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All Journal IPTEKMA Agrotekno E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) agriTECH Indonesian Journal of Chemistry Agroindustrial Journal Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Jurnal Ilmiah Teknologi Pertanian Agrotechno
NYOMAN SEMADI ANTARA
Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Bukit Jimbaran-Bali.
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences

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PRODUKSI BUBUK INOKULUM URUTAN DARI KULTUR MURNI Pediococcus acidilactici U318 DENGAN BEBERAPA JENIS BAHAN PENGISI Semadi Antara, Nyoman; Urip Sanjaya Trisna, I P. G.; Suter, I Ketut
Agrotekno Vol. 15, No. 1 Februari 2009
Publisher : Universitas Udayana

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Abstract

Aim of the research was to find out the viability ofPediococcus acidilactici U318 in inoculum powder duringstorage. The filler material used in the production ofinoculum powder was three kinds of flour, namely maizena,rice flour and tapioca. The research was done in laboratoryscale as experiment research which was designed byrandomized block design with factorial experiment. Twofactors were experimented, namely the kinds of flour as afiller material as the first factor and the storage period as thesecond factor.Result of the research showed that the treatment of flouras a filler material did not influence the viability of P.acidilactici U318 in inoculum powder. Any filler materialused in production of the powder gave same protective actionon its viability. The viability of P. acidilactici U318decreased during storage period. After storage of 4 weeks theaverage of viable P. acidilactici U318 was about 2,54x1010cfu/g which decreased one log cycle from the initial ofstorage.
TINGKAT CEMARAN BAKTERI COLIFORM, SALMONELLA SP., DAN STAPHYLOCOCCUS AUREUS PADA DAGING BABI (STUDI KASUS RUMAH POTONG HEWAN SANGGARAN DAN PASAR BADUNG, BALI Semadi Antara, Nyoman; Djaya Utama Dauh, Ida Bagus; Ita Seri Utami, Ni Made
Agrotekno Vol. 14, No.2 Agustus 2008
Publisher : Universitas Udayana

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Pengaruh Ukuran Partikel dan Suhu Ekstraksi Terhadap Karakteristik Ekstrak Pewarna Alami Bunga Kenikir (Tegetes erecta L.) Purnama Yanti, Gusti Ayu Kade Diana; Wartini, Ni Made; Antara, Nyoman Semadi
IPTEKMA Volume 8, No. 1, Februari 2019
Publisher : Bidang Kemahasiswaan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/iptekma.2019.v08.i01.p03

Abstract

PENGARUH UKURAN PARTIKEL DAN SUHU EKSTRAKSI TERHADAP KARAKTERISTIK EKSTRAK PEWARNA ALAMI BUNGA KENIKIR (TEGETES ERECTA L.). Bunga kenikir marigold berpotensi sebagai sumber pewarna alami. Warna kuning hingga oranye diperoleh melalui proses ekstraksi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ukuran partikel dan suhu ekstraksi terhadap ekstrak pewarna alami bunga kenikir serta menentukan perlakuan terbaik untuk mendapatkan ekstrak pewarna alami bunga kenikir marigold. Rancangan dalam penelitian ini menggunakan Rancangan Acak Kelompok dengan dua faktor. Faktor I adalah perlakuan ukuran partikel yang terdiri dari tiga taraf yaitu 40, 60, dan 80 mesh. Faktor II adalah perlakuan suhu ekstraksi yang terdiri dari tiga taraf yaitu 30±2 ºC, 45±2 ºC, dan 60±2 ºC. Hasil penelitian menunjukkan bahwa perlakuan ukuran partikel dan suhu ekstraksi berpengaruh sangat nyata (P<0,01) terhadap rendemen, total karotenoid, tingkat kecerahan (L*), tingkat kemerahan (a*), dan tingkat kekuningan (b*). Interaksi kedua perlakuan berpengaruh sangat nyata (P<0,01) terhadap rendemen, total karotenoid, tingkat kecerahan (L*), tingkat kekuningan (b*) dan berpengaruh nyata (P<0.05) terhadap tingkat kemerahan (a*). Ukuran partikel 60 mesh dan suhu ekstraksi 45±2 ºC merupakan perlakuan terbaik untuk menghasilkan ekstrak pewarna bunga kenikir dengan karakteristik rendemen 8,79%, kadar total karotenoid 21,52%, tingkat kecerahan (L*) 21,05, tingkat kemerahan (a*) 11,43, dan tingkat kekuningan (b*) 19,66.
Karakterisasi Bakteri Asam Laktat yang Diisolasi dari Susu Kuda Bima Nyoman Semadi Antara; I Nyoman Dibia; Wayan Redi Aryanta
agriTECH Vol 29, No 1 (2009)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.361 KB) | DOI: 10.22146/agritech.9754

