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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Caraka Tani: Journal of Sustainable Agriculture Advances in Tropical Biodiversity and Environmental Sciences Nandur

I Ketut Suada

Faculty Of Agriculture, Udayana University, Denpasar, Indonesia

Author-ID : 2928193

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Veterinary

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PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF JUWET FRUIT (Syzygium cumini L. Skeels) ETHANOLIC EXTRACT I Made Parama Nanda Mahatya; I Gede Putu Wirawan; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 8 No 2 (2021): International Journal of Bioscience and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v08.i02.p05

Juwet (Syzygium cumini L. Skeels) is one of Indonesia's local fruits which its existence is hard to find now. All parts of this plant can be used for many treatments, one of which is as an antioxidant. The purpose of this study was to determine the compounds contained in the fruit and to determine the antioxidant activity as well. Phytochemical analysis was performed using the gas chromatography while antioxidant activity tests were carried out quantitatively by DPPH method. The results showed that the juwet fruit was contained phenol 284.47 mg/100 g GAE, flavonoids 379.07 mg/100g, tannins 3888.67 mg/100 g TAE and anthocyanins 40.28 mg/100 g which consisted of 66 types of chemical compounds. The results of the antioxidant analysis showed that the ethanolic extract of juwet fruit could inhibit DPPH radicals with IC50 of 72,96 µg/ml.

ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE I Ketut Suada; Anak Agung Ngurah Gede Suwastika
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.

APPLICATION OF Trichoderma spp. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (Plasmodiophora brassicae WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE I Ketut Suada; Anak Agung Ngurah Gede Suwastika; I Kadek Ngestika Pradnyana; Nataliya Shchegolkova; Rodion Poloskin; Oleg Gladkov; Olga Yakimenko; Aleksey Stepanov
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%. This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.

SPORE PROPAGATION OF INDIGENOUS ENDOMYCHORIZA FROM SEVERAL ROOTING AREAS OF SNAKE FRUIT ON DIFFERENT SOIL WATER CONTENT I Nyoman Rai; I Ketut Suada; M. Praborini; I Wayan Wiraatmaja
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Cultivation of organic snake fruit in Bali which is done on dry land with the irrigation depends on rainfall and the fertilization generally only uses uncertain amount of fallen leaves, it reduces the productivity, quality, and continuity of fruit production over time. In rhizosphere of snake fruit there are various types of indigenous endomycorrhiza that potentially can be developed as biofertilizer, but as a biofertilizer, the low number of spores population for inoculum becomes a limiting factor in using indigenous endomycorrhiza. The effort that can be done are to propagate the spores by giving water stress treatment. This study aimed to obtain the best rhizosphere location of snake fruit as the source of spores and the level of soil water content to multiply the spores. The research was conducted in the green House, Farm Station, Agriculture Faculty, Udayana University at Jalan Pulau Moyo, Denpasar, from October 2017 to January 2018. The spore propagation used nested experiment with Randomized Block Design patterns. The treatments were rhizosphere location as the source of indigenous endomycorrhizas spore consists of three levels (Bebandem District Karangasem Regency, Payangan District Gianyar Regency, and Pupuan District Tabanan Regency) and soil water content consists of three levels (100%, 70%, and 40% field capacity). The results of the research showed that the source of indigenous endomychorrizae from snake fruit rhizosphere in Payangan District gave the highest number of spores found and the highest percentage of the spore increase after propagation. While in the soil water content treatments, the highest number of spores found and the percentage of the spore increase after propagation were obtained at soil water content of 40% field capacity. The percentage of root infections from different sources of indigenous endomychorrhizae and different levels of soil water content is same i.e 100%.

PRIMER DESIGN OF CVPDr DNA FRAGMENT SEQUENCES THAT AMPLIFY SPECIFIC FRAGMENTS TO DISTINCT THE RESISTANT FRAGMENT FROM Triphasia trifolia (Burm. F.) P. Wils. AND THE SUSCEPTIBLE FRAGMENT FROM Citrus nobilis Lour. Ni Made Ayuratih Utami; I Gede Putu Wirawan; I Ketut Suada
International Journal of Biosciences and Biotechnology Vol 7 No 2 (2020)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

CVPDr is a DNA fragment that indicates that plants are resistant to CVPD. Previous research using primers that amplified 841 bp CVPDr fragment was able to amplify the fragment from Triphasia trifolia that considers being a resistant plant, Citrus aurantifolia var. seedless which considers being a tolerant plant, and some susceptible citrus plants to CVPD disease. In this study, we designed some primers that amplified only CVPDr DNA fragment from T. trifolia which consider as the resistant plant and a primer that amplified only DNA fragmen from Citrus nobilis which consider as the susceptible citrus plants. The primers for CVPDr on T. trifolia are TCATCTGCATGGGATACC for forward primer and GCCTTGAGCTTGTAAGTG for reverse primer which turned out to amplify the DNA of T. trifolia and also the C. nobilis cultivar Denpasar and only succeeded in not amplifying the C. nobilis cultivar Gianyar. The primers for CVPDr on C. nobilis are GAATGGCTTAGCAGAAAGG for forward primer and GGTTGTAGATGGACATAGG for reverse primer turned out can not only amplify the DNA C. nobilis but also amplify T. trifolia.

