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The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Avivi, Sholeh; Dietzgen, Ralf G.; Higgins, Colleen M.; ., Sudarsono
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
Pengaruh Ukuran Embriozigot terhadap Regenerasi Beberapa Klon Kakao Avivi, Sholeh; Restanto, Didik Pudji; Widyastuti, Tri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.245 KB) | DOI: 10.31258/jnat.13.3.237-243

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This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.
Morphological Diversity and Molecular RAPD Markers of Sugarcane Mutane (Saccharum officinarum L.) in Inundation Tolerance Avivi, Sholeh; Suliswanto, Eko Nur; Restanto, Didik Pudji; Miswar, Miswar; Syamsunihar, Anang; Soeparjono, Sigit; Hartatik, Sri
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1304

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The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7 - 56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
Pengembangan Budidaya Sayur Organik di Pesantren Nurul Islam Jember dengan Teknologi Komposting Takakura dan Sistem Tanam Vertikultur Mohammad Nur Khozin; Dwi Erwin Kusbianto; Sholeh Avivi; Bakhroini Habriantono; Riza Yuli Rusdiana
Jurnal Pengabdian Magister Pendidikan IPA Vol 4 No 4 (2021)
Publisher : Universitas Mataram

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Nurul Islam Islamic boarding school Jember is one of the modern Islamic boarding schools that teaches religion, science, and technology. Organic vegetable cultivation training using Takakura technology and a verticulture planting system aims to increase the knowledge awareness, and skills of the students. Besides, this activity has a positive impact on health, waste management, environmental harmony, vegetable crop production, land effectiveness and adds economic value. Takakura technology produces odorless and nutritious compost products. Takakura compost is used as a growing medium in verticulture pipes. The skills of cultivating organic vegetables using Takakura technology and a verticulture planting system are very useful for the students. Technically, the implementation of this program includes material presentation, leaflet giving, and hands-on practice. Cultivation that is carried out includes making media, preparing seeds, preparing planting media, transplanting seeds into verticulture media, and plant care. The result of training activity can improve the knowledge and competence of student to produce organic fertilizer and vegetable through the technology that transfer for them.
Multiplikasi Tunas dan Aklimatisasi Tiga Varietas Pisang: Raja Nangka, Kepok, dan Mas Sholeh Avivi; Soetilah Hardjo Soedarmo; Priyanto Andi Prasetyo
Jurnal Hortikultura Indonesia Vol. 4 No. 2 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.657 KB) | DOI: 10.29244/jhi.4.2.83-89