Abstract

The aim of the research was to characterize of the six species of Lactic Acid Bacteria (LAB) indigenous (Lactobacillus acidophilus KBc, Lb. salivarius KBd, Lb. brevis KBa, Lb. delbrueckii KBe, Lb. plantarum KBb and Lactococcus lactis KBf) isolated from the fermented horse milk of Bima. The tests were done to cover safety aspect, ability to survive on low pH condition, bile salt tolerance, ability to adherence and colonization, and effect on cholesterol level reduction. The six LAB species were not pathogen and invasive, and were able to grow well on media which contained bile salt of 0.75 %. Lactobacillus  acidophilus KBc and Lb. brevis KBa had ability to survive on media pH 2.5 for 3 hours and were able to adherence and colonization on gut mucosa epithel. Lactobacillus acidophilus KBc and Lb. brevis KBa had ability to reduce cholesterol level of blood serum of rabbits on hypercholesterolemia condition significantly up to 53.74 % and 51.70 %, respectively. Based on the characterization of the six species LAB, only Lactobacillus acidophilus KBc and Lb. brevis KBa can be used as probiotic.ABSTRAKPada penelitian ini telah dilakukan karakterisasi terhadap enam spesies bakteri asam laktat  (BAL) indigenous (Lac- tobacillus acidophilus KBc, Lb. salivarius KBd, Lb. brevis KBa, Lb. delbrueckii KBe, Lb. plantarum KBb dan Lac- tococcus lactis KBf) yang diisolasi dari susu kuda liar Bima terfermentasi. Pengujian yang dilakukan meliputi aspek keamanan, ketahanan hidup pada media pH rendah, kemampuan tumbuh pada media yang mengandung garam em- pedu, kemampuan menempel dan berkolonisasi, dan efek terhadap penurunan kadar kolesterol serum darah. Keenam spesies BAL tersebut tidak patogen dan invasif, serta dapat tumbuh baik pada media yang mengandung garam empedu 0,75 %. Lactobacillus  acidophilus KBc dan Lb. brevis KBa tahan hidup pada media pH 2,5 selama 3 jam serta dapat menempel dan berkolonisasi pada epitel mukosa usus. Kedua bakteri ini mampu menurunkan kadar kolesterol serum darah kelinci dalam keadaan hiperkolesterolemia secara nyata masing-masing sebesar 53,74 % dan 51,70 %. Berdasar- kan karakterisasi keenam spesies BAL tersebut, Lb. acidophilus KBc dan Lb. brevis KBa dapat dipergunakan sebagai probiotik.
Uji Efektivitas Konsentrasi Minyak Atsiri Sereh Dapur (Cymbopogon Citratus (DC.) Stapf) terhadap Pertumbuhan Jamur Aspergillus Sp. secara In Vitro MARIA ULFA ELLA ELLA; KETUT SUMIARTHA SUMIARTHA; NI WAYAN SUNITI; I PUTU SUDIARTA; NYOMAN SEMADI ANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT In Vitro Study of the effectiveness of Essential Oil Extracted from Lemongrass (Cymbopogon citratus) to Aspergillus Sp. Study on determining of the effectivenes of essential oil extracted from lemongrass (Cymbopogon citratus) in order to suppressing the growth of fruit rot fungi (Asoergillus sp.) and also to determining the minimum concentration that able to inhibit colony growth and spore formation of the Aspergillus sp. in vitro, was conducted in Laboratory of Plant Diseases from June into November 2012. The result showed that the fungi were isolated from rot fruit mango has similarities with the fungi Aspergillus sp. The characteristic of Aspergillus has cepted hyphae, growth appear colonies, conidiophores uncepted and stand up, the tip of conidiophore is swollen and produce vesicle form, the surface of vesicle is covered by fialid that usually simple and colored or colorless, fialid produced conidia which were form green, brown or black chain. Essential oil of lemongrass have an antifungal activity and it was found effective to inhibit growth of the fruit rot fungi Aspergillus sp. on PDA. The inhibition zone of the lemongrass essential oil against Aspergillus sp. were 85 mm which is include a very strong category. Effective concentration of essential oil of lemongrass to inhibit the growth of Aspergillus sp. are 0,6% - 1%. The persentase of inhibition in this concentration reached to 100%. On the other hand, the control treatment, 0,2%, and 0,4% concentration were 0%, 0%, and 28,53%, respectively. Minimum concentration which is capable to inhibit the formation of spores and growth of fungi colonies were 0,2% and 0,3% concentration. The compounds suspected as antifungal against Aspergillus sp. were ?-citral (geraniol) and ?-citral (neral). Keywords: Lemongrass (Cymbopogon citratus), essential oil, antifungi, Aspergillus sp.
