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The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Avivi, Sholeh; Dietzgen, Ralf G.; Higgins, Colleen M.; ., Sudarsono
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
Pengaruh Ukuran Embriozigot terhadap Regenerasi Beberapa Klon Kakao Avivi, Sholeh; Restanto, Didik Pudji; Widyastuti, Tri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.245 KB) | DOI: 10.31258/jnat.13.3.237-243

Abstract

This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.
Morphological Diversity and Molecular RAPD Markers of Sugarcane Mutane (Saccharum officinarum L.) in Inundation Tolerance Avivi, Sholeh; Suliswanto, Eko Nur; Restanto, Didik Pudji; Miswar, Miswar; Syamsunihar, Anang; Soeparjono, Sigit; Hartatik, Sri
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1304

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The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7 - 56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
Pengembangan Budidaya Sayur Organik di Pesantren Nurul Islam Jember dengan Teknologi Komposting Takakura dan Sistem Tanam Vertikultur Mohammad Nur Khozin; Dwi Erwin Kusbianto; Sholeh Avivi; Bakhroini Habriantono; Riza Yuli Rusdiana
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 4 (2021)
Publisher : Universitas Mataram

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Nurul Islam Islamic boarding school Jember is one of the modern Islamic boarding schools that teaches religion, science, and technology. Organic vegetable cultivation training using Takakura technology and a verticulture planting system aims to increase the knowledge awareness, and skills of the students. Besides, this activity has a positive impact on health, waste management, environmental harmony, vegetable crop production, land effectiveness and adds economic value. Takakura technology produces odorless and nutritious compost products. Takakura compost is used as a growing medium in verticulture pipes. The skills of cultivating organic vegetables using Takakura technology and a verticulture planting system are very useful for the students. Technically, the implementation of this program includes material presentation, leaflet giving, and hands-on practice. Cultivation that is carried out includes making media, preparing seeds, preparing planting media, transplanting seeds into verticulture media, and plant care. The result of training activity can improve the knowledge and competence of student to produce organic fertilizer and vegetable through the technology that transfer for them.
Efek Aplikasi Synechococcus sp. pada Daun dan Pupuk NPK terhadap Parameter Agronomis Kedelai R. Soedradjad; Sholeh Avivi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 3 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.324 KB) | DOI: 10.24831/jai.v33i3.1260

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Synechococcus sp. is a species photosynthetic bacterium that has symbiotic mutualism with plant.  Research on this field is not many. Foliar application of this bacterium may increase the growth and yield characteristics.  The aim of this research was to determine the effect of Synechococcus sp. application and NPK fertilizer on soybean growth and yield.  The research was conducted in Pusat Inkubator Agribisnis (PIA) Jember University on February until May 2004.  Split plot design was used with 2 factors, Synechococcus sp. as sub plot (B0: without bacteria and B1: with bacteria application) and NPK fertilizers as main plot (P0: 0 g/plant; P1: 0.347 g/plant; and P2: 0.875 g/plant) with three replications. The result showed that the interaction between Synechococcus sp. and NPK fertilizers treatments was not significant.  The  bacteria applications significantly increasing plants growth (42.9%), leaf area index (294.6%), number of productive stem per plant (141.3%), number of productive nodes per plant (40.3%), pods weight per plant (175.2%), number of pods per plant (152.8%), grain weight per plant (80.5%), dry weight (209.8%), and 100 grains weight per plant (3.4%).  The fertilizers significantly affected only on plants growth (44.6%) and number of pods per plant (29.4 %).    Key words:  Glycine, Synechococcus sp., NPK  
EFISIENSI SERAPAN UNSUR 15N-UREA DAN PROPORSI FIKSASI N OLEH TANAMAN KEDELAI TIDAK BERKOTILEDON PADA BUDIDAYA JENUH AIR Sholeh Avivi; Wahju Q. Mugnisjah; Komarudin Idris; Elsye L. Sisworo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 25 No. 1 (1997): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1282.858 KB) | DOI: 10.24831/jai.v25i1.1654

