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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)
Anak Agung Ngurah Gede Suwastika
Department Of Agroecotechnology, Faculty Of Agriculture, Udayana University, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE I Ketut Suada; Anak Agung Ngurah Gede Suwastika
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.026 KB) | DOI: 10.24843/IJBB.2017.v04.i02.p01

Abstract

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.
APPLICATION OF Trichoderma spp. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (Plasmodiophora brassicae WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE I Ketut Suada; Anak Agung Ngurah Gede Suwastika; I Kadek Ngestika Pradnyana; Nataliya Shchegolkova; Rodion Poloskin; Oleg Gladkov; Olga Yakimenko; Aleksey Stepanov
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.407 KB) | DOI: 10.24843/IJBB.2019.v06.i02.p01

Abstract

The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%. This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.
Kajian Pemanfaatan Limbah Sabut Kelapa Menjadi Larutan Mikroorganisme Lokal PUTU ANANTA WIDHIA DHARMA; ANAK AGUNG NGURAH GEDE SUWASTIKA; NI WAYAN SRI SUTARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Study of Coconut Husk Waste Utilization Become Local Microorganism Fermented Solution The coconut husk has the most component in the coconut fruit, it cover 35 % of their composition. The research aim to understand the impact of the substrate dose, fermentation period and interaction between factors in quality measurement of local microorganism solution. This research use randomized block design and factorial treatment arrangement. Parameter measurement in research were C organic, total N, C/N ratio, available P and K, C/P ratio, pH, salinity, total bacterial population and total fungi population. The result of the research show that significant interaction between factor with exception on total N. Overall dose of substrate show that the 300 grams and three weeks fermentation has the best result.
Analisis Kualitas Larutan Mol (Mikroorganisme Lokal) Berbasis Ampas Tahu NI WAYAN MARSININGSIH; A A NGURAH GEDE SUWASTIKA; NI WAYAN SRI SUTARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT   Analysis of Local Mikroorganisms Solution Quality Based on Tofu dregs. The experiment was conducted at the Soil Laboratory, Faculty of Agriculture, Udayana University in January until March 2014. The purpose of this research was to determine the effects of concentration and length of fermentation tofu dregs to the quality of local microorganisms solution. The design of this research used a randomized block design factorial pattern. The first factor was the concentration of tofu dregs consisted of 0 g; 300 g; and 600 g. The second factor was the length of fermentation, consisted of one, three, and five weeks. The observed parameters are: the nature of biological, physical and chemical solution of local microorganisms. Physical characteristics include color and odor, biological properties covering a total population of bacteria and fungi. Chemical properties include pH, total-N content, and available-P. The result of this research showed that treatment of 600 g tofu dregs and five weeks length of fermentation provides the best results. It can be seen from the total population  bacteria  (29,80 x 108 cfu mL-1), total-N (0,06 %) and available-P (199,38 mg kg-1).   Keywords: tofu dregs, concentration, length of fermentation, local microorganisms
Pengaruh Komposisi Media Tanam dan Pemberian Pupuk Anorganik Majemuk Terhadap Pertumbuhan Awal Bibit Kelapa Sawit (Elaeis guineensis Jacq.) ARJUNA YOHANNES SIMANULLANG; I NENGAH ARTHA; A. A. N. GEDE SUWASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.2, April 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Effect of Composition Planting Medium and Inorganic Compound Fertilizer application on The Growth of Early Oil Palm Seeds(Elaeis guineensis Jacq.) This study aims to: understand the interaction effect of composition planting medium and inorganic compound fertilizer application on the growth of early oil palm seeds (Elaeis guineensis Jacq). Research on the effect of composition planting medium and inorganic compound fertilizer using factorial randomized block design with two factors, namely: (planting medium, compost: soil: sand) which consists of four levels, namely: (0 compost: 1 soil: 1 sand), (1 compost: 1 soil: 1 sand), (2 compost: 1 soil: 1 sand), (3 compost: 1 soil: 1 sand), while the inorganic compound fertilizer application consists of three levels, namely: 0 g / polybag; 2.5 g / polybag; 5 g / polybag. Observations were made on plant height, stem diameter, leaf area, number of leaf chlorophyl, oven dry weight of shoots, roots oven dry weight, total dry weight of seedlings, shoot ratio, plant growth rate, and net assimilation rate. Data were analyzed by analysis of variance and if the real effect will be tested further with LSD 5%.The results of this study indicate that the interaction of composition planting media and inorganic compound fertilizers are not significant to the growth of early oil palm seeds (Elaeis guineensis Jacq). Single factor of planting medium and inorganic compound fertilizer is not significant to the growth of early oil palm seedlings (Elaies guineensis Jacq.). The highest value seen in the growing compost media composition: soil: sand (2: 1: 1), inorganic compound fertilizer highest value contained in a dose of 2.5 g / polybag on parameters of leaf area, leaf chlorophyll, dry root weight of the oven, and total weight of oven dried beans.
