This retrospective study was conducted to know the relation between time and frequency of mating on litter size of Golden Retriver in Denpasar Region. Mating incident records of 19 Golden Retrievers from 8 kennels were compiled to be analyzed. These data can be use as a reference in effort to maximize the amount of Golden Retriever clans. The result showed that the three times of mating on the 10th , 12th and 14th day after onset of proestrus produced the most litter size 10 ± 2.82. However, mating twice on wice on 9th and 11th after onset of proestrus produced a litter size of 8.50 ± 0.70. The statistic analysis depicted that there was no significant relationship between mating frequencies and mating time on litter size.
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