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This pot experiment was to evaluate the influences of cotyledons detachment at seedling stage on the efficiency of labelled N-urea uptake and proportion of N-fixation by soybean cv. Wilis grown under saturated soil culture. Based on the result of preliminary experiment, cotyledons detachment was held at 7 days after sowing (das). The cotyledons detachment significantly reduced N-urea uptake efficiency and N-fixed proportion. The N-urea uptake efficiency at 21 days, R4, R5, R6, and R8 stages by cotyledons-detached plant were 30.5 %, 24.6 %, 23.7 %, 24.0 %, and 22.45 of the total N assimilated. Those of the cotyledons undetached-plant (control plant), the value were 31.6 %, 24.05, 24.7 %, 2.7 %, and 23.6 % respectively. At R8 stage, the cotyledons-detached plant has the amount of N-fixed proportion of 54.2 and N-soil of 23.4 %, whereas those of the control one had the amount of 49.7 and 26.7 % respectively. N-urea uptake, N-fixed, and N-soil uptake by the cotyledons- detached plant were lower than that of the control one (i.e. 69, 80, and 645 of the control respectively). The cotyledons detachment also result in inferior vegetative and productive growth of the plant in terms of decreased root dry weight (21.1 %), leave dry weight (18.8 %), plant dry weight (26.7 %), pot dry weight (23.8 %), grain number/pot (32.8 %), and grain dry weight (26.9 %). It seems that the lower growth and yield of the cotyledons-detached soybean paint were caused by the lower total N-uptake due to cotyledons detachment.
Regenerasi Embriogenesis Somatik pada Beberapa Klon Kakao Indonesia dari Eksplan Bunga Sholeh Avivi; Adi Prawoto; Reny Fauziah Oetami
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.211 KB) | DOI: 10.24831/jai.v38i2.1798

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<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This research was aimed to observe the response of different clones and specifi c organs due to the somatic embryogenesis regeneration. It was arranged in factorial randomized completely design with three replication. The fi rst factor was cocoa clones i.e. ICCRI 01, ICCRI 02, ICCRI 03, ICCRI 04, KW 514, RCC72, and Sca 6. The second factor was fl ower parts i.e. petal, staminode and anther. Every explant was regenerated on initiation, induction, multiplication and rooting media. Almost all treatments showed high response of embryogenic calli which range 89.5 to 100% at initial stage, but different results were found at the following process of somatic embryogenesis. The experiment showed that each clones and each different part of fl ower had different response to somatic embryogenesis. The highest response of the explant number resulted from Sca 6 clone, which produce 35.8% embryo with average number of embryo per explant (1.34) followed by RCC 72 (28.4%, averaged 0.7) ICCRI 03 (24.7%, averaged 1.3) ICCRI 04 (18.6%, averaged 0.6). While ICCRI 02 showed the lowest responsive clone. Especially for ICCRI 01, 55.8% explant was rooted and only 1.3% explant producing embryo. The highest response of somatic embryo was resulted form petal. Keywords: somatic embryogenesis, fl ower explant, Cocoa
Toleransi Berbagai Varietas Tebu terhadap Penggenangan pada Fase Bibit Berdasarkan Karakter Morfologi dan Anatomi Sholeh Avivi; Anang Syamsunihar; Sigit Soeparjono; dan Muhammad Chozin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.239 KB) | DOI: 10.24831/jai.v46i1.14081

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Identification of Indonesia sugarcane varieties tolerant to waterlogging has not been done extensively. Information on varieties tolerant to waterlogging is required for seedling establishment in waterlogged areas. The purpose of this research was to identify the sugarcane varieties responses to several duration levels of waterlogging at seedling stage. The planting materials used were collection of Sugar Factory Semboro including VMC 76-16, BL, PS 862, PS 864, and PS 881. This research was arranged in randomized complete block design (RCBD) with two factors and three replications. The first factor was 5 varieties; the second factor was waterlogging treatment with four levels, i.e. without waterlogging, 2, 4, and 6 weeks of waterlogging in the bucket. The results showed different level of tolerance to water logging among the varities. PS 881 and VMC 76-16 varieties were the most tolerant to waterlogging supported by its ability to maintain plant height, root volume, root and shoot dry weight after been waterlogged for 6 weeks. These varieties were also able to establish aerenchyme tissue and increased the stomatal density. PS 862 was the least tolerant variety to waterlogging. Keywords: aerenchyme, morphology, stomatal density, waterlogging, water stress
Multiplikasi Tunas dan Aklimatisasi Tiga Varietas Pisang: Raja Nangka, Kepok, dan Mas Sholeh Avivi; Soetilah Hardjo Soedarmo; Priyanto Andi Prasetyo
Jurnal Hortikultura Indonesia Vol. 4 No. 2 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.657 KB) | DOI: 10.29244/jhi.4.2.83-89