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ABSTRACTRaja  Nangka,  Kepok,  and  Mas  are  local  popular  cultivar  of  banana  in Indonesia.  Mass propagation is needed for extended area of planting. The aims of this research was to determine the optimum  concentration  of  plant  growth regulator  for  planlet  regeneration.  The  research  was conducted at Department of Agronomy, Faculty of Agriculture, University of Jember.  We used three stages regeneration process: multiplication, rooting, and acclimatization. The multiplication stage was arranged in factorial experiment in a  completely  randomized  design with five replications. The first factor was kinetin concentration (3.5  ppm, 4.0  ppm, 4.5 ppm, 5.0 ppm, 5.5  ppm,  and 6.0 ppm) and the second factor was kind of banana (Raja Nangka, Kepok, and Mas). The rooting stage was also  arranged in factorial   completely  randomized design with five replication s  with the first factor was NAAconcentration  (0, 0.5, 1.0, and 1.5 ppm)  and the second factor was kind of banana. The result showedthat the best kinetin concentration on the multipliction stage was 5.0 ppm (the number of shoot 2.6). Raja  Nangka variety gave the highest number of shoot (2.7 shoot per  explant). The best concentration  atrooting stage was 0.5 ppm NAA which gave the number of root 7.5 and 3.2 cm  in  length. Most of planlet (90%) could be acclimatized on acclimatization media.Key words: acclimatization, kinetin, multiplicatin, NAAABSTRAKPisang Raja Nangka,  Kepok, dan  Mas  merupakan jenis pisang lokal yang terdapat di Indonesia dan digemari mas yarakat. Penelitian ini bertujuan untuk mengetahui konsentrasi zat pengatur tumbuh yang sesuai untuk regenerasi hingga menghasilkan planlet. Penelitian dilaksanakan di Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Jember. Penelitian terdiri ata s 3 tahap yaitu tahap m ultiplikasi tunas, tahap per akaran dan tahap aklimatisasi. Tahap multiplikasi  dilakukan  dengan  Rancangan  Acak  Lengkap faktorial  dengan  2  faktor.   Faktor  pertama  adalah konsentrasi  kinetin (3. 5 ppm ,   4. 0   ppm ,  4.5   ppm , 5.0   ppm , 5. 5   ppm ,  dan 6. 0 ppm) dan faktor kedua adalah jenis pisang ( Raja Nangka, Kepok, dan Mas) . Pada  tahap perakaran tunas hasil multiplikasi digunakan  Rancangan Acak Lengkap faktorial dengan dua  faktor. Faktor pertama adalah konsentrasi  NAA (0  ppm , 0.5 ppm , 1.0  ppm , dan  1. 5 ppm) ,   s edangkan  faktor  kedua  adalah  jenis  pisang dengan  taraf  yang  sama.  Ulangan dilakukan 5 kali. Uji lanjut dilakukan dengan uji Duncan 5%. Hasil penelitian menunjukkan bahwa konsentrasi kinetin terbaik diperoleh pada perlakuan  5. 0   ppm   (rata - rata  ju mlah  tunas 2.6). Jenis  pisang  Raja  Nangka  memberikan  rata- rata  ju mlah  tunas tertinggi sebanyak 2. 7 tunas  per - eksplan. Pada tahap perakaran, panjang akar opt imal diperoleh pada perlakuan 0. 5 ppm  NAA,  dengan  rata - rata  panjang akar  3. 2  cm  dengan  jumlah  akar  rata - rata  sebanyak   7. 5   buah . Tingkat keberhasilan aklimatisasi mencapai hingga 90%.Kata kunci: aklimatisasi, kinetin, multiplikasi, NAA
Response of Morphological and Physiological Characteristics of 4 Flood- tolerant Sugarcane (Saccharum Officinarum L.) Cultivars to ZA (Zwavelzuur Ammoniac) fertilizer application in Jember and Bondowoso Sholeh Avivi; Cacuk Purnomo; Ayu Puspita Arum; Sugeng Winarso; Sri Hartatik
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2873.457 KB) | DOI: 10.22146/ipas.40173

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The business expansion of sugarcane can be done using marginal land, such as wet land. This study attempted to get the right doses of ZA fertilizer applied to several sugarcane cultivars, which are tolerant to flooding in two different locations. The research was carried out in Jubung Agrotechnopark garden,  University of Jember and seed gardens of Dewisri Bondowoso, from November 2015 to November 2016. The experiment was arranged in factorial randomized complete block design. The first factor was the doses of ZA fertilizer, consisting of 3 levels of treatment, i.e. 0 kg.ha-1 (without ZA fertilizer), 500 kg.ha-1 and 1000 kg.ha-1. The second factor was 4 sugarcane cultivars,  consisting of flood-tolerant sugarcane cultivars from 2014 screening result, i.e. PSJT 941, Bulu Lawang, PS 865 and Kidang Kencana. The results showed that the application of ZA fertilizer at 1000 kg.ha-1 affected the height of stems, the number of tillers, diameter of the stem and the number of segments. Meanwhile, the application of ZA fertilizer at 500 kg.ha-1 affected the number of leaves, fresh weight of shoots, dry weight of shoots, fresh weight of roots, dry weight of roots, brix content, and the content of sucrose and reducing sugar. Flood-tolerant sugarcane cultivars are shown by the highest brix and sucrose fertilizer, observed in  PS 865, Bulu Lawang, PSJT 941, and Kidang Kencana, sequentially. The highest content of reducing sugar was found in PSJT 941, PS 865, Kidang Kencana, and Bulu Lawang, successively.
Correlation Between Secondary Metabolites of Leaf and the Resistance to Leaf Rust (Hemileia vastatrix) on Several Arabica Coffee Clones Gatot Subroto; Dwi Erwin Kusbianto; Sholeh Avivi; Slameto Slameto; Setiyono Setiyono
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.61 KB) | DOI: 10.22146/ipas.42124