Utilization of Essential Oil of Lemongrass (Cymbopogon citratus) as a Bio-pesticide to Control Plutella xylostella (Lepidoptera: Plutellidae) I PUTU SUDIARTA; KETUT SUMIARTHA; NYOMAN SEMADI ANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRAK Pemanfaatan Minyak Atsiri Sereh Dapur (Cymbopogon citratus) sebagai Biopestisida untuk Mengendalikan Plutella xylostella (Lepidoptera: Plutellidae) Informasi tentang efikasi dan pitotoksisitas dari minyak atsiri sereh dapur sangat diperlukan dalam memanfaatkan minyaknya untuk mengendalikan Plutella xylostella. Penelitian efikasi minyak sereh di laboratorium sudah dilakukan sebagai penelitian pendahuluan sebelum melakukan penelitian di lapangan. Penelitian lapangan yang bertujuan untuk mengetahui efikasi dan pitotoksisitas minyak sereh dapur dilakukan di Desa Pancasari, Kecamatan Sukasada, Kabupaten Buleleng (1200 m dpl). Hasil penelitian menunjukan, minyak atsiri sereh dapur pada konsentrasi 10 % menimbulkan gejala terbakar pada daun kubis. Sedangkan pada konsentrasi 2.5 % dan 5 % menunjukan gejala perubahan warna dari hijau menjadi ungu. Tetapi pada konsentrasi rendah 1 %, 0.5 %, dan 0.1 % minyak sereh tidak memiliki pengaruh pitotoksik terhadap tanaman kubis. Pengaruh minyak sereh terhadap populasi P. xylostella menunjukan tidak beda nyata pada konsentrasi rendah. Tetapi pada umumnya dapat menekan populasi P. xylostella dibandingkan dengan kontrol. Berdasarkan penelitian tersebut maka minyak sereh dapur dapat dimanfaatkan sebagai pengendali P. xylostella pada konsentrasi rendah yaitu dibawah konsentrasi 1 %, karena pada konsentrasi tersebut minyak sereh tidak bersifat pitotoksik dan mampu menekan pupulasi P. xylostella di laboratorium maupun di lapangan. Kata kunci: minyak atsiri sereh dapur, Plutella xylostella, efikasi, pitotosisitas
Pengaruh Ketinggian Tempat, Mulsa dan Jumlah Bibit Terhadap Pertumbuhan dan Rendemen Minyak Sereh Dapur (Cymbopogon Citratus) I WAYAN HENDRA KUSUMAYADI KUSUMAYADI; I MADE SUKEWIJAYA SUKEWIJAYA; I KETUT SUMIARTHA; NYOMAN SEMADI ANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Aktivitas Antimikroba Ekstrak Bubuk Buah Mengkudu (Morinda citrifolia L.) Terhadap Pertumbuhan Bakteri Patogen Nyoman Semadi Antara; Vinnod Gema Prabanca; IGusti Ayu Ekawati
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 1, No. 1, 2014
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Penelitian yang dilakukan bertujuan untuk mengkaji aktivitas antimikroba ekstrak bubuk buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan beberapa bakteri patogen. Bubuk buah mengkudu diolah dari buah mentah dan matang yang kemudian diekstrak menggunakan berbagai jenis larutan pengekstrak. Larutan pengekstrak yang dicoba dalam penelitian ini adalah etanol, petroleum eter (PE), dan aquades. Hasil percobaan menunjukkan bahwa ekstrak bubuk mengkudu mentah dan matang yang diekstraksi dengan pelarut etanol memperlihatkan spektrum penghambatan yang lebih luas dibandingkan dengan menggunakan larutan pengekstrak PE dan aquades. Ekstrak buah mengkudu yang menggunakan etanol sebagai larutan pengekstrak dapat menghambat pertumbuhan Escherichia coli, Salmonella typhi dan Bacillus cereus. Sementara ekstrak menggunakan pelarut PE hanya memberikan penghambatan terhadap B. cereus, dan ekstrak bubuk mengkudu yang diekstrak dengan aquades tidak terdeteksi memberikan penghambatan terhadap ketiga bakteri uji.Kata Kunci: antimikroba, mengkudu, larutan pengekstrak, bakteri pathogen.
Microbiological And Physicochemical Changes Of Green Coffee (Coffea Arabica) Fermentation In Kintamani, Bangli, Bali Sayi Hatiningsih; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 5 No 2 (2018): Scientific Journal of Food Technology (September)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

