JOURNAL OF COASTAL DEVELOPMENT
Vol 6, No 3 (2003): Volume 6, Number 3, Year 2003

CHARACTERIZATION AND IDENTIFICATION OF STRAIN KM221, A NOVEL MCPA HERBICIDE-DEGRADING BACTERIUM ISOLATED FROM CORAL SURFACE, MENJANGAN KECIL ISLAND, KARIMUNJAWA

Agus Sabdono (Unknown)
Ocky Karna Radjasa (Unknown)
Joedoro Soedarsono (Unknown)



Article Info

Publish Date
30 Jul 2013

Abstract

In this study, bacterial strain KM221 was isolated from coral   tissue in Menjangan Kecil Island, Karimunjawa, Indonesia. This strain is  facultative anaerobic  with MCPA (2-methyl-4-chlorophenoxy acetic acid) serving as the only known energy sources.  Microscopy  of isolate revealed that strain KM221 is gram-positive, catalase-positive,  rod, spore-forming bacterium, motile,  opaque, hair-like outgrowth and unpigmented colonies. The bacterium could not be identified on the basis of its carbon-source-utilization pattern, but a partial sequencing of the 16S rDNA analysis suggest that this strain is closely related to Bacillus iodinum.The ability to degrade MCPA herbicide was examined qualitatively in EMBA indicator medium.  This bacterium grew exponentially with MCPA as  the sole source of energy and carbon. The maximum growth rate (m max)  and the  saturated concentration on MCPA (Cs) were determined to be  0.8024 h-1 and  5.10 mg/l MCPA, respectively.

Copyrights © 2003






Journal Info

Abbrev

coastdev

Publisher

Subject

Education

Description

The Journal of Coastal Development (ISSN 1410-5217) is dedicated to all aspects of the increasingly important fields of coastal and marine development, including but not limited to biological, chemical, cultural, economic, social, medical, and physical development. The journal is jointly published ...