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ABSTRACTRaja  Nangka,  Kepok,  and  Mas  are  local  popular  cultivar  of  banana  in Indonesia.  Mass propagation is needed for extended area of planting. The aims of this research was to determine the optimum  concentration  of  plant  growth regulator  for  planlet  regeneration.  The  research  was conducted at Department of Agronomy, Faculty of Agriculture, University of Jember.  We used three stages regeneration process: multiplication, rooting, and acclimatization. The multiplication stage was arranged in factorial experiment in a  completely  randomized  design with five replications. The first factor was kinetin concentration (3.5  ppm, 4.0  ppm, 4.5 ppm, 5.0 ppm, 5.5  ppm,  and 6.0 ppm) and the second factor was kind of banana (Raja Nangka, Kepok, and Mas). The rooting stage was also  arranged in factorial   completely  randomized design with five replication s  with the first factor was NAAconcentration  (0, 0.5, 1.0, and 1.5 ppm)  and the second factor was kind of banana. The result showedthat the best kinetin concentration on the multipliction stage was 5.0 ppm (the number of shoot 2.6). Raja  Nangka variety gave the highest number of shoot (2.7 shoot per  explant). The best concentration  atrooting stage was 0.5 ppm NAA which gave the number of root 7.5 and 3.2 cm  in  length. Most of planlet (90%) could be acclimatized on acclimatization media.Key words: acclimatization, kinetin, multiplicatin, NAAABSTRAKPisang Raja Nangka,  Kepok, dan  Mas  merupakan jenis pisang lokal yang terdapat di Indonesia dan digemari mas yarakat. Penelitian ini bertujuan untuk mengetahui konsentrasi zat pengatur tumbuh yang sesuai untuk regenerasi hingga menghasilkan planlet. Penelitian dilaksanakan di Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Jember. Penelitian terdiri ata s 3 tahap yaitu tahap m ultiplikasi tunas, tahap per akaran dan tahap aklimatisasi. Tahap multiplikasi  dilakukan  dengan  Rancangan  Acak  Lengkap faktorial  dengan  2  faktor.   Faktor  pertama  adalah konsentrasi  kinetin (3. 5 ppm ,   4. 0   ppm ,  4.5   ppm , 5.0   ppm , 5. 5   ppm ,  dan 6. 0 ppm) dan faktor kedua adalah jenis pisang ( Raja Nangka, Kepok, dan Mas) . Pada  tahap perakaran tunas hasil multiplikasi digunakan  Rancangan Acak Lengkap faktorial dengan dua  faktor. Faktor pertama adalah konsentrasi  NAA (0  ppm , 0.5 ppm , 1.0  ppm , dan  1. 5 ppm) ,   s edangkan  faktor  kedua  adalah  jenis  pisang dengan  taraf  yang  sama.  Ulangan dilakukan 5 kali. Uji lanjut dilakukan dengan uji Duncan 5%. Hasil penelitian menunjukkan bahwa konsentrasi kinetin terbaik diperoleh pada perlakuan  5. 0   ppm   (rata - rata  ju mlah  tunas 2.6). Jenis  pisang  Raja  Nangka  memberikan  rata- rata  ju mlah  tunas tertinggi sebanyak 2. 7 tunas  per - eksplan. Pada tahap perakaran, panjang akar opt imal diperoleh pada perlakuan 0. 5 ppm  NAA,  dengan  rata - rata  panjang akar  3. 2  cm  dengan  jumlah  akar  rata - rata  sebanyak   7. 5   buah . Tingkat keberhasilan aklimatisasi mencapai hingga 90%.Kata kunci: aklimatisasi, kinetin, multiplikasi, NAA
Response of Morphological and Physiological Characteristics of 4 Flood- tolerant Sugarcane (Saccharum Officinarum L.) Cultivars to ZA (Zwavelzuur Ammoniac) fertilizer application in Jember and Bondowoso Sholeh Avivi; Cacuk Purnomo; Ayu Puspita Arum; Sugeng Winarso; Sri Hartatik
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2873.457 KB) | DOI: 10.22146/ipas.40173