The excellent quality of the fermentation process is closely related to microbes involved in the process fermentation. This study aimed to compare the microbiological changes and physicochemical quality of green coffee during 16 hours fermentation. Lactic acid bacteria and yeast were the predominant microorganisms found throughout the fermentation process. The total lactic acid bacteria and yeast reached the highest value of 9.31 log10 CFU/g and 9.10 log10 CFU/g respectively on the coffee bean and 9.30 log10 CFU/g and 9.10 log10 CFU/g respectively on liquid of fermented coffee bean. The quantity of microflora on the coffee bean and liquid of fermented coffee bean increased and reached its maximum value after twelve hours and decreased after exceed its peak, while fungi was no detected during fermentation. Sixteen hours fermentation significantly (P<0.05) influenced the bulk density, moisture content and bean weight/100 beans, bean amount/10 g and cause defect to green coffee such us “broken beans”, “brown beans” and “partly black beans” yet were not significantly affected (P>0.05) bean size and color of the green coffee. Throughout coffee fermentation, the acids on the green coffee increased from 5.33 ± 0.144 to 9.99 ± 0.144% and the caffeine on the green coffee decreased from 3.70 ± 0.017 to 1.66 ± 0.003%. Phenol content on the green coffee and its brews also decreased successively from 1381.58 ± 8.40 to 980.16 ± 10.07 mg GAEs/100 g and 922.25 ± 17.961 to 812.06 ± 12.660 mg GAEs/100 g. While for the antioxidants capacity, green coffee was more stable and its brews increased successively from 11564.28 ± 255.345 to 112 34.428 ± 174.377 mg GAEAC/L and 32326.24 ± 22.744 to 38658.62 ± 180.107 mg GAEAC/L. It can be concluded that coffee fermentation had more a positive effect on microbiological changes and physicochemical quality of green coffee.
KEMAMPUAN BAKTERI ASAM LAKTAT YANG DIISOLASI DARI AIR SUSU IBU DALAM MENURUNKAN KADAR KOLESTEROL DARAH TIKUS PUTIH GALUR WISTAR Dwi Ariesta Wijaya Putra; Semadi Antara; Komang Ayu Nocianitri
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Bile salt hydrolase (BSH) is one of the enzymes produced by lactic acid bacteria. This enzyme has theability to lower cholesterol levels in vitro and in vivo. The lactic acid bacteria used in this study wereL. plantarum I A1, L. plantarum I A8, L. plantarum I B8, L.rhamnosus A6 and L. rhamnosus B10b whicha probiotic candidate isolated from breast milk. The purpose of this study was to determine the activityof BSH and the ability to lower cholesterol both in vitro and in vivo. Three stages of experiment weredone. BSH activity and its ability in decreasing cholesterol in vitro were done for all strains ofLactobacillus. The best strain is L. plantarum I B8 was continued for further experiment to determineits ability to decrease blood cholesterol in in vivo using Wistar Rats.In vivo test results showed that L.plantarum I B8 decreased total cholesterol, LDL, triglyceride, and HDL by 27%, 21%, 29% and 35%respectively.
