International Journal of Biosciences and Biotechnology
Vol 4 No 2 (2017)

ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE

I Ketut Suada (Department of Agroecotechnology, Faculty of Agriculture, Udayana University, Bali)
Anak Agung Ngurah Gede Suwastika (Department of Agroecotechnology, Faculty of Agriculture, Udayana University, Bali)



Article Info

Publish Date
02 Apr 2017

Abstract

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.

Copyrights © 2017






Journal Info

Abbrev

jbb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Environmental Science

Description

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of ...