JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI
Vol 7 No 1 (2019): Maret

ISOLASI BAKTERI SELULOLITIK PENDEGRADASI SELULOSA DARI KOMPOS

Zainul Arifin (Mahasiswa Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)
Ida Bagus Wayan Gunam (Dosen Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)
Nyoman Semadi Antara (Dosen Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Unud)
Yohanes Setiyo (Dosen Program Studi Teknik Pertanian, Fakultas Teknologi Pertanian, Unud)



Article Info

Publish Date
02 Apr 2019

Abstract

This study aimed to isolate cellulolytic bacteria that have the potential to degrade cellulose taken compost samples from Temesi and Bondowoso, this study was also conducted to determine the ability of bacteria to degrade cellulose based on Congo red test and filter paper degradation test. All isolates were tested for cellulolytic activity using soluble Carboxymethyl Cellulose (CMC). Characterization was carried out by growing selected pure isolates on CMC media and then dripping 0.1% congo red to test the cellulolytic potential (cellulolytic potential was characterized by the emergence of clear zones around the colony). The results of isolation of bacteria obtained 38 isolates, namely 26 Bondowoso sample isolates and 12 Temesi isolates that were able to grow and utilize cellulose as a carbon source. But only fourteen isolates produced clear zones in the Congo red test with diameters ranging from 1.66 cm to 6.76 cm. Six isolates that have the largest diameters clear zone, were tested for degradation of filter paper (Whatman no. 1). Isolates bacteria of B2S8 obtained from Bondowoso compost samples has the highest ability to degrade cellulose on Whatman paper no. 1 as much as 51.30%. Keywords: Compost, Isolation, Screening, Cellulolytic Bacteria.

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Journal Info

Abbrev

jtip

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI adalah media publikasi ilmiah yang diterbitkan oleh Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian-Universitas Udayana, berisikan artikel hasil-hasil penelitian, ulasan (review), opini ilmiah oleh mahasiswa, dosen, praktisi, dan ...