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The business expansion of sugarcane can be done using marginal land, such as wet land. This study attempted to get the right doses of ZA fertilizer applied to several sugarcane cultivars, which are tolerant to flooding in two different locations. The research was carried out in Jubung Agrotechnopark garden,  University of Jember and seed gardens of Dewisri Bondowoso, from November 2015 to November 2016. The experiment was arranged in factorial randomized complete block design. The first factor was the doses of ZA fertilizer, consisting of 3 levels of treatment, i.e. 0 kg.ha-1 (without ZA fertilizer), 500 kg.ha-1 and 1000 kg.ha-1. The second factor was 4 sugarcane cultivars,  consisting of flood-tolerant sugarcane cultivars from 2014 screening result, i.e. PSJT 941, Bulu Lawang, PS 865 and Kidang Kencana. The results showed that the application of ZA fertilizer at 1000 kg.ha-1 affected the height of stems, the number of tillers, diameter of the stem and the number of segments. Meanwhile, the application of ZA fertilizer at 500 kg.ha-1 affected the number of leaves, fresh weight of shoots, dry weight of shoots, fresh weight of roots, dry weight of roots, brix content, and the content of sucrose and reducing sugar. Flood-tolerant sugarcane cultivars are shown by the highest brix and sucrose fertilizer, observed in  PS 865, Bulu Lawang, PSJT 941, and Kidang Kencana, sequentially. The highest content of reducing sugar was found in PSJT 941, PS 865, Kidang Kencana, and Bulu Lawang, successively.
Co-Authors - Asmuni Abdul jalil Abdul Jalil Adam Ramadhan Adhitya Wardhono Adi Prawoto Akbar, Muhammad Mizan Ilham Alvan Maulana Azzini Anang Syamsunihar Andri Wahyudi Anggira, Desi Aprila Iga Mufidah Arum, Ayu Puspita Arya Wiranegara Aurelya, Lalyta Azmi Saleh Azzarah Bakhroini Habriantono Bambang Kusmanadhi Bambang Sugiharto Budi Kristanto Cacuk Purnomo Cahyani, Ardhya Pramisti Regita Choirunnisa, Eviyanti Colleen M. Higgins Colleen M. Higgins, Colleen M. dan Muhammad Chozin Dewi Kusuma Wardani Djoko Soejono Dwi Erwin Kusbianto Dyah Ayu Savitri Elsye L. Sisworo Faesol, Nurul Galang Rizki Ramadhan Gatot Subroto HAJRIAL ASWIDINNOOR Haliza, Nurhayadatul Halwiyah, Lailatul Hartita, Margaretha Putri Hoesain, Mohammad Ikrarwati Ikrarwati Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Irfa' Yudayantho Kacung Hariyono Ketut Anom Wijaya Kim, Kyung Min KOMARUDIN IDRIS Laksono Trisnantoro Lenny Widjayanthi Maulidina, Niswah Saffanah Moh. Syahrul Munir Mohammad Nur Khozin Mohammad Ubaidillah Muhammad Ghufron Rosyady Rosyady Muhammad Hazmi Nasir, M Abd Nisa Budi Arifiana Novelia, Eka Putri Parawita Dewanti Pio Tifar Ananda Prabowo, Rachmad Udhi Pradika, Yoga Reno Ayom Prayoga, Mohammad Candra Priyanto Andi Prasetyo Puji Rahayu Puspito, Agung Nugroho Qori’ah, Ciplis Gema Raden Soedradjad Rahmawati Rahmawati Ralf G. Dietzgen Ralf G. Dietzgen, Ralf G. Ratih Apri Utami Reny Fauziah Oetami Restanto, Didik Restanto, Didik Pudji Rusdiana, Riza Yuli Satriyas Ilyas Setiyono Setiyono Setiyono Setiyono Sigit Soeparjono Sitompul, Novrida Yanti Slameto Slameto Slameto Slameto Sobah, Naqiyyah Nada Soetilah Hardjo Soedarmo Sri Hartatik Subroto, Gatot Sudarsono Sudarsono . Sudarsono Sudarsono Sugeng Winarso Sugeng, Santoso Suliswanto, Eko Nur Suud, Hasbi Mubarak Tri Agus Siswoyo TRI HANDOYO Tri Widyastuti Ubaidillah, Mohammad Viki Bayu Wibisono Wahju Q. Mugnisjah Wahyu Indra Duwi Fanata Wulanjari, Distiana Yulianti, Annisa Zahrosa, Dimas Bastara Zakariyya, Fakhrusy Zulfa Maulida