Weight loss due stres during transportation had become economic defect on livestock business. According to this situation, an operational research using 136 cattle were held to evaluated effect of vitamin C treatment since the capability of vitamin C to reduced stres. There are 68 heads which is oral treated using 500 mg vitamin C and 68 control, respectively. Observation were done on May-August 2014, during cattle transportation from Karang Endah Sub district, Lampung Tengah Municipal, Lampung Province to Palembang, Sumatera Selatan Province. Transportation repeated 6 times using trucks consist of half treated, which has odd tag, and control cattle. Cattle weighing were done before and after transportation, while data analyzed using t-test. Observation showed that the weight lost difference between treated (10,15%) and control (9,74%) is 0,41% (P<0,05). Result also showed that vitamin C could saving of Rp.647.595,00/truck/ transportation.
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