Jurnal Farmasi Udayana
Vol. 1, No. 1, Tahun 2012

UJI AKTIVITAS PENANGKAPAN RADIKAL DPPH EKSTRAK PRODUK TEH HITAM (Camellia sinensis (L.) O.K.) DAN GAMBIR (Uncaria gambir (Hunter) Roxb) SERTA PROFIL KLT-DENSITOMETERNYA

Jaya, I. G. N. I. P (Unknown)
Leliqia N. P. E (Unknown)
I.N.K. Widjaja (Unknown)



Article Info

Publish Date
04 Mar 2013

Abstract

The purpose of this research was to compare the DPPH scavenging activity of black tea (Camellia sinensis (L.) OK) and gambier (Uncaria gambir (Hunter) Roxb) product extract and to determine their TLC-densitometer profile. This research was conducted in two stages, first stage was to examine the DPPH scavenging activity of black tea and gambier product extract. This assay used some concentrations of black tea product extract, gambier product extract, and standard vitamin C (10, 20, 40, 80, 160, 200, and 320 µg/mL). The second stage was to determine the extracts profile by TLC-densitometer. The result of this research showed that the DPPH scavenging activity of gambier product extract was greater (IC50 = 88,57±0,64 µg/mL) than black tea product extract (IC50 = 311,54±2,79 µg/mL). The result of profile determination of black tea product extract at a mobile phase of MeOH:toluene (50:50) showed that no spot was supposed a volatile oil group; at mobile phase of ethyl acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was supposed a flavonoid group, spot with Rf 0,41 (?max 365 nm) and spot with Rf 0,97 (?max 280 nm); at mobile phase of CHCl3:MeOH (90:10) showed that spot with Rf 0,87 (?max 275 nm) was supposed an alkaloid group; at mobile phase of acetic acid:diethyl ether:n-hexane:ethyl acetate (20:20:20:40) showed that spot with Rf 0,62 (?max 290 nm) was supposed a tannin group. While the result of profile determination of gambier product extract at mobile phase of ethyl acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was supposed a flavonoid group, spot with Rf 0,81 (?max 280 nm) and spot with Rf 0,92 (?max 280 nm); at mobile phase of acetic acid:diethyl ether:n-hexane:ethyl acetate (20:20:20:40) showed that spot with Rf 0,42 (?max 285 nm) was supposed a tannin group.

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Journal Info

Abbrev

jfu

Publisher

Subject

Medicine & Pharmacology

Description

Jurnal Farmasi Udayana merupakan jurnal elektronik yang dikelola oleh jurusan Farmasi FMIPA Udayana. Jurnal ini yang merupakan media publikasi penelitian dan review article pada semua aspek ilmu farmasi yang bersifat inovatif , kreatif, original dan didasarkan pada scientific. Artikel yang dimuat ...