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Indicator of coffee resistance to leaf rust attack (Hemileia vastatrix) is needed to select superior coffee plants resistant to biotic stress. This study aims to find the relationship between the content of secondary metabolites and the intensity of leaf rust attack, so that it becomes a reference in the selection of future coffee plants. The experimental design used was a completely randomized design (CRD) with several coffee clones as a treatment. This test tested 5 Arabica coffee clones consisting of Komasti, Maragogik, Usda, Andong sari, and HDT clones. Each consists of 3 replications, and each replication consists of 2 sample plants. Observations were made by observing the intensity of the attack, and plant metabolites such as phenolic content, flavonoids and antioxidant activity were observed when the leaves had been attacked by Hemileia vastatrix. Correlation of leaf rust attack levels with phenolic content, flavonoids and antioxidant activity showed a relationship between each observation variable. Flavonoid content in certain conditions can be used as an indicator to get Arabica coffee plants that are resistant to the attack of leaf rust.
Mikropropogasi Pisang Abaca (Musa textlilis Nee) Melalui Teknik Kultur Jaringan Sholeh Avivi; Ikrarwati Ikrarwati
Jurnal Ilmu Pertanian Vol 11, No 2 (2004): Desember
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.59949

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Pertumbuhan Sawi yang Berasosiasi dengan Bakteri Synechococcus sp. pada Berbagai Kondisi Media Salinitas - Asmuni; Sholeh Avivi; Sugeng Winarso
Agrovigor Vol 10, No 1 (2017): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.915 KB) | DOI: 10.21107/agrovigor.v10i1.2844

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Sawi hijau merupakan salah satu jenis sayur yang di konsumsi oleh masyarakat Indonesia yang memiliki banyak manfaat diantaranya untuk mencegah kanker, hipertensi, penyakit jantung, membantu kesehatan sistem pencernaan, mencegah dan mengobati penyakit pellagra, serta menghindarkan ibu hamil dari anemia. Tanaman sawi pada umumnya dapat hidup di dataran rendah maupun dataran tinggi, akan tetapi lebih baik penanamannya dilakukan di dataran tinggi. Penelitian tentang resistensi sawi di media salin belum banyak dilakukan. Tujuan dari penelitian ini adalah untuk mengetahui respon tanaman sawi hijau yang telah berasosiasi dengan bakteri Synechococcus spterhadap berbagai kondisi salinitas. Penelitian ini dilakukan mulai bulan November 2016 sampai Januari 2017 di Desa Bendoarum Kecamatan Wonosari Kabupaten Bondowoso. Adapun bahan tanam yang digunakan adalah sawi, biakan bakteri fotosintetik (Synechococus sp), air, tanah, pasir, garam NaCl, pupuk (urea, SP-36 dan KCl). Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 4 kali ulangan. Perlakuan salinitas diberikan berdasarkan penambahan konsentrasi garam (NaCl) yaitu kontrol (0 ppm), 2500 ppm, 5000 ppm, 7500 ppm, dan 10.000 ppm. Respon ta tanaman terhadap perlakuan didasarkan pada variabel jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Data tersebut dianalisis menggunakan uji Anova dan Jarak Berganda Duncan (DMRT) dengan signifikansi pada taraf 5%. Respon tanaman sawi terhadap penambahan garam menunjukkan hasil yang berbeda nyata pada semua parameter pertumbuhan yang meliputi jumlah daun, jumlah klorofil, panjang akar, volume akar, berat basah, dan berat kering. Respon tanaman yang tercekam garam baik yang diberikan bakteri Synechococcus sp atau yang tidak diberikan menunjukkan hasil menurun dan berbeda nyata pada parameter panjang akar, berat basah, dan berat kering. Pemberian bakteri Synechococcus sp pada tanaman yang ditanam dilahan salin tidak berpengaruh nyata pada parameter pertumbuhan tanaman, tapi cenderung meningkatkan berat basah tanaman, berat kering tanaman, dan panjang akar tanaman.Kata kunci : Salinitas, Sawi Hijau, Synechococcus sp, pertumbuhan tanaman
Pengaruh cekaman genangan terhadap pertumbuhan dan hasil beberapa varietas tomat (Lycopersicum esculentum Mill.) Sholeh Avivi; Aprila Iga Mufidah; Tri Agus Siswoyo; Didik Pudji Restanto
Agrovigor Vol 15, No 1 (2022): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v15i1.8866