Aplikasi Beberapa Jenis Pupuk Organik Kemasan untuk Menekan Penyakit Akar Gada dan Meningkatkan Pertumbuhan Tanaman Kubis (Brassica oleracea L.) NI MADE PRITIYANTI DEWI; I MADE SUDANA; A. A. NGURAH GEDE SUWASTIKA; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Application Some Types of Organic Fertilizer in a Pack To Suppress Clubroots and Improve Cabbage (Brassica oleracea L.) Plant Growth Cabbage is one type of leaf vegetables from subtropics area that has been known for a long time and cultivated in Indonesia. One of the hardest cabbage diseases is clubroots caused by the Plasmodiophora brassicae pathogen. This pathogen can cause high damage to cabbage plants. The used of synthetic pesticides is less success in the implementation, and caused environmental pollution, especially the soil, this study tried to control clubroots in the cabbage by using 8 types of organic fertilizer in a pack. This study used a Completely Randomized Design (CRD) with 9 treatments including controls with three replications. The results obtained four best treatments that were effective in suppressing clubroots while increasing the leaf height, leaf amount, leaf area, and maximum dry weight of the plants, that three best treatments were organic fertilizer PSBN, Multitonik, and Biotonik. The best treatment to suppress clubroots is found in organic fertilizer PSBN, Multitonik, and Biotonik which has the lowest percentage of ulcer attack by 33,33-55,56% compared to control (100%).
Analisis Kualitas Kompos Limbah Upacara Agama Hindu di Denpasar dengan EM4 sebagai Dekomposer I MADE WERAYOGA; I WAYAN DANA ATMAJA; A A NGURAH GEDE SUWASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Analysis of the Compost Quality of Hindu Ceremony Wastein Denpasar with EM4 as a DekomposerThe aim of this research was to find out best quality of compost made of by the waste of Hindu ceremony in Denpasar, cow dung, and bran, with EM4 as a decomposer. The research was conducted on November 2014 until June 2015, located at pegok experimental field, faculty of agriculture, Udayana university. The design used was Randomized block design with two factors, the first factor was combination the waste of Hindu ceremony and the second factor was the decomposer consisted of without decomposer and with EM4 with dose of 200 mL (dilution 20 mL EM4 + 10 g sugar + 1 liter water). Compost quality was known by counting the total bacterial population, total fungi population, and respiration, measuring the temperature, water content, weight, structure, smell, color, measuring the content of total N, total P, total K, C-organic, the C/N ratio, and the pH. The result of research based on statistical analysis show that interaction impact between combination the Waste of Hindu ceremony and decomposer only have real influence on the parameter of total bacterial population, total fungi population, N-total, and P-total of compos. The research resulting in several conclusions that is the treatment of 75% the waste of Hindu ceremony + 15% cow dung +10%bran presenting the best quality of compost showed by the height of total bacterial population ( 17,38 x 108 cpu g-1 of compost), highest N-total (1,82%), the lowest content of C-organic (10,39%), highest P-total (83,71 mg/100g).
Analisis Kualitas Larutan Mikroorganisme Lokal Daun Gamal (Gliricidia sepium) pada Beberapa Waktu Inkubasi ANAK AGUNG NGURAH GEDE SUWASTIKA; NI WAYAN SRI SUTARI; NI WAYAN MURIANI
Agrotrop : Journal on Agriculture Science Vol 5 No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Analysis of Local Microorganism Leaf Gamal (Gliricidia Sepium) Solution Quality onthe Fermentation Period. The study aims to determine the quality of local microorganismsolution under the influence of concentration leaves of Gliricidia (Gliricidia sepium) andfermentation period. This research was conducted at the Soil Laboratory, Faculty ofAgriculture, UdayanaUniversity. The design of this research used a randomized block designfactorial pattern of two factors. The first factor was the concentration of Gliricidia leavesconsisted of 0, 100, 300 g, and the second factor was the fermentation period, consists of 1,2,and 3 weeks. Results of statistical analysis showed a highly significant interaction betweenthe concentration factor and fermentation period of Gliricidia leaves the parameters observed.Concentration of 30% (300 g) Gliricidia leaves with a two-week fermentation period has thebest effect on the quality of the biological properties of microorganisms local solution with atotal population of bacteria (9.5 x 107 SPK mL-1) and total fungus (1.9 x 106 SPK mL-1),while the best quality chemical properties of the solution of local microorganisms present in aconcentration of 30% (300 g) gliricidia leaves with a three-week fermentation period with thecontent of total-N (1.59%), pH (6.03), and organic-C (3.35%). Concentration of 30% (300 g)Gliricidia leaves with fermentation period of two weeks, it can be used as a biologicalfertilizer and a concentration of 30% (300 g) Gliricidia leaves with a three-week fermentationperiod, could be used as organic manure.
Pengaruh Ukuran Biochar Bambu dan Dosis Pupuk Urea terhadap Efisiensi Pupuk dan Hasil Tanaman Sawi Hijau (Brassica juncea L.) pada Tanah Berpasir ANAK AGUNG GDE PREHATRESNA ADI ASMARA; I WAYAN DANA ATMAJA; ANAK AGUNG NGURAH GEDE SUWASTIKA; ANAK AGUNG ISTRI KESUMADEWI
Agrotrop : Journal on Agriculture Science Vol 11 No 2 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i02.p05

