The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment

Sholeh Avivi (Department of Agroecotechnology, Faculty of Agriculture, Jember University)
Ralf G. Dietzgen (Queensland Agricultural Biotechnology Centre (QABC) Australia)
Colleen M. Higgins (Queensland Agricultural Biotechnology Centre (QABC) Australia)
Sudarsono . (Department of Agronomy, Faculty of Agriculture, Bogor Agriculture University)

UNEJ e-Proceeding
Indonesian Protein Society (IPS), International Seminar and Workshop 2014

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.