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Usaha untuk meningkatkan produksi tomat pada lahan tercekam genangan dapat dilakukan dengan menguji varietas-varietas tomat yang sudah beredar terhadap cekaman genangan. Uji dilakukan dengan mengamati parameter pertumbuhan dan hasil beberapa varietas tomat. Penelitian ini bertujuan untuk mengetahui toleransi beberapa varietas tomat terhadap cekaman genangan. Metode yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) faktorial dengan 3 kali ulangan. Varietas tanaman tomat merupakan faktor pertama dengan 5 taraf yaitu a) V1 Sakina F1, b) V2 Tora, c) V3 Rewako F1, dan d) V4 Mawar. Sedangkan faktor yang kedua adalah cekaman genangan dengan 3 taraf, yaitu a) T1, perlakuan kondisi cekaman genangan 5 cm, b) T2, perlakuan kondisi cekaman genangan 10 cm, c) T3, perlakuan kondisi cekaman genangan 15 cm. Hasil penelitian menunjukkan bahwa varietas Rewako mempunyai karakter lebih toleran terhadap genangan yang ditunjukkan pada parameter rerata tinggi tanaman, ukuran diameter buah, berat buah dan berat tajuk atas tanaman. 
Co-Authors - Asmuni Abdul Jalil Abdul jalil Adam Ramadhan Adhitya Wardhono Adi Prawoto Akbar, Muhammad Mizan Ilham Alvan Maulana Azzini Anang Syamsunihar Andri Wahyudi Anggira, Desi Aprila Iga Mufidah Arum, Ayu Puspita Arya Wiranegara Aurelya, Lalyta Azmi Saleh Azzarah Bakhroini Habriantono Bambang Kusmanadhi Bambang Sugiharto Budi Kristanto Cacuk Purnomo Cahyani, Ardhya Pramisti Regita Choirunnisa, Eviyanti Chozin, dan Muhammad Colleen M. Higgins Colleen M. Higgins, Colleen M. Dewi Kusuma Wardani Djoko Soejono Dwi Erwin Kusbianto Dyah Ayu Savitri Elsye L. Sisworo Faesol, Nurul Galang Rizki Ramadhan Gatot Subroto HAJRIAL ASWIDINNOOR Haliza, Nurhayadatul Halwiyah, Lailatul Hartita, Margaretha Putri Hoesain, Mohammad Ikrarwati Ikrarwati Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Irfa' Yudayantho Kacung Hariyono Ketut Anom Wijaya Kim, Kyung Min KOMARUDIN IDRIS Laksono Trisnantoro Lenny Widjayanthi Maulida, Zulfa Maulidina, Niswah Saffanah Moh. Syahrul Munir Mohammad Nur Khozin Mohammad Ubaidillah Muhammad Ghufron Rosyady Rosyady Muhammad Hazmi Nasir, M Abd Nisa Budi Arifiana Novelia, Eka Putri Parawita Dewanti Pio Tifar Ananda Prabowo, Rachmad Udhi Pradika, Yoga Reno Ayom Prayoga, Mohammad Candra Priyanto Andi Prasetyo Puji Rahayu Puspito, Agung Nugroho Qori’ah, Ciplis Gema Raden Soedradjad Rahmawati Rahmawati Ralf G. Dietzgen Ralf G. Dietzgen, Ralf G. Ratih Apri Utami Reny Fauziah Oetami Restanto, Didik Restanto, Didik Pudji Rifngatul ‘Atiqoh Rusdiana, Riza Yuli Satriyas Ilyas setiyono Setiyono Setiyono Setiyono Setiyono Sigit Soeparjono Sitompul, Novrida Yanti Slameto Slameto Slameto Slameto Sobah, Naqiyyah Nada Soetilah Hardjo Soedarmo Sri Hartatik Sri Hartatik Subroto, Gatot Sudarsono Sudarsono . Sudarsono Sudarsono Sugeng Winarso Sugeng, Santoso Suliswanto, Eko Nur Suud, Hasbi Mubarak Tri Agus Siswoyo TRI HANDOYO Tri Widyastuti Ubaidillah, Mohammad Wahju Q. Mugnisjah Wahyu Indra Duwi Fanata Wibisono, Viki Bayu Wulanjari, Distiana Yulianti, Annisa Zahrosa, Dimas Bastara Zakariyya, Fakhrusy