated that all extract concentrations significantly inhibited S. typhi growth (p = 0.000), with the highest inhibition zone diameter of 14.18 mm at 100% concentration, classified as strong inhibition. Higher concentrations consistently produced larger inhibition zones, indicating a dose-dependent antibacterial effect. The developed monograph achieved validity scores of 91.67% (content expert) and 92.85% (media expert), both categorized as valid. Practicality testing with 14 students yielded a score of 87.93%, indicating the monograph is highly practical for use in microbiology courses. These findings confirm moringa leaf extract as a promising natural antibacterial agent and establish its viability as research-based educational material.
Menumbuhkan Kesadaran Akan Pentingnya Pendidikan Anak Usia Dini melalui Motivasi Belajar: Pendekatan Partisipatif dalam Pemberdayaan Komunitas Putra, Resna Hegi; Efriyandika, Toni; Pantiwati, Yuni; Hindun, Iin; Raharjdjanto, Abdulkadir
SAKALIMA: Pilar Pemberdayaan Masyarakat Pendidikan Vol. 2 No. 2 (2025): December | SAKALIMA: Pilar Pemberdayaan Masyarakat Pendidikan
Publisher : WISE Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70211/sakalima.v2i2.285

Abstract

This study aims to strengthen community awareness of the importance of early childhood education through a participatory learning motivation program facilitated by university students in Sukaraja Village, West Lampung, Indonesia. Using a descriptive qualitative design with a participatory approach, the research involved 15 children aged 4-6 years and 10 parents selected through purposive sampling. Data were collected through semi-structured interviews, field observations, and documentation, then analyzed using Miles and Huberman’s interactive model supported by triangulation to ensure validity. The findings demonstrate that participatory learning activities significantly enhanced children’s enthusiasm, parental involvement, and collective community awareness toward early education. The program successfully integrated intrinsic and extrinsic motivation by combining play-based methods, peer interaction, and contextual learning environments. Furthermore, two informal learning groups emerged as a sustainable community-driven initiative after the program ended. The study contributes a novel participatory motivation framework that integrates educational awareness, family engagement, and community empowerment to promote inclusive early education in rural contexts.