Co-Authors A.A.S. A. Sukmaningsih Ahmad Ali Akbar Ustadi Sadali Aimar, Ilhan Maulana Amna Hartiati Amna Hartiati Anak Agung Made Dewi Anggreni ANAK AGUNG MADE DEWI ANGGRENI Anak Agung Putu Agung Suryawan Wiranatha Apsari, Ni Putu Somya Anindita Arfira Khofifah Awang Bagaskara Ayu Putu Sarasdewi Bambang Admadi Harsojuwono Dwi Ariesta Wijaya Putra Dwi Ayu Kirani Paramita Dwi Putri Nurmalasari Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia Febriyawati Cahyanty Nugraha Febriyawati Cahyanty Nugraha Fitra Ayu Sitanggang Friska Ekagia Ginting G.P Ganda Putra G.P. Ganda Putra Gede Asstaradi Kusuma Gusti Putu Agus Darmataba Harsujuwono, Bambang Admadi I Dewa Gde Mayun Permana I Dewa Made Sukrama I Gede Arya Sujana I Gusti Agung Teguh Gardipa I Gusti Ayu Ekawati I Gusti Ayu Lani Triani I Gusti Ayu Lani Triani I Kadek Aditya Wirajaya I Ketut Satriawan I KETUT SUMIARTHA I Ketut Suter I Ketut Suter I M. Mahaputra Wijaya I Made Arif Sukmawan I Made Mahaputra Wijaya I Made Sukewijaya I Made Yoga Saputra I Nengah Kencana Putra I Nengah Widedianto I Nyoman Dibia I P. G. Urip Sanjaya Trisna I Putu Hendra Prasetya I Putu Sudiarta I W.G. SEDANA YOGA I Wayan Adi Wagestu I Wayan Arnata I WAYAN HENDRA KUSUMAYADI KUSUMAYADI I Wayan Suardana I Wayan Weta I Wayan Widia I. M. Mahaputra Wijaya Ida Ayu Ary Widnyani Ida Ayu Ketut Ariningsih Ida Bagus Djaya Utama Dauh Ida Bagus Wayan Gunam Ida R Hasibuan Irawaty Yolanda Hutajulu Kadek Dedi Widnyana Dinata Ketut Agus Ary Subakti Ketut Ratnayani KOMANG AYU NOCIANITRI Lilis Banne Paseru Luh Gede Mening Lestari Luh Putu Wrasiati Lutfi Suhendra MARIA ULFA ELLA ELLA Muhammad Najri Mulyani Sri Ni Kadek Maya Isadora Ni Kadek Wiji Astuti Ni Made Ita Seri Utami Ni Made Wartini Ni Nyoman Puspawati Ni Putu Suwariani NI WAYAN SUNITI Nurul Lita Ajizah Nurul Octavia Wasis Pande Ketut Diah Kencana Pradnyani, Putu Dhea Purba, Ira Sylvia Purnama Yanti, Gusti Ayu Kade Diana Putra, I Gede Adi Darmaja Putri Cininta Giskha Putu Agus Nadiarta Putu Agus Nadiarta R. Ag. Ay. Ag. Bayu Chandraliawathy Randall Howbert Rani Situmorang Rendy Sinaga Rian Lamhot Parasian Hutabarat Richard Howard Patty Rike Pratiwi Risky Simarmata Risky Simarmata Sally, Calvin Eliezer Immanuel Saragih, Davina Patricia Sarah Yohana Sihombing Sayi Hatiningsih Siva Safera Hajiani Sri Suhartini Sukoco, Hendro Suwariani , Ni Putu Suwariani, Ni Putu Tuti Herlina Br.Naibaho Umi Fadilah Vinnod Gema Prabanca Wayan Redi Aryanta Yan Ramona Yeni Veronika Simatupang Yohanes Setiyo Zainul Arifin Zulkifly Al Haq