Abstract

The Effect of Bamboo Biochar Size and Urea Fertilizer Dosage on Fertilizer Efficiency and Yield of Green Mustard (Brassica juncea L.) in Sandy Soil. Biochar is charcoal produced from incomplete combustion (carbonization). Biochar is a soil amendment agent that has long been known in agriculture which is useful for increasing soil productivity. This study aims to determine the effect of bamboo biochar size and urea fertilizer dosage on the efficiency of urea fertilizer and yield of green mustard (Brassica juncea L.). This research was conducted from May 2020 to September 2020 at the Experimental Garden of the Faculty of Agriculture, and at the Laboratory of Soil and Environmental Sciences, Faculty of Agriculture, Udayana University. The research design used was a completely randomized design (CRD) with 2 factorials and 3 replications. The treatment factors consisted of P0 = 0kg urea (control) (0 grams / pot), P1 = 100kg urea / ha (0.2 grams / pot), P2 = 200kg urea / ha (0.4 grams / pot), P3 = 300kg urea / ha (0.6 gram / pot), B1 = 0.25-2.00 mm, B2 = 2.38-2.83 mm, B3 = 2.83-3.36 mm. The parameters observed were levels of ammonium, nitrate, soil pH, microbial population, soil respiration, crop yields including fresh weight and oven dry weight of the plant. The results of the analysis showed that the effect of biochar and urea fertilizers interacted very significantly with the parameters of the microbial population and had no significant effect on the parameters of ammonium, nitrate, soil pH, soil respiration, plant fresh weight, plant dry weight, root fresh weight, root dry weight and efficiency. fertilizer. Biochar treatment has a significant effect on biological factors and fertilizer treatment affects soil chemical properties, crop yields are influenced by a single factor.
Dinamika Amonium dan Nitrat Pada Lahan Sawah Semi Organik untuk Tanaman Padi Lokal dan Hibrida di Subak Jatiluwih Kabupaten Tabanan KHOSNUL KHOTIMAH; ANAK AGUNG NGURAH GEDE SUWASTIKA; I WAYAN DANA ATMAJA
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.824 KB) | DOI: 10.24843/AJoAS.2020.v10.i01.p05

Abstract

Dynamics Of Ammonium And Nitrate In Semi Organic Rice Fields For Local And Hybrid Rice Plants In Subak Jatiluwih, Tabanan Regency. The study was conducted to determine the dynamics of ammonium and nitrate and time subsequent fertilization in paddy fields with local and hybrid rice. Soil and water samples in two replications were taken each the two paddy fields periodically, namely during the tillage (DTT), 0, 7, 14, 21, 28, 42, 56, 70, 84, 98, and 112 days after planting (DAP). Ammonium and nitrate levels were analyzed by Macro Kjeldahl method. The results showed that the dynamics of ammonium and soil nitrate were different in two rice varieties. The highest ammonium content of local rice at the age of 7 DAP rice and the highest nitrate content at age 14 DAP. Ammonium and nitrate levels are highest in hybrid rice fields at 28 DAP rice age. The lowest ammonium and nitrate levels in two different varieties of rice land, namely the lowest local rice varieties at the age 28 DAP, whereas in hybrid rice land at age of 98 DAP. The results of this study indicate that supplementation of urea can be carried out at the age of 21 and 98 DAP for local rice varieties, and at the age of 14 DAP and 42 DAP for hybrid rice varieties.
Co-Authors Aleksey Stepanov ANAK AGUNG GDE PREHATRESNA ADI ASMARA Anak Agung Istri Kesumadewi ARJUNA YOHANNES SIMANULLANG GITA YUNI PERMATASARI I Kadek Ngestika Pradnyana I Ketut Suada I MADE SUDANA I MADE WERAYOGA I NENGAH ARTHA I Wayan Dana Atmaja KHOSNUL KHOTIMAH Nataliya Shchegolkova NI MADE PRITIYANTI DEWI NI PUTU NENA LUSIANA NI PUTU SRI SUTARI NI WAYAN MARSININGSIH NI WAYAN MURIANI Oleg Gladkov Olga Yakimenko PUTU ANANTA WIDHIA DHARMA Rodion Poloskin