Jurnal Kelautan : Indonesian Journal of Marine Science and Technology
Vol 12, No 1 (2019)

PENGARUH KONSENTRASI PUPUK UREA TERHADAP PERTUMBUHAN POPULASI SEL Nannochloropsis sp.

Yuyun Arfah (Mataram University)
Nunik Cokrowati ([SCOPUS ID: 56974439100, h-index:1], Program Studi Budidaya Perairan, Universitas Mataram)
Alis Mukhlis (Mataram University)



Article Info

Publish Date
03 Jul 2019

Abstract

Nannochloropsis sp. merupakan mikroalga yang dibudidayakan sebagai pakan alami serta memiliki peranan penting dalam usaha pembenihan ikan, udang, kepiting dan biota laut lainnya. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi pupuk urea terhadap pertumbuhan populasi sel Nannochloropsis sp dan konsentrasi pupuk terbaik untuk pertumbuhan populasi sel Nannochloropsis sp. Metode yang digunakan dalam penelitian ini yaitu metode ekseprimen menggunakan Rancangan Acak Lengkap (RAL), dengan 6 perlakuan yaitu pemberian pupuk KW21 sebnyak 1 ml/L sebagai kontrol, 0 mg/L, 15 mg/L, 30 mg/L, 45 mg/L dan 60 mg/L yang diulang sebanyak 4 kali. Pengamatan kepadatan dilakukan selama 24 jam. Parameter yang diamati meliputi kepadatan populasi sel Nannochlorpsis sp., pertumbuhan relatif, waktu penggandaan diri dan pertumbuhan spesifik. Hasil penelitian menunjukan, bahwa kepadatan tertinggi populasi sel Nannochlorpsis sp. didapatkan pada konsentrasi pupuk urea 45 mg/L dengan kepadatan populasi sel sebesar 2,2625 juta sel/mL yang dicapai pada jam ke-24 setelah fase adaptasi. Dan pertumbuhan relatif tertinggi  50,83 %  dari kepadatan populasi awal, serta waktu penggandaan diri tersingkat yaitu 40,8 jam dengan pertumbuhan spesifik 0,8625 % per jam. Berdasarkan hasil penelitian ini, dianjurkan pada budidaya Nannochloropsis sp. untuk meningkatkan pertumbuhan populasi sel adalah pupuk urea dengan konsentrasi 45 mg/L.Kata kunci : pakan alami, budidaya, kepadatan, waktu penggandaan, pembenihan.ABSTRACKNannochloropsis sp. is a microalgae that is cultivated as natural food and has an important role in the effort of hatching fish, shrimp, crabs and other marine biota. This study aims to determine the effect of urea concentration on the growth of Nannochloropsis sp cell population and the best concentration of fertilizers for the growth of Nannochloropsis sp. Cell populations. The method used in this study is the experimental method using a Completely Randomized Design (CRD), with 6 treatments namely giving KW21 fertilizer as much as 1 ml / L as a control, 0 mg / L, 15 mg / L, 30 mg / L, 45 mg / L and 60 mg / L were repeated 4 times. Observation of density is carried out for 24 hours. The parameters observed included population density of Nannochlorpsis sp. Cells, relative growth, self doubling time and specific growth. The results showed that the highest density of cell populations was Nannochlorpsis sp. obtained at the concentration of urea fertilizer 45 mg / L with a cell population density of 2.2625 million cells / mL which was reached at 24 hours after the adaptation phase. And the highest relative growth was 50.83% from the initial population density, and the shortest self doubling time was 40.8 hours with a specific growth of 0.8625% per hour. Based on the results of this study, it is recommended to cultivate Nannochloropsis sp. to increase the growth of cell populations is urea with a concentration of 45 mg / L.Keywords: natural feed, cultivation, density, doubling time, seeding.

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Journal Info

Abbrev

JK

Publisher

Subject

Description

This journal encompasses original research articles, review articles, and short communications, including: Marine and fisheries ecology and biology, Marine fisheries, Marine technology, biotechnology, Mariculture, Marine processes and dynamics, Marine conservation, Marine pollution, Marine and ...