Upaya Meningkatkan Produktivitas Sapi Bali Melalui Pengendalian Penyakit Parasit di Sekitar Sentra Pembibitan Sapi Bali di Desa Sobangan I. A. P. Apsasri; I.B.N. Swacita; I. B. Ardana; G.A.Y. Kencana; I K. Suada
Buletin Udayana Mengabdi Vol 15 No 1 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.263 KB)

This dedication aims to improving the productivity of Bali cattle through the control of parasites diseases at around Bali cattle breeding center in the village Sobangan. This service activities preceded the survey in cattle around Bali cattle breeding center in the village Sobangan, regarding the data of infection and illness. The method is applied for the attainment of the objectives of this activity is the administration of worms drugs directly to the breeder in around the Bali cattle breeding centers in Sobangan and extension of the maintenance management of cattle to prevent infection by the parasite. Service activities in the form of health services in Bali cattle was held on June 27th, 2015 in the village Sobangan Mengwi Badung Bali. These activities responded very well by livestock farmers around the Bali cattle breeding center in the village Sobangan. Conclusions dedication to the community around the Bali cattle breeding center in the village Sobangan has succeeded well implemented. Suggestions that the provision of worms drug in cattle regularly given, so that cattle can continue to increase the productivity.

SOSIALISASI PENYAKIT ZOONOSIS ESCHERICHIA COLI O157:H7 SERTA PELAYANAN KESEHATAN SAPI DI DUSUN LAMPU DESA CATUR KINTAMANI BANGLI I W. Suardana; I.B.N. Swacita; I.N. Suartha; I G.N. Sudisma; M.D. Rudyanto; I.G.M. Krisna Erawan; I.N. Suarsana; I.W. Batan; P.A. Sisyawati Putriningsih; T. Sari Nindia; A.L.T. Rompis; I.N. Mantik Astawa; K. Karang Agustina; I.H. Utama; I.G.A. Suartini; I.M. Sukada; I.K. Suada; A.A.A. Mirah Adi
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.738 KB)

Ternak sapi yang menderita diare berpeluang besar untuk ditemukan adanya agen zoonosis E. coli O157:H7 mengingat sapi sebagai reservoir utama dari agen tersebut.Transmisi penularan strain bakteri ini ke manusia umumnya terjadi melalui konsumsi daging yang kurang dimasak, produk susu yang tidak dipasteurisasi, air yang terkontaminasi feses. Dusun Lampu sebagai salah satu Dusun di Desa Catur merupakan salah satu daerah potensial untuk pengembangan ternak khususnya sapi sehingga menjadikan program pelayanan kesehatan di wilayah tersebut sangat potensial untuk dilakukan. Hasil kegiatan pengabdian masyarakat berupa sosialisasi penyakit zoonosis E. coli O157:H7 serta pelayanan kesehatan ternak sapi di Dusun ini, memperlihatkan respon positif yang dicirikan dengan cukup banyaknya jumlah ternak yang memperoleh pelayanan yaitu sejumlah 65 ekor sapi dari 35 petani ternak. Jenis pelayanan yang dilakukan meliputi tindakan spraying atau pemberian butox terhadap semua ternak sapi yaitu 65 ekor (100%), disusul dengan pemberian vitamin pada 52 ekor (80%), pemberian obat cacing sebanyak 39 ekor (60%), serta pemberian delladryl pada 1 ekor sapi (1,5%). Hasil ini mengindikasikan bahwa program pengabdian yang dilakukan cukup efektif dapat menyentuh kebutuhan dasar petani ternak, sehingga benar-benar dapat dirasakan manfaatnya.

PENERAPAN PUPUK HAYATI PADA TANAMAN PADI DI DESA TEGALMENGKEB KECAMATAN SELEMADEG TIMUR KABUPATEN TABANAN BALI A.A.N.G. Suwastika; I.K. Suada; A.A.A.A.S. Sunari; N.W.S. Sutari
Buletin Udayana Mengabdi Vol 17 No 1 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Community service is implemented in Tegalmengkeb Village of East Selemadeg District Tabanan, Bali. Thepurpose of this dedication improves the understanding and skills of farmers in the application of biofertilizeron rice crops. The method used in this activity is counselling with lectures and discussions, followed bytraining on the manufacture of local microorganisms (MOL) and the application of biofertilizer on rice crops.It is hoped that the community can apply biofertilizer technology that can improve rice crop yield andfarmer's income. The results of the training increase the understanding of farmers about biofertilizer by 30%,know about the biofertilizer and the role of biofertilizer as fertilizer of soil and plants. Increased farmers'skills in the production of local microorganisms (MOL) and can apply Egary biofertilizer to rice crops.

KERAGAMAN AKTIVITAS ANTIFUNGI BIOTA LAUT TERHADAP Fusarium oxysporum f.sp. vanillae, PENYEBAB BUSUK BATANG VANILI I Ketut Suada
Bumi Lestari Journal of Environment Vol 12 No 1 (2012)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

The diversity of marine biota resources is very high, therefore it is necessary to be recovered for our life need. The objective of this research is to know the antifungal ability of marine biota derived from Bali Island against Fusarium oxysporum f. sp. vanillae. Samples were collected at the intertidal zone of seven beaches around Bali. Extraction of antifungal substance of raw material extract was conducted using various organic solvents until the best ability was obtained. The dry material extract was then screened using well diffusion method. The method was also used to determine the inhibition indicators to Fusarium. The methanolic extract of Aglaophenia sp. marine animal was able to suppress the Fusarium effectively, with minimum inhibition concentration (MIC) of 0.05%. The extract inhibited the colony growth, total of conidial forming, total of growing colony, total of mycelial dry weight, total of mycelial protein, however, increased fusaric acid production of the pathogen.

Uji Aktivitas Antijamur Ekstrak Beberapa Jenis Biota Laut terhadap Aspergillus flavus LINK dan Penicillium sp. LINK SANGGUL HUTASOIT; I KETUT SUADA; I GEDE SUSRAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.433 KB)

ABSTRACT Study of Anti fungi Extract of Some Types of Marine Biota to the Apergillus flavus LINK. and Penicillium sp. LINK. The purpose of this study is to know the effectiveness of marine biota, namely Aglaophenia sp., Eucheuma cottonii, Sargassum sp., Gracilaria sp., and Ulva sp. in impeding the growth of Aspergillus flavus and Penicillium sp. fungi which is isolated from contaminated corn seed. The results of this study shown that the crude extract of marine biota is very effective in impeding the growth of Aspergillus flavus and Penicillium sp. fungi. Extract which is capable forming the greater impeding zone is Aglaophenia sp, that is 68,20 mm to Penicillium sp. with very strong category of impeading power. Aglaophenia sp. ekstract shown a greater empeading power to the colony of fungi, that is 49,40% to Aspergillus flavus and 49,20% to Penicillium sp. Keywords: Marine Biota, Anti fungi, Aspergillus flavus, and Penicillium sp.

Co-Authors A.A.A.A.S. Sunari A.A.N.G. Suwastika Aida Lousie Tenden Rompis Aleksey Stepanov Anak Agung Ngurah Gede Suwastika ANDRIANI ANDRIANI DELVIANA PANJAITAN Dewa Ayu Ari Febriyanti Dewa Ngurah Suprapta DWI SURYANTO EUSEBIO GOMES Fransen Sinaga GEDE WIJANA Gusti Ayu Yuniati Kencana HERLINDAH CHOIRI I Gede Ketut Susrama I GEDE PUTU WIRAWAN I GEDE SUSRAMA I Gusti Agung Ayu Suartini I Gusti Ayu Diah Yuniti I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I Kadek Ngestika Pradnyana I KADEK NGESTIKA PRADNYANA I MADE AGUS WIARTANA I Made Ary Putra Nata I Made Ary Putra Nata I Made Merdana I Made Parama Nanda Mahatya I MADE SUDANA I MADE SUDANA I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I Made Sukada I Nyoman Adi Khrisna Wijaya I NYOMAN MANTIK ASTAWA I NYOMAN RAI I Nyoman Suarsana I Nyoman Suartha I Putu Agus Santika Putra I WAYAN BUDIASA I WAYAN EKA ADI WIRAWAN I Wayan Suardana I WAYAN WIRAATMAJA I. A. P. Apsasri I. B. Ardana I.H. Utama I.W. Batan IDA AYU GEDE DIANGGI ADIATHY Ida Bagus Ngurah Swacita IRMA SELVYANA Br. SITEPU KADEK INTAN SURYANINGSIH Kadek Karang Agustina KETUT AYU YULIADHI M. Praborini M.D. Rudyanto MADE PHARMAWATI MADE SRITAMIN MARLIN MARHAENI PE N.W.S. Sutari Nataliya Shchegolkova NI KADEK LIA SWANDEWI Ni Komang Alit Astiari NI LUH GEDE SRIYANTI NI LUH PUTU SONIA SAVITA DEWI Ni Made Ayuratih Utami NI MADE PRITIYANTI DEWI Ni Made Puspawati Ni Made Savita Rasjman Rasjman NI MADE TRIGUNASIH NI NYOMAN ARI MAYADEWI NI PUTU LINDA SUNARIASIH NI PUTU MERRY SENI ANTARI Ni Putu Ratna Dewi Damayanti NI WAYAN SUNITI Oleg Gladkov Olga Yakimenko Onesimus Ke Lele PUTU AYU MEITA YUDIA DEWI Putu Ayu Sisyawati Putriningsih PUTU SUGITA Rodion Poloskin SANGGUL HUTASOIT SHEILA SIMANJUNTAK T. Sari Nindia TRISNA AGUNG PHABIOLA VEDERIS LEUNARDUS VICKY TANDYA WAYAN ADIARTAYASA

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